| Background : "Eighteen antagonisms"— the controversial prohibited combinations in traditional Chinese medicine? Some physicians believe that with the use of the anti- drug cause side effects,so Eighteen antagonisms should be banned; while others believe physicians with the use of anti- drug can treat chronic ills. Recent studies showed that the Eighteen antagonisms are conditional prohibited combinations. Theses researches were mostly aimed at single medicinal combinations. However, instead of single combination, it is prescription that was more often used in clinic. While there are some doctors used composite recipe removed one or two anti- drug in clinical, there are a number of physicians used composite recipe, but did not establish anti- drug,so there is no way to research the efficacy of the resulting combination is associated with the anti-drug prescription. So on the one hand to meet the requirements of clinical applications, on the other hand , we need to examine the compound produced the "efficiency" is associated with the anti- drug combination,based on previous studies,we chose a dose close to clinical application,valid and not showed significant toxicity with gansuigancao specific ratio , specific doses and different varieties in gansuibanxia soup to do experimental study.Purpose: we chose a dose close to clinical application , valid and not showed significant toxicity with gansuigancao specific ratio, specific doses and different varieties in gansuibanxia soup to do experimental study. We removed one or two anti- drug of gansuibanxia soup to compare with gansuibanxia soup to observe its effect on ascites in rats. We can further investigate the"effect" of gansuibanxia soup is associated with the anti- drug combination. Whether remove one or two anti-drug of the prescription will produce efficiency, effectiveness or toxicity reduction (by poison ),attenuated role as a guide clinicians safe and reasonable use of licorice anti- drug combination euphorbia provide scientific support, in order to enrich and improve the scientific basis "Eighteen against" incompatibility theory . These studies can provide a scientific basis for clinicians to use the anti- drug combination of gansuigancao and the research scientific can provide theory to support to use "Eighteen antagonisms"Method:? Early researches had studied acute toxicity of Gansui?Gancao?the compatibility of gansui and gancao?Gansuibanxia soup. The results showed that the use of Cugansui ( powder blunt ) and gancao (frying with honey ) compatibility compared with long-term heavy use of single- dose group had a certain smell increased toxicity effects, but the compound with Cugansui ( powder blunt ) and gancao (frying with honey ) had no significant toxic effects. So in order to explain the toxicity of single herbs , anti- drug combinations and the compound,we choose the group of Gansuibanxia soup?Gansuibanxia soup removed Zhigancao?Gansuibanxia soup removed Cugansui, Gansuibanxia soup removed Zhigancao and Cugansui,Zhigancao, Cugansui and the compatibility of Zhigancao and Cugansui to evaluate the toxicity of single herbs, anti-drug combination, and the compound containing anti-drug combination by dismantling the compound containing anti-drug combination of Gansui and Gancao. We can verify toxic or effective response from the anti- drug combination of Gansui and Gancao or from Gansui,Gancao.?We have completed the experimental study of the effect of Gansuibanxia soup with different proportion and doses of Gansui and Gancao on ascites rat model. In previous experiments, seven different proportion and high, medium and low three different doses of Gansui and Gancao in Gansuibanxia soup, the results showed that the group of matched group four and the group of low dose have better efficacy and do not show significant toxicity. Whether the"efficacy" of the group of matched group four and the group of low dose are related to the anti-drug combination of Gansui and Gancao, whether the "efficacy" of Gansuibanxia soup is related to the anti-drug combination of Gansui and Gancao, these questions require us to verify experimentally. The doses of Zhigancao and Cugansui in the group of matched group four are 3.467g and 0.39g, the doses transformed adult dosage are 34.67g and 3.9g. The doses of Zhigancao and Cugansui in the group of low dose of Gansuibanxia soup are 1.1g and 16.7g."People's Republic of China Pharmacopoeia · a " prescribed amount of Gansui is 0.5-1.5g,Zhigancao dosage is 2-10g.The doses of Cugansui and Zhigancao in the group of matched group four and the group of low dose are close to the doses of " People's Republic of China Pharmacopoeia · a ".This topic is based on the clinical application of conditions to investigate the application conditions of anti- drug combinations containing the Zhigancao and CUgansui, so we chosed the group of matched group four and the group of low dose to do experiments.? Early results of our topics showed that Gansuibanxia soup containing anti- drug combination of Zhigancao and Sshenggansui has the better diuresis , while other aspects of heart and kidney showed no significant toxicity, but Gansuibanxia soup containing anti- drug combination of Zhigancao and Cugansui? Shenggancao and Shenggansui?Shenggancao and Cugansui also have the better diuresis as mean while showed toxicity on the aspects of heart and kidney. So We need to remove one or two