NMR studies of the paramagnetic active complexes of bleomycin

Bleomycin is an antitumor agent used to treat human cancer. It is comprised of metal- and DNA-binding domains linked by a peptide and a disaccharide unit. Fe(II)-bleomycin reacts with O;In the last few years it has become possible to determine the three-dimensional structure of biological macromolecules in solution by NMR methods. This is mainly due to advances in NMR technology such as the development of high-field spectrometers, new two- and three-dimensional NMR techniques, and the application of the NMR techniques to paramagnetic complexes. One and two-dimensional NMR experiments were used to study the metal coordination environment of the Fe(II) and Co(II) bleomycin derivatives. The ligands to both paramagnetic metal centers were identified. NMR techniques were also applied to investigate the binding of Fe(II)BLM to the oligonucleotide d(CCAGGCCTGG)...