Genome-wide Identification And Functional Analysis Of Genes Related To Cotton Oil

Cotton is an important textile crop and also serves as an important oil crop in China.Cottonseed is rich in fatty acids and protein,which was widely used in processing of edible oil,industrial materials and biodiesel.In this study,BCCP,GPAT and CIPK were taken as research objects to comprehensively analyze their evolution and functions in cotton.The main research results were as follows:1.GbBCCP5 and GbBCCP7 were located on chloroplast,overexpression of GbBCCP5 or GbBCCP7 in yeast and Arabidopsis could increase the oil content of yeast and Arabidopsis seeds,silencing GbBCCP5 or GbBCCP7 genes in H7124 could reduce cottonseed oil content,and overexpression of GbBCCP5 in cotton could increase the cottonseed oil content.2.Genome-wide identified and comparative analysis of GPAT genes in cotton was performed based on the genome data of G.raimondii,G.arboreum,G.hirsutum and G.barbadense.At the same time,the members of GhGPAT gene were co-located with the QTLs related to oil content or protein,and the correlation analysis between the non-synonymous mutational SNPs of GhGPAT gene and the oil content of the land-sea population was analyzed.The results showed that two SNPs of the GhGPAT16 and GhGPA T26 genes were significantly correlated with cotton oil content in one environment of G.hirsutum × G.barbadense,and the sequence variations in most GhGPAT genes were not associated with natural variations in cotton oil content.Overexpression of five GhGPATs could change the oil content and fatty acid content of Arabidopsis seeds.Our results provide a solid foundation for further understanding cotton GPAT genes in the natural variation of cottonseed oil content,and also could provide the candidate genes for function studies.3.A comprehensive analysis of the evolution of CIPK in the Gossypium hirsutum and G.barbadense,and their roles in cottonseed oil.The results showed that fourteen GhCIPK genes were co-localized with oil or protein content quantitative trait loci(QTLs),Five SNPs from four GhCIPKs were found to be significantly associated with oil content in one of G.hirsutum × G.barbadense.Although most GhCIPK genes were not associated with natural variations in cotton oil content,overexpression of the GhCIPK6 gene reduced the oil content and increased C18:1 and C18:1+C18:1d6 in transgenic cotton.In addition,we predicted the potential molecular regulatory mechanisms of the GhCIPK genes.Our results provide the foundation for studying of the molecular mechanism of cotton CIPK gene in regulation of cottonseed synthesis.