Analysis Of FSN Treatment Of Rheumatoid Arthritis Based On Transcriptomics Technique

Objective: In this paper,we used i TRAQ proteomics and transcriptomics to explore the mechanism of rheumatoid arthritis(RA)in the treatment of rheumatoid arthritis(RA)from rheumatoid and differential gene,in order to find the model of cold and wet,CIA and CIA +(P <0.05),in order to explore the difference between cold and dampness model group and the effect of FSN capsules on the CIA model and the potential role of FSN capsules in the treatment of RA,to study the mechanism of FSN in the treatment of RA.Methods: ? A composite model combining cold and wet model,CIA model and composite model was established.The bioinformatics technique was combined with i TRAQ/TMT(isotope labeling relative and absolute quantification)technique combined with liquid phase tandem mass spectrometry to identify dampness,CIA,composite three model The difference protein of the mouse serum,the gene protein Ontology,the Kyoto gene and genome encyclopedia(Kyoto Encyclopedia Genes and Genomes)function of the annotation and enrichment analysis,the use of STRING protein interaction database to obtain significant difference protein network Mutual relationship,screening to find the cold syndrome and CIA model related to the differential protein.? SPF grade SD rats were randomly divided into normal group,CIA group,compound model group,FSN low dose group,FSN middle dose group,FSN high dose group,Tofacitinib group,Normal group and model group were given the same amount of saline,the FSN of each dose group 1 times,Tofacitinib,Ceron rheumatoid1 day,were 28 consecutive days.Twenty-eight days after modeling,the rats in each group were observed.The arthritis of rats was observed by HE staining.The levels of RF,CRP and IL-1? were detected by enzyme-linked immunosorbent assay(ELISA),IL-6,TNF-? and other related inflammatory cytokines were observed.The therapeutic effect of FSN capsules on different model rats was observed.? The rats in the normal group,the CIA model group,the composite model group,the FSN-CIA group and the FSN-compound group were analyzed by immunohistochemistry.The differentially expressed genes were analyzed by means of the Illumina Hi Seq 4000 platform.GO analysis and pathway pathway analysis were performed to screen the key differential expression genes of FSN.Results: ? The differentially expressed protein profiles were identified by i TRAQ mass spectrometry combined with bioinformatics techniques by comparative analysis.For the comparison of the two samples,the experiment was up-regulated or down-regulated by 1.3 times,ie,the difference between the groups was> 1.3(up-regulated)or <0.77(down-regulated),and P <0.05 was the significant difference standard.The A total of 435933 mass spectrometry,2230 peptide fragments and 540 protein spots were identified by the combination of four groups of serum(normal control group,CIA model group,dampness model group and compound model group),and 518 proteins There is a quantitative value.There were 125 proteins in the cold and dampness model group and 35 patients in the cold and wet group.The difference was significant in the CIA model group and the control group.The difference was 27 in the CIA group and 66 in the CIA group.There were 190 differentially expressed proteins in the group and 152 in the compound group,and 38 were down-regulated in the composite group and 217 in the model group and the model group.The difference was significant in the combined group and the CIA group,Up 208,down 37.Differentially expressed proteins in the bioinformatics,and RA related to the occurrence of inflammation,immune response,cell structure,protein,signal transduction and other aspects of the function.The pathways involved are related to RA,which are mainly complement systems,and the activation of the coagulation system.The effects of dampness on the serum protein of CIA model are HSP90AA1,ALDOA,PKM,RAC1,VWF,C2,HSP90B1,HSPD1,PSMA3,PSMA6,PSMA4,PSMA2 and so on.In the interconnection of protein network and related pathways The key role is the key node of RA.? From the overall condition of the rats,the CIA model group and the compound model group showed swelling,water and food intake,lethargy,apathetic,dull and dull,slow and loose stools.Compared with the normal group,synovial hyperplasia,the formation of pannus,cartilage and bone tissue by infiltration also associated with newborn;joint swelling,swelling significantly increased;rheumatoid factor,C-reactive protein levels and inflammation associated with inflammatory cytokines The level was significantly higher.Compared with the CIA model group,the synovial hyperplasia and joint swelling and swelling were significantly increased in the model group.The level of inflammatory cytokines was significantly increased.The FSN capsule treatment group had better mental status compared with the model group,,The improvement of the driving force,the improvement of the stool of the stool,the reduction of the joint swelling and the decrease of the joint swelling in the FSN capsule group.After 28 days,the mental state of the rats in the administration group had different degrees of improvement,the drinking water and the food intake increased,FSN drug treatment group can reduce the proliferation of synovial cells,inflammatory cell infiltration can be seen to varying degrees of reduction,joint pathological features improved,the treatment group of peripheral blood in the RF group,the frequency of synovial cell proliferation in the model group,slow action and other symptoms significantly improved;,CRP and inflammatory cytokines IL-1?,IL-6,TNF-? levels were also significantly reduced.? In this study,5 samples of CIA and compound synovial tissue of FSN capsules were sequenced by Illumina Hi Seq 4000,and 4.48 Gb data were obtained per sample.Finally,9070 new transcripts were detected,Of which 4084 belonged to the new variable splice subtype of the known protein coding gene,2600 transcripts belonging to the new protein coding gene,and the remaining 2386 belonged to long chain non-coding RNA.CIA model group and compound model group,normal group and CIA model group,normal group and compound model group,compound model group and compound model group,CIA model group and compound model group,normal group and CIA model group,normal group There were 278,118,84,65,56,37,4 and3 differentially expressed genes in the CIA model group,the normal group and the composite model group,the CIA group and the CIA model group.The expression of differentially expressed genes in the CIA model group and the combined model group and the CIA group and the CIA model group were up-regulated by 54,1,65 and 30,2and 0 respectively One.GO analysis and pathway analysis of differentially expressed genes showed that most of the differentially expressed genes were enriched in cell processes,development,metabolism,immunization,biological process regulation,stimulatory response,cell signaling,cell components,cell Outer region,catalytic activity,binding activity,and the like.Pathway pathway analysis showed that most of the differentially expressed genes were enriched in cell communities,transport and catabolism,protein folding and degrading lipid metabolism,immune diseases and so on.FSN treatment of RA-related genes are: FDFT1,SQLE,AKAP13,KCNMB1,UBB,RPS27 A and so on.Conclusion:? In this study,the differential expression proteins of three model rats were identified by i TRAQ / TMT labeled with liquid phase tandem mass spectrometry for the first time.The differentially expressed proteins could be used in the influence of cold and dampness factors on CIA model an important role.? FSN capsules can significantly improve the physiological parameters of IL-1?,IL-6,TNF-?,RF and CRP in rats with rheumatoid arthritis.? In this study,the differentially expressed genes of synovial tissue of rats before and after FSN capsules were identified by transcriptome sequencing.The differentially expressed genes may be the key targets for FSN capsules.