| Available from UMI in association with The British Library.; The primary aim of this investigation was to evaluate the use of supercritical fluid chromatography and allied techniques for the analysis of veterinary drugs.; Chapter 1 provides an overview of supercritical fluid chromatography/mass spectrometry. The history of SFC, comparison with other chromatographic techniques, the relative merits of capillary and packed column SFC and the use of different stationary and mobile phases are discussed.; Chapter 2 provides a discussion of the use of a novel stationary phase in supercritical fluid chromatography. The use of graphite for chromatography, the reasons why porous graphitic carbon was developed in this area and its use in liquid chromatography are detailed.; Chapter 3 concerns the use of supercritical fluid chromatography/mass spectrometry for the analysis of sulphonamides. Their separation on several silica based stationary phases is discussed and it was found that an aminobonded silica provided the best results.; Chapter 4 is essentially a continuation of Chapter 3 where similar analytical methods were applied to a variety of unrelated veterinary drugs and manipulation of different methodologies overcame problems associated with off-line SFC.; Chapter 5 describes the preliminary evaluation of an analytical method for synthetic oestrogens and trimethoprim with minimum sample preparation. The history and development of supercritical fluid extraction and tandem mass spectrometry are described. An on-line analytical method involving SFE/SFC/MS/MS was used to screen spiked porcine kidney samples. Use of multiple reaction monitoring in the mass spectrometric step meant that 1ppm of trimethoprim, diethylstilbestrol and dienestrol could be readily seem. Application of the method to screen lasalocid in chicken liver revealed some of the limitations of the technique. (Abstract shortened by UMI.)... |
