| We hypothesized that the contribution of amino acids to hepatic gluconeogenesis would increase when glucose demand was increased in ruminants. Our first approach was to establish a sheep model for increased glucose demand. Four wethers injected with excipient or.5, 1.0, or 2.0 g/d of phlorizin excreted an average of 0,72.7, 97.9, and 98.5 g/d of glucose into the urine. Wethers injected with 1.0 g/d of phlorizin excreted approximately 50% of their probable daily glucose production into their urine and adapted by 24 h after the first injection. Phlorizin-induced changes in plasma variables suggested increased gluconeogenesis from amino acids. In experiment 2, isolated hepatocytes from 10 Dorset wethers injected with excipient or 1.0 g/d of phlorizin for 72 h were used to determine the effects of increased glucose demand on conversion of (1-... |
