Part ?Up-regulation of E-cadherin by saRNA and the activating mechanism Purpose:To detect the expression of E-cadherin gene in renal cell carcinoma(RCC)and investigate the correlation between expression level of E-cadherin and the occurrence of RCC.To identify that saRNA could stimulate E-cadherin expression in ACHN and 786-O cells.Materials and methods: 15 pairs of RCC tissues and adjacent non-tumor kidney tissues were collected and then the E-cadherin expression level of these tissues were detected by immunohistochemical technique and real-time quantitative PCR(RT qPCR).After dsControl,dsEad-215 and siEcad+dsEcad-215 were used to transfect into RCC cell lines ACHN and 786-O respectively,the expression level of E-cadherin mRNA and protein were investigated by RT qPCR and Western blot.Results: Immunohistochemistry and RT qPCR showed that expression level of E-cadherin was down-regulated in RCC tissues compared to adjacent non-tumor tissues(P<0.05).After dsEcad-215 transfected into RCC cell lines ACHN and 786-O,E-cadherin mRNA and protein expression were stimulated by dsEcad-215 via RNAa.Conclusions: E-cadherin was low expressed in human RCC tissues and RCC cells.SaRNAs could activate E-cadherin expression in ACHN and 786-O cell lines by targeting promoter.Part ? sa RNA inhibit renal cell carcinoma cells by activating E-cadherin expressionPurpose:To investigate whether saRNA could up-regulate E-cadherin in RCC cell lines and then inhibit cells invasion and migration.Materials and methods: After ds Control,ds Ead-215 and si Ecad+ds Ecad-215 were used to transfect into RCC cell lines ACHN and 786-O respectively,cell migration and invasion were evaluated by using transwell assay and wound healing assay.Results: Compared with mock and ds Control,ds Ecad-215 made a potent inhibiting effect on migration and invasion capability of the both ACHN and 786-O cells using transwell assay(P<0.05).The similar results were also obtained with wound healing assay(P<0.05).Conclusions: Ds Ecad-215 inhibited RCC cells(ACHN and 786-O)migration and invasion depended on up-regulation of E-cadherin expression. |