| Objective:(1)To study the differences and distribution of fat content of pancreatic head,body part and tail in patients with firstly-diagnosed type 2 diabetes.(2)To evaluate the feasibility of the IDEAL-IQ for quantification fat content of pancreas in patients with type 2 diabetes.Materials and Methods: There were 91 cases of patients with type 2 diabetes(37 females and 54 males),aged 24-69 years,with an average age of 46 years.The range of BMI was 22.22-26.74 kg/m~2,with an average of 23.48 kg/m~2.The fasting blood glucose was 7.23 mmol/L-24.40 mmol/L,with an average of 8.32 mmol/L.91 patients with firstly-diagnosed type 2 diabetes were recruited in this study.Pancreas was scanned with iterative decomposition of water and fat with echo asymmetry and least squares estimation quantification sequence(IDEAL-IQ)and proton magnetic resonance spectroscopy(~1H-MRS),respectively.Fat content in the head,body,tail of the pancreas was measured with the related software technique in post-processing workstation,and to compare the differences of fat content of pancreatic head,body part and tail in analysis of variance.Pancreas fat fraction was measured with the IDEAL-IQ and ~1H-MRS by linear regression analysis,and Pearson correlation coefficients were calculated.The Bland-Altman 95% consistency limit were calculated both the IDEAL-IQ and ~1H-MRS.When P<0.05,the difference was statistically significant.Results :The fat fraction in the head?body and tail of the pancreas was(8.49±2.20)%,(8.81±2.29)% and(8.42±2.04)%,respectively,by IDEAL-IQ scanning and there was no statistical difference among them(F=2.734,p=0.067).The fat fraction in the head?body and tail of the pancreas was(10.94±4.97)%,(10.48±4.15)% and(10.92±5.84)%,respectively,by ~1H-MRS scanning and there was no statistical difference among them(F=2.982,p=0.058).The mean value of fat fraction in the pancreas was(8.57±2.12)% by IDEAL-IQ measuring and the fat fraction of the pancreas was(10.78±4.87)%,respectively.The IDEAL-IQ was positively correlated with ~1H-MRS in the measurement of pancreatic fat content(r=0.729;P=0.001).Base with bland-altman analysis,the two were also highly consistent,with 95.17% of the points within the 95% consistency limit.Conclusions: Fat content in the head?body and tail of the pancreas was basically the same,and there were no differences in the distribution of fat content of pancreatic head,body part and tail in patients with firstly-diagnosed type 2 diabetes.The IDEAL-IQ and ~1H-MRS have a good correlation and high consistency in the fat ration of pancreas in patients with firstly-diagnosed type 2 diabetes.Objective: To study the differences of fat content of pancreas and liver in the patients with firstly-diagnosed type 2 diabetes,the patients whose the glucose tolerance reduced and the population of normal blood sugar.and to discuss the differences and correlation of fat content of pancreas and liver in three groups.To analysis the relevance between the fat content of pancreas and liver in the patients with firstly-diagnosed type 2 diabetes,the patients whose the glucose tolerance reduced and the healthy volunteers of normal blood sugar,and body mass index(BMI),triglyceride(TG),fasting plasma glucose(FPG),fasting insulin(FINS),glycosylated hemoglobin(Hb A1c),total cholesterol(CHOL),low density lipoprotein(LDL),alanine aminotransferase(ALT),aspertate aminotransferase(AST),steady state model of insulin secretion index(HOMA-?),insulin resistance index(HOMA-IR).Method: 91 patients with firstly-diagnosed type 2 diabetes and 31 patients whose the glucose tolerance reduced,37 healthy volunteers of normal blood sugar,whose fat contents in the pancreas and liver were checked with IDEAL-IQ.The interest area was placed in the fat score map of the post-treatment