drug of anti- drug combination to investigate the"effect"and "toxicity" of gansuibanxia soup is associated with the anti- drug combination.Results:1. Experiment of Acute toxicity or the LD50(n)There is no LD50 of every group which was treated by different durg. The mice were fed with gansuibanxia soup removed zhigancao, gansuibanxia soup removed cugansui, gansuibanxia soup removed zhigancao and cugansui and cugansui soup for 28 days, however, it could not to be up to the LD50, so the maximum dose was definited the maximum tolerated dose which were 60.87?78.8?80g?10g/Kg respectively. The LD50(n) of the groups which was treated by gansuibanxia soup, zhigancao and zhigancao added cugansui respectively were 51.01?48.26?44.21g/Kg respectively. The relevant indicators included ALT?AST?ALP?BUN?CRE of liver and kidney of undied mice were detected by taking eyeball blood after the test of LD50(n). The results showed the application of single cugansui, single zhigancao and the compatibility of cugansui and zhigancao compatibility effects on liver and kidney function mainly in the compound for a certain degree of liver damage. The application of gansui gancao anti-drug combination in compound had less damage to liver than the application of single gansui gancao.However, the test of LD50(n) showed the application of gansui gancao anti-drug combination in compound had higher LD50(n) than the application of single gansui gancao. The other drugs of compound may had the effect of increaseing the LD50(n) on the application of single gansui gancao. It was said The other drugs of compound decreased the toxicity or damage on liver of the application of single gansui gancao.2. The biological effect and mechanism of preferred gansuibanxia soup plus or minus gansuigancao anti-drug combination on rats with ascites(1) Toxicity CharacterizationCardiac function: The cardiac index of the proportion of the whole party group was below the model group and the proportion of the whole party removed zhigancao group and the difference is significant in statistics (p<0.05), however, each administration group had no significant effect on the CK and DH of serum. The cardiac pathological changes of the whole party group was lighter compared with the untreated group, the pathological damage of the proportion of the whole party group had a little decrease than the the proportion of the whole party removed zhigancao group, the proportion of the whole party removed cugansui group and the proportion of the whole party removed caosui group.Liver function:The rat liver index of the proportion of the whole party group was lower than the model group, the whole party removed cugansui group and the proportion of the whole party removed zhigancao group and the difference is significant in statistics (p<0.05 or p<0.01). The content and A/G ratio of serum ALB of the proportion of the whole party group higher than the model group and the whole party removed cugansui group, the difference is significant in statistics(p<0.05), so was the whole party removed zhigancao group but had no significant difference. The content of rat plasma treated by the whole party group and the whole party group removed cugansui group were more than the model group and the difference is significant in statistics(p<0.05 or p<0.01). The rat plasma treated by the whole party group had more SOD content than the whole party group removed zhigancao group(p<0.05). The content of rat plasma treated by the whole party group had the trend of increase compared to the whole party removed cugansui group and the proportion of the whole party removed caosui group, but had no significant difference.The fatty degeneration of the liver tissue and Inflammatory cell infiltration of the proportion of the proportion of the whole party had a decrease compared to the model group,the proportion of the whole party removed zhigancao group, the proportion of the whole party removed cugansui group and the proportion of the whole party removed caosui group.Renal function: The kidney index of the proportion of the whole party was decreased than the model group and the difference is significant in statistics (p<0.05). The other administration group had a decrease compared to the model group but had no significant difference. The content of serum BUN of the proportion of the whole party group had a decrease compared to the model group, the proportion of the whole party group removed zhigancao group, the proportion of the whole party group removed cugansui group and the proportion of the whole party group removed caosui group, the difference is significant in statistics (p<0.05). The content of serum CRE of the proportion of the whole party group, the proportion of the whole party group removed zhigancao group, the proportion of the whole party group removed cugansui group and the proportion of thewhole party group removed caosui group had a increase compared to the model group and the difference is significant in statistics (p<0.01), the content of serum CRE of the proportion of the whole party group had a increase trend compared to the proportion of the whole party group removed cugansui group and the difference is significant in statistics (p<0.01), the pathological changes such as kidney tissue tube and fibrosis of the proportion of the whole party group had a decrease compared to the model group, the proportion of the whole party group removed one ortwo anti-drug.