The Effects Of Extracorporeal Shock Waves On The Treatment Of Postoperative Infections In Fractures

Objective: To study the effects of extracorporeal shock waves on the treatment of postoperative infections in fractures in vitro and in vivo and evaluate its clinical value.Methods: We chose SD rats as our subjects and use the most common pathogen staphylococcus aureus as the infection source.Through experiments in vitro and in vivo to verify the effects of extracorporeal shock waves on the treatment of postoperative infections in fractures and evaluate its clinical value.After the formation of SD rat's osteomyelitis models and confirmed by high-resolution lateral radiographs.The objects were divided into four groups: Blank(without ESW or Vancomycin),ESW(0.96 m J/mm2 with 4000 impulses),Vancomycin(0.5%,128 ug/ml),ESW combine with Vancomycin.In vitro,we evaluated the results by CLSM and SEM;in vivo,we used the spread plate method and analyzed the CFU/tibia to estimate the anti-infective effects.Results: In vivo,osteomyelitis models of SD rats were successfully built and confirmed by high-resolution lateral radiographs.In vitro,we can see Staphylococcus aureus clearly developed on the surface of self-made stainless steel by CLSM and SEM.After different treatments of each group and analyzed the results in vitro and in vivo,we found that the anti-infective effect ESW combine with Vancomycin > Vancomycin > ESW >Blank.(p < 0.01)Conclusion: The application of ESW combined with vancomycin has obviously anti-infective effect in vitro and in vivo.ESW has synergetic effect with vancomycin against postoperative infections in fractures caused by staphylococcus aureus.