The Study Of The Roles Of IGF-1 And IGFBP-3 Polymorphisms In NONFH And Chinese Constitution

Part IThe Study of the roles of Insulin-Like growth factor-I(IGF-1)and Insulin-like growth factor-binding protein-3(IGFBP-3)polymorphisms in non-traumatic osteonecrosis of the femoral head(NONFH).Objective:To investigate the roles of Insulin-Like growth factor-I(IGF-1)and Insulin-like growth factor-binding protein-3(IGFBP-3)polymorphisms in non-traumatic osteonecrosis of the femoral head(NONFH).Methods:Case group included 130 NONFH patients who admitted to First School of Clinical Medicine,Guangzhou University of Chinese Medicine from October 2014 to December 2016,and control group enrolled 111 healthy people who had physical examinations during the same period in First School of Clinical Medicine,Guangzhou University of Chinese Medicine and Linyi People’s Hospital.Polymerase Chain Reaction –Restriction Fragment Length Polymorphism(PCR-RFLP)was used to detect the polymorphisms of IGF-1rs2946834,IGF-1 rs2195239,IGFBP-3 rs924140,and IGFBP-3 rs2453839.Results:The TT genotype and T allele of IGF-1 rs2946834 was lower in the case group as compared to the control group(both P < 0.05).Meanwhile,the GG,AG+GG and G allele in IGFBP-3 rs924140 were significantly lower in the case group than those in the control group(all P < 0.05).Haplotype CCAT,CGAC,and CGAT were proved to be risk factors for NONFH.IGF-1 rs2946834 and IGFBP-3 rs924140 polymorphisms were related to the disease course,clinical staging,and extentof disease of NONFH(all P < 0.05).Conclusions:Polymorphisms of IGF-1 rs2946834 and IGFBP-3 rs924140 may relate to the risk of NONFH.Part IIThe Study of the roles of Insulin-Like growth factor-I(IGF-1)and Insulin-like growth factor-binding protein-3(IGFBP-3)polymorphisms in Hemostasis type of Chinese Medicine of non-traumatic osteonecrosis of the femoral head(NONFH).Objective:To investigate the roles of Insulin-Like growth factor-I(IGF-1)and Insulin-like growth factor-binding protein-3(IGFBP-3)polymorphisms in Hemostasis type of Chinese Medicine of non-traumatic osteonecrosis of the femoral head(NONFH).Methods:Case group included 130 NONFH patients who admitted to First School of Clinical Medicine,Guangzhou University of Chinese Medicine from October 2012 to December 2013,and control group enrolled 111 healthy people who had physical examinations during the same period in First School of Clinical Medicine,Guangzhou University of Chinese Medicine.Case group is divided into2 groups accourding to the Chinese medicine body type: Hemostasis group(80patients)and non-Hemostasis group(50 patients).Results:The compare was conducted on the genetic site of IGF-1 rs2946834 between hemostatis group and non-hemostatis group.Significant differences in the distribution of genetic type and alle genes were found between hemostatis group and control group(P<0.05).There are no significant differences between non-hemostatis group and control group(P>0.05)。Comparing the hemostatis group and non-hemostatis group no significant difference were detected in the distribution of genetic type and alle gene.The compare was conducted on the genetic site of IGF-1 rs2195239 between hemostatis group and non-hemostatis group.Significant differences in the distribution of genetic type and alle genes were found between hemostatis group and control group(P<0.05).There are no significant differences betweennon-hemostatis group and control group(P>0.05).Comparing the hemostatis group and non-hemostatis group no significant difference were detected in the distribution of genetic type and alle gene.The TT genotype and T allele of IGF-1 rs2946834 was lower in the case group as compared to the control group(both P <0.05).Meanwhile,the GG,AG+GG and G allele in IGFBP-3 rs924140 were significantly lower in the case group than those in the control group(all P < 0.05).Haplotype CCAT,CGAC,and CGAT were proved to be risk factors for NONFH.IGF-1 rs2946834 and IGFBP-3 rs924140 polymorphisms were related to the disease course,clinical staging,and extent of disease of NONFH(all P < 0.05).Conclusions:Polymorphisms of IGF-1 rs2946834 and IGFBP-3 rs924140 may relate to hemostatis type of NONFH in chinese medicine.