The Effect And Its Mechanism Of Clearing And Purging Heat And Turbid Toxins On Hyperuricemia And Acute Gouty Arthritis

Objective To explore the effect and its mechanism of clearing and purging heat and turbid toxins on hyperuricemia and acute gouty arthritis.Methods 1. Hyperuricemia model was established by intraperitoneal injection with oteracil potassium suspension.2. The effect of clearing and purging heat and turbid toxins on blood uric acid and liver xanthinoxidase of model rice was observed.3. The effect of clearing and purging heat and turbid toxins on urine volume, concentration and amount of urinary uric acid of model rats.4. The effect of clearing and purging heat and turbid toxins on gait, weight, feet swelling degree and mediators of inflammation of model rats were examined.Results 1. Blood uric acid level of model mice increased markedly after intraperitoneal injection with oteracil potassium suspension, while decreased significantly after administration of hypouricemic drug.2. The method of clearing and purging heat and turbid toxins could decrease the concentration of blood uric acid and activity of xanthinoxidase of model mice(P<0.01).3. The urine volume and amount of urinary uric acid of the decoction of Glabrous Greenbrier Rhizome and prepared rhubarb group were the most and that of the decoction of clearing and purging heat and turbid toxins fomula group was the second. Concentration of urinary uric acid of the decoction of Glabrous Greenbrier Rhizome and prepared rhubarb group and benzbromarone group were increased markedly.4. Model rats of acute gouty arthritis limped severely and the ankle circumference of model group increase markedly.72 hours after modeling, the ankle circumference of model group and the decoction of clearing and purging heat and turbid toxins fomula group decreased slghtly, while that of indometacin group increased slightly which was significantly different from that before modeling(P<0.01). The weight of indometacin group were reduced. And there was no significant difference between the weight gaining of the blank group and the decoction of clearing and purging heat and turbid toxins fomula group(P>0.05). Both clearing and purging heat and turbid toxins fomula and indometacin could reduce the elevated level of TNF-?and IL-1?in joint tissue of model rats. While the effect of the two drugs on MMP-3 was not obvious. And the two drugs could both relieve the inflammatory cells infiltration in joint tissue of model rats.Conclusions 1. Animal model of hyperuricemia could established by intraperitoneal injection with oteracil potassium suspension and thus helps to select hypouricemic drug.2. Blood uric acid level of model rice of hyperuricemia could be decreased by method of clearing and purging heat and turbid toxins. Its mechanism may be related to reducing the level of liver xanthinoxidase. The method of clearing and purging heat and turbid toxins could increase the level of urine volume, concentration and amount of urinary uric acid of model rats of hyperuricemia and Glabrous Greenbrier Rhizome and prepared rhubarb may play a main role.4. Decoction of clearing and purging heat and turbid toxins fomula could improve anckle joint function of model rats of Acute gouty arthritis. And it could relieve the inflammatory cells infiltration and reduce the elevated level of TNF-?and IL-1?in joint tissue of model rats. While it has no effect on digestive function of model rats and its effect on MMP-3 was not obvious.