(2) Efficacy evaluationDiuresis effects: The proportion of the whole party group and the proportion of the whole party group removed one or two anti-drug had the effect of increase in body weight of rats,decrease abdominal circumference, increase the amount of urine and reduce the amount of ascites.The proportion of the whole party group had an advantage on the effect of increase in body weight of rats, decrease abdominal circumference, increase the amount of urine and reduce the amount of ascites, but had no significant difference. On the effect of reduce plasma aldosterone and antidiuretic hormone, the proportion of the whole party group had an advantage but had no significant difference.Immunomodulatory: The proportion of the whole party group had more IgA than the proportion of the whole party removed caosui group in rat ascites and had the significant difference(p<0.05), The proportion of the whole party group and the proportion of the whole party removed caosui group had less IgA than the model group in rat ascites and had significant difference, The proportion of the whole party removed zhigancao group and the proportion of the whole party removed cugansui group had a trend of decrease of IgA compared to the model group in rat ascites but had no significant difference, The proportion of the whole party group had less IgG than the model group in plasma and had the significant difference(p<0.05) and the proportion of the whole party removed caosui group in plasma and had the significant difference(p<0.01).The proportion of the whole party removed zhigancao group and the proportion of the whole party removed cugansui group had a trend of decrease of IgG in plasma but had no significant difference.The proportion of the whole party group had less IL-6 than the proportion of the whole party removed cugansui group and the proportion of the whole party removed caosui group in rat ascites and had the significant difference(p<0.05 or p<0.01).(3) MechanismLiver: The expression of rat liver CYP2E1 mRNA of the proportion of the whole party group were less than the model group and had a significant difference(p<0.05 or p<0.01) and the expression of rat liver CYP2E1 mRNA of the proportion of the whole party group were less than the proportion of the whole party removed cugansui group and the proportion of the whole party removed caosui group(p<0.01). However, the expression of CYP2E1 and CYP2E1 mRNA were inconsistent; The proportion of the whole party group, the proportion of the whole party removed zhigancao group, the proportion of the whole party removed cugansui group and the proportion of the whole party removed caosui group had an trend of increase of the expression of rat liver CYP3A1 mRNA and had a significant difference(p<0.05 or p<0.01); The proportion of the whole party group had an trend of increase of the expression of rat liver CYP3A1 mRNA compared to the proportion of the whole party removed cugansui group and the proportion of the whole party removed caosui group and had a significant difference(p<0.05 or p<0.01).Kidney: The expression of rat kidney CYP2E1 mRNA of the proportion of the whole party group were less than the model group and the proportion of the whole party removed zhigancao group and had a significant difference(p<0.05 or p<0.01). The expression of rat kidney CYP2E1 mRNA had no significant difference among the proportion of the whole party, the proportion of the whole party removed cugansui group and the proportion of the whole party removed caosui group. The apoptosis of the proportion of the whole party, the proportion of the whole party removed zhigancao group and positive drug were easier than the model group(p<0.01). The proportion of the whole party had a trend of decrease compared to the proportion of the whole party removed zhigancao group , the proportion of the whole party removed cugansui group and the proportion of the whole party removed caosui group and there is a significant difference between the proportion of the whole party and the proportion of the whole party removed caosui group (p<0.05 or p<0.01).3. Gansui banxia soup subtracted gansui gancao against drug combination close to clinical doses was selected to investigate the biological effect and mechanism of ascites rats(1) Toxicity characterizationCardiac function: function: Low doses gansuibanxia soup group and the whole party subtracted gancao and gansui group in rats cardiac index was lower than the model group and was statistically difference (p<0.01). Low doses of gansui banxia soup respectively subtracting zhigancao and cugansui in rat cardiac index compaed with the model group was not statistically difference (p > 0.05). At the same time, low doses of gansui banxia soup group in rats cardiac index comparede with low dose of gansui banxia soup respectively subtracted zhigancao,cugansui and gancao gansui groups has a lower trend and was lower than that of gansui banxia soup subtracted zhigancao group and was Statistically significant (p < 0.05). But rats serum CK and LDH content of low dose all party group has not obvious effect compared with low dose gansui banxia soup subtracting one or two herbal medicine groups.Liver function: low doses of gansui banxia soup group in the rat liver index was lower than the model group and was statistically difference (p < 0.05). And low dose of the whole party group subtracted