Whole Transcriptome Analysis Of Mechanical Tension On Human Skin RNA Expression Profile

Objective:A new generation of high-throughput sequencing technique was used to analyze the transcript group sequencing of skin tissue cells regenerated under mechanical tension and normal skin tissue cells,and to explore the effect of mechanical tension on the regeneration of skin tissue cells and normal skin tissue cells by means of a new generation of high-throughput sequencing technique.The differential gene expression between expanded skin tissue cells and normal skin tissue cells during regeneration,in order to reveal the regulation mechanism of genes in the transcription group during the division and proliferation of expanded skin cells.To further understand the gene expression regulation of skin biological growth at a new level.Method:Four cases of Chinese nationality aged 18～25 years were collected,all of which were patients with frontal skin expansion and scar repair.After 3 and 6 months of water injection expansion,the expansion multiple is between 2 and 3 times.A full thickness skin of about 0.5cm × 0.5cm was taken from the experimental group and the control group.Intraoperative sampling was placed in a cryopreservation tube and liquid nitrogen was immediately used for quick freezing.Sample RNA was extracted.In this study,the expression profiles of mRNA,lncRNA,circRNA and microRNA in skin epidermal cells after accelerated differentiation and proliferation under mechanical tension were compared with those of normal epidermal cells by transcriptome sequencing,and the correlation between the full transcriptome and the normal epidermal cells was analyzed.Results:1.Through the comparative analysis of mRNA expression profile under mechanical tension and mRNA expression profile of normal epidermal cells,a total of 18912 mRNA were identified.There were 1470 mRNA differentially expressed between the experimental group and the control group.Compared with the control group,the expression of lncRNA was up-regulated and the expression of lncRNA was down-regulated in the experimental group.2.Through the analysis of the sequencing data of the transcription groups of the experimental group and the control group,a total of 6568 lncRNA,were identified,most of which were intronic-lncRNAs（6.5%）.There were 53 lncRNA differentially expressed between the experimental group and the control group.compared with the control group,the expression of lncRNA in the experimental group was up-regulated in 22 cases,and the expression of lncRNA was down-regulated in 31 cases.3.Based on the sequencing data of the experimental group and the control group,a total of 1039 circRNAs,were identified,of which 126 were newly discovered circRNAs.1032 cirRNAs were the same in the experimental group and control group,4 cirRNA were only expressed in the experimental group,only 3 cirRNAs were expressed in the control group,and 48 circRNAs were differentially expressed between the experimental group and the control group.Compared with the control group,the expression of circRNAs was up-regulated in 26 cases and down-regulated in 22 cases in the experimental group.4.Through the data analysis of transcription group sequencing in the experimental group and the control group,a total of 1403 known miRNA mature bodies and 1194 precursors were identified.the novel miRNA in the samples was predicted,and a total of 67 mature bodies and 70 precursors were found.There were 40 miRNA differentially expressed between the experimental group and the control group.compared with the control group,the expression of circRNA in the experimental group was up-regulated and the expression of circRNA was down-regulated in 12 cases.5.The expression profiles of IncRNA,circRNA and microRNA obtained in this study were analyzed jointly:a total of 184 lncRNA-miRNA pairs were obtained,of which 24 pairs were down-regulated by miRNA lncRNA and 74 pairs were down-regulated by miRNA up-regulated lncRNA.There were 207 pairs of circRNA-miRNA relationships,of which the most miRNA were associated with hsa_circ₀000230.A total of 466 miRNA-mRNA pairs were obtained,of which 180 pairs were down-regulated by miRNA,62 pairs were up-regulated by miRNA,and 98 pairs were related to hsa-miR-671-5p and mRNA.Conclusion:Expanded skin tissue and normal skin tissue have their own characteristic gene expression profiles,and the possible genes that regulate the biological growth of skin under mechanical tension have been screened out.The expression profiles of mRNA,lncRNA,circRNA and microRNA differential genes were established,which provided a theoretical basis for subsequent genetic,proteological and molecular biology research.