station,the fat fraction of the pancreas,body,tail and liver were measured,and the average fat fraction of pancreas and liver were calculated.To compare the fat score difference on average of three groups of samples of liver and pancreas,to analyze the correlation between the fat contents in the pancreas and liver and insulin resistance,islet beta cell function,and laboratory indexes of correlation,and between the fat contents of the pancreas and liver.Results:(1)The fat fraction of the pancreas and liver was(8.57±2.12)% and(14.08±2.37)% in patients with firstly-diagnosed type 2 diabetes,respectively.The fat fraction of the pancreas and liver was(6.59±1.29)% and(9.48±1.65)% in patients whose the glucose tolerance reduced,respectively.The fat fraction of the pancreas and liver was(4.37±1.53)% and(5.38±1.32)% in healthy volunteers of normal blood sugar,respectively.The fat contents of the liver and pancreas in patients with firstly-diagnosed type 2 diabetes were higher than that in patients whose the glucose tolerance reduced and healthy volunteers of normal blood sugar,and the difference was statistically significant.The fat contents of the pancreas in patients whose the glucose tolerance reduced were higher than that healthy volunteers of normal blood sugar,and the difference was statistically significant.The fat contents of the liver in patients whose the glucose tolerance reduced were higher than that healthy volunteers of normal blood sugar,and the difference was no statistically significant.(2)TG(P<0.001),CHOL(P<0.001),LDL(P=0.003),FBG(P<0.001),Hb A1c(P=0.002),FINS(P<0.001),and HOMA-IR(P<0.001)of type 2 diabetes group were higher than glucose tolerance group and normal blood glucose control group.HOMA-?(P<0.001)was lower than the glucose tolerance group and the normal blood glucose control group.The TG(P=0.002),CHOL(P<0.001),LDL(P<0.003),FBG(P<0.001),Hb A1c(P<0.001),FINS(P=0.004),and HOMA-IR(P<0.001)of glucose tolerance reduction group were higher than the normal control group,and Homa-beta(P<0.001)was lower than normal blood glucose ontrol group.There was no statistically significant difference between Age,BMI,ALT and AST(P> 0.05).(3)The pancreatic fat content in patients of type 2 diabetes group and glucose tolerance reduction group were positively correlated with FBG(r=0.541,P=0.003;r =0.502,P=0.001),CHOL(r=0.484,P=0.001;r =0.345,P=0.004),TG(r=0.335,P=0.013;r=0.306,P=0.021),FINS(r=0.516,P=0.001;r=0.402,P=0.001)and LDL(r=0.426,P=0.002;r=0.304,P=0.023),Hb A1c(r=0.409,P=0.002;r =0.326,P=0.004),not related to ALT and AST(r=0.216,P=0.351;r =0.123,P=0.236);Pancreatic fat content of normal blood glucose group were positively correlated with TG(r = 0.385,P = 0.007),CHOL(r = 0.547,P = 0.011),LDL(r = 0.359,P = 0.359),not related to FBG(r = 0.128,P = 0.128),FINS(r = 0.124,P = 0.553),Hb A1c(r = 0.294,P = 0.154),ALT(r = 0.302,P = 0.302),AST(r = 0.219,P = 0.219).The fat content of the pancreas in the three groups was not correlated with Age and BMI(r=0.038,r=0.042,r=0.216,P >0.05).(4)The liver fat content in patients of type 2 diabetes group and glucose tolerance reduction group were positively correlated with FBG(r=0.663,P=0.003;r =0.526,P=0.001),CHOL(r=0.512,P=0.001;r =0.480,P=0.002),TG(r=0.496,P=0.003;r =0.385,P=0.001),FINS(r=0.628,P=0.000;R =0.415,P=0.001)and LDL(r=0.614,P=0.003;r =0.523,P=0.001),Hb A1c(r=0.518,P=0.001;r =0.512,P=0.001),ALT(r=0.643,P=0.003;r =0.513,P=0.003),AST(r=0.612,P=0.002;r=0.528,P=0.001).The liver fat content of normal blood glucose group were positively correlated with ALT(r = 0.433,P = 0.001),AST(r = 0.418,P = 0.418),not related to FBG(r = 0.228,P = 0.458),Hb A1c(r = 0.294,P = 0.273),FINS(r = 0.224,P = 0.356),TG(r = 0.403,P = 0.257),CHOL(r = 0.384,P = 0.185),LDL(r = 0.295,P = 0.295).The liver fat content of three groups of subjects were not associated with Age,BMI(P > 0.05).