Biochemical and biophysical studies of wild-type and active-site mutants of human adenosine deaminase

Posted on:1997-02-06

Degree:Ph.D

Type:Dissertation

University:Louisiana State University and Agricultural & Mechanical College

Candidate:Zeng, Qing

Full Text:PDF

GTID:1464390014484541

Subject:Molecular biology

Abstract/Summary:

he focus of this work is structural and functional studies of wild-type and active-site mutants of human adenosine deaminase. Two active-site amino acids, Glu217 and His238, were previously substituted with alanine by site-directed mutagenesis. The Glu217Ala and His238Ala mutants showed little or no changes in Zn content and...

Keywords/Search Tags:

Mutants, Active-site

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