Research On Antiaging Activity And Mechanism Of Blueberry Polyphenols

Aging is one of the fatal factors that may cause diseases including: cardiovascular and neurodegenerative diseases.Ample researches showed that dietary fruit could delay the aging related diseases.Blueberries,one of the Ericaceous Vaccinium plants,are rich source of dietary bioactivities compounds including flavonoid,phenolic.Considering that aging may induce by irradiation,oxidative stress and inflammation,the activities of blueberry phytochemicals were detected in vitro and in Caenorhabditis elegans.In the present study,the phenolic compound profile,antioxidant,anti-inflammation,anti-irradiation,anti-oxidative stress and anti-aging of blueberry varieties were also investigated.We compared the phytochemical profiles in 14 varieties of blueberry using HPLC.According to the experimental results,12 compounds were analyzed.Antioxidant activity of the blueberry extracts(BE)were determined by oxygen radical absorbance capacity(ORAC),peroxyl radical scavenging capacity(PSC)assay and cellular antioxidant activities(CAA).It was confirmed that the BE had high antioxidant activity in different methods,and the antioxidant activity is relatively different among various varieties.Anti-inflammation activities of BE were investigated using lipopolysaccharide(LPS)activated the RAW264.7 cells.BE could significantly inhibit the gene expression of IL-1β,TNF-a,IL-6 and iNOS.The protein expressions of NLRP3 and Caspase-1 in the blueberry treated groups were significantly lower than the LPS treated group.BE had the anti-inflammation activities through NLRP3 inflammasome.Ultraviolet irradiation is a negative factor that induces skin damage,inflammation,and aging.In this study,we also investigate the anti-ultraviolet effect of BE in human skin keratinocyte(HaCaT)and Human foreskin fibroblast(HFF)cells.Ultraviolet irradiation could increase the expression of the inflammatory factors such as TNF-α,IL-1β,IL-6,IL-8,and BE could decrease the trends.In contrast to HFF cells,which secreted MMP-1 in response to UVC irradiation,no accumulation of MMP-1 was observed after UVC irradiation in HaCaT cells.Surprisingly,the expression of the MMP-1 was significant increase in co-culture models than the alone culture models in UVC-irradiation group.BE could decrease the expression of the MMP-1 in HFF cells co-culture models.The same trend applied to expression of mRNA such as of MMP-1,c-fos and c-jnu.Moreover,BE partly recovered the MAPK(mitogen-activated protein kinases)pathways including: MMP-1,c-fos,c-jnu protein.Blueberry is a rich source of bioactive phytochemicals which may enhance the beneficial effects in health.The Caenorhabditis elegans(C.elegans)models were used to detect the anti-aging activities of the BE in this study.The mean lifespan of C.elegans treated with BE(50,100 and 200 mg/m L)was increased significantly by 22.2 %,36.5 % and 44.4 % in a dose-dependent manner.In addition,supplement of the diet with BE increased the healthspan,such as improved motility and decreased the lipofuscin accumulation.C.elegans pretreated with BE were more resistant to stressors(such as heat,UVB and paraquat).Furthermore,antioxidant gene: sod-3,cat-1,mev-1,skn-1,sek-1 and nhr-8 were up-regulated after BE treatment.Our findings revealed that the blueberry extracts played a significant role in prolong lifespan,improved healthspan of the C.elegans.