zhigancao, cugansui and gancaogansui separately in rat liver index compared with model groups was not statistically difference (p > 0.05). At the same time, low doses of gansui banxia soup group in rats liver index comparede with low dose of gansui banxia soup group subtracted zhigancao ,cugansui and gancao gansui respectively has a lower trend and was lower than gansui banxia soup subtracted gancao gansui group and was Statistically significant (p< 0.05). the effect of low doses of gansui banxia soup group in reducing the rat serum AST/ALT ratio, increasing the content of erum ALP,ALB, TP and the content of plasma SOD and GSH on the whole is better than that of low doses of gansuibanxia soup group subtracted zhigancao,cugansui and gancao gansui separatelyRenal function: low doses of gansui banxia soup group and gansui banxia soup subtraced cugansui and gancao gansui separately in the rat renal index was lower than the model group and was statistically difference (p < 0.01). low doses of gansui banxia soup group in the rat renal index was lower than gansui banxia soup subtracted zhigancao group and was statistically difference (p< 0.05). The content rats serum BUN of low doses gansuibanxiasoup group is lower than the model group and was statistically difference (p < 0.05), but the model group in the content of rats serum BUN compared with low doses gansuibanxia soup subtracted zhigancao, cugansui and gancao gansui separately was not statistically difference (p < 0.05),the content of the rats serum BUN of low doses of gansui banxia soup groupt was lower than that of gansui banxia soup subtracted gancao gansui and was statistically difference (p < 0.05).(2) Efficacy evaluationDiuresis effect: On the fourth day after Drug delivery, compared with model group ,the rats weight of positive medicine group, low dose group and low dose subtracted blindly or two medicine was higher and was statistically difference (p < 0.01, or p < 0.05), and the rats weight of low doses group was higher than that of low dose subtracted zhigancao and cugansui separatly and was statistically significant (p < 0.05). On the eighth day after Drug delivery,the rats weight of low doses group was higher than that of low dose subtracted zhigancao and cugansui separatly and was statistically significant (p < 0.01, or p < 0.05). On the fourth day after Drug delivery, the ratio of Abdominal circumference/ weight of gansui banxia soup group was lower than of model group, and also lower than that of low dose group subtracted zhigancao and cugansui separately and was statistically significant (p < 0.01, or p< 0.05). The effect of increasing the urine of rats, decreasing in abdomen water, increasing the net weight, reducing plasma renin, angiotensin and aldosterone, antidiuretic hormone levels of low dose group on the whole was better than that of low doses of gansui banxia soup group subtracted Blindly or two drugs separatelyImmunomodulatory: Compared with low doses of gansui banxia soup group subtracted Blindly or two drugs separately, the effect in lowing the content of ALB, IL-6,TNF-a and VEGF in ascites and the content of IgA,IgG,IgM in plasma,increasing the content of IL-2 and rising the whole blood CD3+ counting of low dose group has a certain advantage.(3)MechanismLiver: The Expression of rat liver CYP2E1 mRNA of low dose group was lower than that of the model group and was significant difference (P < 0.05),but gansui banxia soup group separately subtracted zhigancao and cugansui was higher than the Model group and gansui banxia soup group and was significant difference (P < 0.01). The Expression of CYP2E1 protein and CYP2E1 mRNA is consistent. The expression of CYP3A1 mRNA of the low doses group and low dose group removed the blindly against drug or two was higher than that of the model group and was significant difference, and that of the low doses group was higher than the low dose group removed cugansui and gancao gansui separately and was significant difference (p < 0.01 or p<0.05).Kidney: The Expression of rat Kidney AQP-2 mRNA and AQP - 2 protein of low dose group was lower than that of the model group and slso lower than that of low dose group removed zhigancao and was significant difference (P<0.05). The content of LDH, ?2-MG and EGF in rats urinary of low dose group was lower than that of the model group and was statistically difference(p < 0.01, or p < 0.05), and the content of LDH, ?2-MG of low dose group was was lower than that of low dose group removed zhigancao and was significant difference (P<0.05), but the content of EGF in rats urinary of low dose group was lower than that of low dose group removed cugansui and was significant difference (P<0.01). compared with the model group and low dose group removed cugansui and gancao gansui, the tissue apoptosis of rat kidney of low dose group was reduced and was significant difference (p < 0.01 or P<0.05).4. Effect of Gansuibanxia decoction with different varieties of Gansui and Gancao plus-minus Gansuigancao anti- drug combination in malignant ascites rats(1) Toxicity CharacterizationCardiac function: The groups of Gansuibanxia decoction with different varieties of Gansui and Gancao and the groups of Gansuibanxia decoction removed one or two drug of anti- drug combination did not show significant toxicity and efficacy in aspect of cardiac index. In lowering serum CK levels, the whole side group (the compatibility