The Integration Chip Of Cancer Detection Based On Microfluidic

In the past decades years,microfluidic technology has developed into a widely interdisciplinary technology.Especially,cancer detection is one of important application.This paper carried out an integrated detection techniques based on a series of key microfluidic technology integrating the detection of cancer markers in the blood.The integration testing requirements for cancer markers designed a variety of microfluidic modules.Each of modules separately can be integrated requirements of background,including ease of integration of efficient micromixer,a plasma separation chip and cancer markers detection chip.Finally three different functions microfluidic chip were integrated by using stacking process,the successful implementation of multi-index integration detection of cancer markers.The micromixers were designed which based on enhancing secondary flow of curve effect.The new micro-mixer comprised of several enhanced curve micro-channel.Secondary flows in curve micro-channels are induced that augment diffusive transport and finally promote enhanced mixing efficiency in considerably shorter downstream distances as comparing with conventional structure.We discussed the mixing efficiency of s serise of micromixer.We compared mixability of optimized enhanced curve type micromixer with additional four micromixers including straight,serpentine,spiral and multi-spiral.We also evaluated mixing efficiency of serpentine micromixers with different geometrical parameters including radius of curvature,curve length and curvature frequency.Discussed the plasma separation chip robust,clogging-free advantage of high aspect ratio bar-shaped structure.The successful use of SU-8 negative plastic processing technology,a high 600 ?m,width of 200 ?m,3000 ?m long dam has a high aspect ratio structure over the shape;double package made of PDMS with plasma separation chip has a strong technology compatibility and robustness.By extracting blood plasma of different size,speed and blood dilution ratio study injections,and injection method to give a series of experimental parameters,set the foundation for later integration into the sample detection parameters.Design,processing,independent verification of the measuring system,an array of three-electrode electrochemical immunoassay chip for six gastric cancer associated marker concentration.In order to reduce the difficulty of processing and electrochemical immunosensor integration,we have designed a set of six independent three-electrode array sensing electrodes.The use of gold as a pseudo-reference electrode can greatly reduce the immune sensor processing difficulty,Cyclic voltammetry showed that such a three-electrode system can be applied to detect cancer markers.Six of gastric cancer related markers were tested linear range,the linear range of the six markers meet the design requirements.We developed a disposable easy-to-use electrochemical microfluidic chip combined with multiple antibodies against six kinds of biomarkers(carcinoembryonic antigen(CEA),carbohydrate antigen 19-9(CA19-9),Helicobacter pylori CagA protein(H.P.),P53oncoprotein(P53),pepsinogen I(PG I)and PG-II).The six kinds of biomarkers related to gastric cancer can be detected sensitively and synchronously in a short time.The specially designed three electrodes system enables cross-contamination to be avoided effectively.In conclusion,the electrochemical microfluidic chip for detecting multiple biomarkers has great potential in applications such as early screening of gastric cancer patients,and therapeutic evaluation,and real time dynamic monitoring the progress of gastric cancer in near future.Finally,the stacking of microfluidic chip was successfully fabricated with versatile modular features.This chip integrated samples mixing,the plasma filtration and cancer biomarkers detection function.Each of modules was fully able to meet the testing requirements.Experimental results showed that the biomarkers in the mice sample in the detection chamber can be captured on the electrode surface by using immunoreactions,and the blank control group can conclude that the sample contains biomarkers.Because of the diversity and complexity of the real component of the blood sample,quantitative detection of biomarker concentrations have some difficulties.In summary,modular design and stackable integrated,microfluidics-based cancer detection chip integration can be successfully detected in blood markers,which for a variety of cancer markers on the integration microfluidic chip lay solid foundation conducive to integrated microfluidic chip are widely used in cancer detection.