Association Study Between The Vatiamin D And Xinjiang Uygur Type 2 Diabetes

Objective:(1)To determine the prevalence of hypovitaminosis D and the possible risk factors associated with hypovitaminosis D in patients with type 2 diabetes mellitus(T2DM)in Xinjiang Han and Uygur population.(2)To evaluate the serum vitamin D levels and its association with nutritional status and diabetic complications of DM in Xinjiang Uygur T2 DM patients.(3)To explore the association of genetic polymorphisms in VDR gene and T2 DM susceptibility in Xinjiang Uygur population.Methods:(1)Clinical data of 2551 hospitalized Han and Uygur T2 DM patients without vitamin D supplement from January 1,2013 to December 31,2014 were collected.The correlation between serum 25-(OH)D levels and its risk factors were investigated.(2)According to serum 25-(OH)D level,Uygur T2 DM patients with 25-(OH)D deficiency were divided into two groups: those with severe vitamin D deficiency and those with non-severe vitamin D deficiency.(3)We genotyped VDR polymorphisms(rs2228570,rs1544410,rs7975232,rs731236)using im LDRTM multiple SNPS typing kit in Xinjiang Uygur T2 DM cases and T2DM-negtive controls.Result:(1)Overall,the mean vitamin D concentration was 11.94±7.55ng/mL.The prevalence of vitamin D deficiency was 86.32%,vitamin D insufficiency was 11.09%,and vitamin D sufficiency was 2.59%.Uygur T2 DM patients had significantly lower serum 25(OH)D levels than Han T2 DM patients.After controlling for covariates in multiple logistic regression analysis,younger age,being female,ethnic Uygur,less sunshine hours,higher glucose and TG level were independent predictors of 25-(OH)D deficiency.(2)The prevalence of 25-(OH)D deficiency was higher in Uygur T2 DM patients.25-(OH)D was extremely deficient in 66.9% patients,and only1.7% patients had normal 25-(OH)D level.severe vitamin D deficient group had higher fasting blood glucose,glycosylated hemoglobin,body mass index,and higher prevalence of multiple atherosclerotic plaques,peripheral neuropathy than non-severe vitamin D deficient group.(3)Uygur T2 DM group had significantly lower level of vitamin D than control group(P<0.05).The distribution of allele C of VDR gene rs2228570 was significantly higher in Uygur T2 DM group than that of control group.Multiple Logistic regression analysis demonstrated that Fok I(rs2228570)polymorphism in VDR gene was associated with T2 DM susceptibility.Conclusion:(1)The prevalence of Hypovitaminosis D is higher in type 2 diabetes patients in Xinjiang Han and Uygur population.(2)There are serious vitamin D malnutrition in Xinjiang Uygur T2 DM patients.The nutritional status of vitamin D in Uygur T2 DM patients is associated with higher fasting blood glucose,glycosylated hemoglobin,and higher prevalence of atherosclerosis and neuropathy.(3)VDR gene Fok I(rs2228570)polymorphism was associated with Uygur T2 DM in Xinjiang,genotype C/C may be a susceptibility gene for type 2 diabetes in Uygur nationality.VDR gene polymorphism is associated with central obesity,suggesting a mechanistic link between VDR gene polymorphism and T2 DM susceptibility.