(5)The pancreatic fat content in patients of type 2 diabetes group and glucose tolerance reduction group were positively correlated with HOMA-IR(r=0.418,P=0.010;r =0.396,P=0.005),and were negatively correlated with HOMA-?(r=-0.525,P=0.001;r=-0.598,P=0.002).The liver fat content in patients of type 2 diabetes group and glucose tolerance reduction group were positively correlated with HOMA-IR(r=0.516,P=0.002;r=0.601,P=0.001),but were not related to homa-?(r=0.298,P=0.068;r =0.317,P=0.072).Conclusion: the fat contents of the pancreas and liver in firstly-diagnosed patients with type 2 diabetes and patients whose the glucose tolerance reduced were higher than that of healthy volunteers of normal blood sugar.The fat contents of the pancreas and liver were not correlated with each other in the firstly-diagnosed patients with type 2 diabetes,patients whose the glucose tolerance reduced,and healthy volunteers of normal blood sugar.The relationship between the fat content Liver and pancreas,type 2 diabetes mellitus and glucose tolerance is close.The increased fat content liver and pancreas may be the risk factors for type 2 diabetes mellitus and glucose tolerance to reduce.Objective: To study the relationship between mir-19a-3p and pancreatic beta cells,and to investigate the role of mir-19a-3p in diabetes treatment.Materials and methods: collected blood samples from patients with type 2 diabetes(n=45)and normal subjects(n=20),determined glucose levels in blood samples,and analyzed plasma SOCS3 levels by ELISA.Cells were cultured in islet cell lines,ins-1 and MIN6,cells transfected,and untransfected cells were used as controls.Total RNA was extracted using Trizol.Use mir Vana ? real-time rt-pcr mirna detection kit mi R-19a-3p relative to express a.Using standard double chain chimeric fluorescence staining quantitative PCR testing SOCS3 m RNA relative expression,use 2-? ? Ct quantitative relative expression method.The relative expression quantity of the protein was analyzed with the Image J software,and the results were compared with that of GAPDH.The insulin level was measured by enzyme-linked immunoassay,and the insulin secretion was evaluated after glucose stimulation.Then the cell proliferation assay and apoptosis detection were performed,and the bioinformatics analysis was carried out with Target Scan 4.2 online software to predict the assumed target sequence of mir-19a-3p.The luciferase activity was determined by a biluciferase report system,and the activity of luciferase and firefly luciferase was determined by the biluciferase reporter system.Statistical analysis: the data of at least 3 independent experiments were expressed in the form of mean or minus standard deviation.Statistical analysis was performed using Graph Pad Prism 5 software.T test or single factor variance analysis was used to compare the differences.Pearson correlation coefficient was used to analyze the relationship between mir-19a-3p level and blood glucose or SOCS3 level.P value <0.05 was considered statistically significant.Results the expression of mir-19a-3p in diabetic patients was significantly down-regulated.Mirna-19a-3p promotes the proliferation and insulin secretion of pancreatic beta cells and inhibits apoptosis.Islet cell SOCS3 is the target gene of mir-19a-3p.SOCS3 was involved in the regulation of the proliferation and apoptotic insulin secretion of mir-19a-3p.The expression level of SOCS3 in diabetic patients increased,and its plasma level was inversely proportional to mir-19a-3p.Conclusion: mir-19a-3p plays an important role in islet cell function,and mir-19a-3p /SOCS3 axis may be a potential therapeutic target for diabetes. |
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