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Correlation between the prognostic factors of breast cancer including ER,PR,HER-2 and Ki-67 and intravoxel incoherent motion MR imagingObjective:To explore the correlation between the prognostic factors of breast cancer including estrogen receptor(ER),progesterone receptor(PR),human epidermal growth factor receptor 2(HER-2)and cell proliferation antigen marker Ki-67 and intravoxel incoherent motion diffusion-weighted MR imaging(IVIM).Materials and methods:152 patients with suspicious breast cancer who were treated in our hospital from August 2015 to August 2018 were selected,all the patients received T2W1 fat suppression sequence,IVIM scan and dynamic enhancement(DCE-MRI)scan,the expression of ER,PR,HER-2 and Ki-67 in tumor tissues of all patients were detected by calculation of apparent diffusion coefficient(ADC),D value,D*value,F value and other parameters.Results:According to the exclusion and enrollment criteria of the study,90 cases of breast cancer were finally enrolled,among the 90 cases,73 cases were positive for ER patients,accounting for 81.92%,17 cases were negative for ER patients,accounting for 18.89%.70 cases were positive for PR patients,accounting for 77.78%,20 cases were negative for PR patients,accounting for 22.22%.33 cases were positive for HER-2 patients,accounting for 36.67%,57 cases were negative for HER-2 patients,accounting for 63.33%.59 cases were positive for Ki-67 patients,accounting for 65.56%,31 cases were negative for HER-2 patients,accounting for 34.44%.There were no statistical differences between the positive and negative expression of ER and PR for ADC value,D value,D*value and f value(P>0.05),there was no statistical difference between the positive and negative expression of HER-2 for ADC value and f value(P>0.05),the D value and D*value of the positive expression of HER-2 were higher than the D value and D*value of the negative expression of HER-2(P<0.05).There was no statistical difference between the positive and negative expression of Ki-67 for D*value and f value(P>0.05),the ADC and D value of the positive expression of Ki-67 was lower than the ADC and D value of the negative expression of HER-2(P<0.05).Spearman correlation analysis results:ADC value and ER factors were negatively correlated,D value and HER-2 factor were positively correlated,negatively correlated with Ki-67,D*value and HER-2 factor were highly positively correlated(P<0.05).Conclusion:According to the inter-group differences in the expression of breast cancer immune factors ER,her-2 and ki-67,and the correlation between ADC value and ER,D value and her-2,ki-67,D*value and her-2,the expression of some breast cancer immune factors can be predicted and the prognosis of patients can be analyzed.Part ? Evaluation of The Ability of Diffusion Kurtosis MR Imaging in Breast Cancer Classification and its Correlation with Prognostic Factors Purpose:To assess the ability of diffusion kurtosis imaging(DKI)in invasive breast cancer grading and to evaluate the potential association between DKI-derived parameters:MK/MD and breast cancer clinical-pathologic factors:Estrogen receptor(ER),progesterone receptor(PR),human epidermal growth factor receptor 2(HER-2),antigen identified by monoclonal antibody Ki-67(Ki-67).Materials and methods:168 patients with suspicious breast cancer who were treated in our hospital from August 2015 to August 2018 were selected,DKI(with b values of 0,1000,2000 sec/mm2)data were acquired.Breast lesions were histologically characterized and DKI related parameters-mean diffusivity(MD)and mean kurtosis(MK)—were measured.The MD and MK in grade ? and grade ? were compared by One-way analysis of variance(ANOVA).Kurtosis and diffusion coefficients from DKI were measured by two radiologists.Results:According to the exclusion and enrollment criteria of the study,108 cases of breast cancer were finally enrolled,MK were significantly higher in grade ? breast cancer than in grade II breast cancer(0.99 ± 0.31 vs 0.85±0.23,respectively;P<0.05).MD were significantly higher in grade II breast cancer than in grade ? breast cancer(1.17 ± 0.24 vs 0.83 ± 0.21 P<0.05).MD values were significantly different in Ki-67 expression(1.01 ±0.24 vs 1.10± 0.16),ER expression(1.05± 0.14vs 1.22 ± 0.32),PR expression(0.78 ±0.29 vs 1.15 ± 0.26).But MK values only showed significant difference in Ki-67 expression(0.90 ± 0.15 vs 1.09 ± 0.24,P<0.05).Conclusion:DKI quantitative parameters have important application value in the evaluation of breast cancer classification,MD value and MK value are different between partial immune factor groups,and there is a certain correlation with the expression of ki-67.DKI can provide valuable information in evaluating the proliferation activity of breast cancer tumor cells.MD value and MK value can be used as the indicator of prognosis factors of breast cancer,and indirectly predict the prognosis and treatment effect of breast cancer.Part ? RNA Interference of Ki-67 and HER-2 on the metastasis and invasiveness of breast cancer cellsObjective:To study the interference effect and long-term effect of siRNA on breast cancer cells by in vitro experiments,and provide experimental basis support data for siRNA technology for breast cancer treatment.Materials and methods:The diagnosis of breast cancer patients was performed by DKI and IVMI techniques,and samples of patients with high expression of ki-67 and HER-2 were selected.The primary tumor cells of breast cancer were prepared by tissue block culture method,and purified by trypsin digestion method to establish a tumor cell bank.Based on the coding sequences of human HER-2 and Ki-67 genes,specific HER-2 siRNA,Ki-67 siRNA plasmid and GFP-independent sequence plasmid were constructed using the pslience vector plasmid.The plasmid was transfected into the primary tumor cells by the kit,and the primary tumor cells were used as the negative control.The GFP-containing plasmid was used as the positive control,and the HER-2 siRNA sequence was the HER-2 interference group and the Ki-67 siRNA was included.The sequence is the Ki-67 interference group.The changes of ki-67 and HER-2 mRNA expression levels,cell cycle changes,tumor cell apoptosis and invasive ability were observed before and after siRNA interference.Results:After transfection with siRNA,the transfection efficiency in the Ki-67 and HER-2 interference groups increased with time,showing an increasing trend,reaching a maximum at 96 h and then easing.No fluorescence appeared in the primary cell group,and the positive control group showed significant fluorescence.Using the highest transfection rate(96h)as a sample,RT-PCR was used to detect the inhibitory effect of siRNA on the expression of ki-67 and HER-2 mRNA in breast cancer cell lines of 28 successful patients.Primary cells There was almost no difference between the group and the positive control group.The expression of mRNA in Ki-67 group was significantly inhibited by interference of Ki-67 siRNA.The expression of mRNA in HER-2 group was significantly inhibited by interference of HER-2 siRNA.Compared with the negative control group,the number of G0/G1 cells in the siRNA interference group was significantly increased,while the number of cells in the S phase was significantly decreased.There was no significant difference between the G2/M phase cells and the negative control group.There was no significant difference between the positive control group and the negative control group.The number of cells in the siRNA-interfering group through the chamber artificial basement membrane(Matrigel)was significantly reduced compared with the negative control group and the positive control group,respectively,and was statistically significant.The cell scratch test results showed that the migration rate of the primary cell group and the positive control group at 96 h was significantly higher than that of the Ki-67 interference group and the HER-2 interference group.It indicates that the migration ability of cells is significantly affected after SiRNA interferes with tumor cells.Conclusions:siRNA technology can effectively reduce the growth and proliferation of tumor cells,promote tumor cell apoptosis and reduce the invasiveness of tumor cells. |
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