| The binding of bis(1,10-phenanthroline)Cu(I);Binding specificity was primarily at the level of triplets and quartets. The trimer TAT was consistently the most preferred sequence with strong cleavage occurring at the central adenosine. The trimer, TGT, and the tetramer sequences, TAGT, CAGT, and TAAT, were moderately to strongly preferred. Preferential cleavage was also observed for the pentamer CAAGC.;An oligonucleotide duplex containing TAT was strongly cleaved at the central adenosine. Duplexes containing C(:G)AT, TGT, TAC(:G), and TAC(:I) all showed significantly reduced cleavage. Duplexes containing C(:I)AT and TIT were cleaved at level nearly equivalent to that for the TAT duplex. These results support a favored intercalation site at TA steps as the primary determinant of Phen;The mono-complex, PhenCu;An induced, conservative circular dichroism spectrum and increases in the viscosity of DNA solutions upon addition of Phen... |