of Zhigancao and Cuansui) was the best,the LDH level of the whole side group (the compatibility of Zhigancao and Shengansui) was lower than the model group and statistically significant (p <0.05 ). The levels of serum CK and LDH of containing other Gansui Banxia soup with varieties of Gansui and Gancao compared with model group does not have a statistically significant difference (p> 0.05). The serum LDH level of the groups of Gansuibanxia soup (the compatibility of Zhigancao and Shengansui) removed one or two drug of anti- drug combination have tendency to increase compared with Gansuibanxia soup(the compatibility of Zhigancao and Shengansui),the serum LDH level of the groups of Gansuibanxia soup (the compatibility of Zhigancao and Shengansui) removed Zhigancao has increased statistically significant difference (p <0.05);Liver function: In improving liver function, the whole side group (the compatibility of Zhigancao and Shengansui) has the better effects on decreasing serum AST , ALT, ALP, AST /ALT, GLB, elevating TP, ALB than the whole side group(the compatibility of Zhigancao and Cuansui?the compatibility of Shenggancao and shenggansui?the compatibility of Shenggancao and Cugansui ),but having no significant difference between them. The whole side group (the compatibility of Zhigancao and Shengansui) has the better effects on the above related indicators than the whole side group (the compatibility of Zhigancao and Shengansui) removed one or two drug of anti- drug combination. The whole side groups(the compatibility of Zhigancao and Cuansui?the compatibility of Shenggancao and shenggansui?the compatibility of Shenggancao and Cugansui) have the worse effects than the whole side groups removed one or two drug of anti- drug combination. In improving liver antioxidant indicators, such as SOD, MDA, GSH aspects, the whole side group ((the compatibility of Zhigancao and Shengansui ) has better effects than the whole side group(the compatibility of Zhigancao and Cuansui?the compatibility .of Shenggancao and shenggansui?the compatibility of Shenggancao and Cugansui). The whole side group (the compatibility of Zhigancao and Shengansui) has the better effects on the above related indicators than the whole side group (the compatibility of Zhigancao and Shengansui) removed one or two drug of anti- drug combination. The whole side groups(the compatibility of Zhigancao and Cuansui?the compatibility of Shenggancao and shenggansui?the compatibility of Shenggancao and Cugansui ) have the worse effects than the whole side groups removed one or two drug of anti- drug combination.Renal function: In the aspect of reduced kidney index, the whole side group (the compatibility of Zhigancao and Shenggansui) was the best, kidney index of the whole side group(the compatibility of Zhigancao and Cugansui) and the whole side group (the compatibility of Shenggancao and Cugansui) were higher than the whole side group (the compatibility of Zhigancao and Shenggansui) and statistically significant (p <0.01 or p <0.05). Kidney index of the whole side group (the compatibility of Zhigancao and Shenggansui) and the whole side group (the compatibility of Shenggancao and Shenggansui) were lower than the whole side group removed one or two drug of anti- drug combination. The serum BUN and CRE levels of the whole side group (the compatibility of Shenggancao and Cugansui) were higher than the whole side group(the compatibility of Zhigancao and Shenggansui); the serum BUN levels of the whole side group(the compatibility of Zhigancao and Shenggansui) removed one or two drug of anti- drug combination was higher than the whole side group, the serum BUN and CRE levels of the whole side group (the compatibility of Shenggancao and Cugansui) removed one or two drug of anti-drug combination were lower than the whole side group (the compatibility of Shenggancao and Cugansui).(2) Efficacy evaluationDiuresis effects:Gansuibanxia soup with different varieties of Gansui and Gancao had a better effect on increasing body weight of rats, the amount of urine, serum sodium, reduce of rat abdominal circumference, the amount of ascites, serum potassium, the content of antidiuretic hormone than Gansuibanxia decoction included shenggancao and zhigansui anti-drug group. The group of Gansuibanxia decoction included shenggancao and zhigansui anti-drug had a worse effect mentioned last sentence and Showed significant toxicity. Gansuibanxia decoction included zhigancao and shenggansui removed one or two anti-drug had a worse effect on the index of diuresis than Gansuibanxia decoction. However, when remove one or two anti-drug,Gansuibanxia decoction included zhigancao and cugansui and Gansuibanxia decoction included shenggancao and cugansui had much better effect on the index of diuresis.Immunomodulatory: Among the gansuibanxia decoction with different varieties of gansui and gancao, gansuibanxia included zhigancao and shenggansui anti-drug had a decrease of the index of spleen compared to the model group, gansuibanxia included shenggancao and shenggansui and gansuibanxia included shenggancao and cugansui and it had a significant difference(p<0.01). Gansuibanxia included zhigancao and shenggansui removed one or two anti-drug had a trend of decrease compared gansuibanxia included zhigancao and shenggansui, but had no significant difference. Gansuibanxia decoction included shenggancao and shenggansui removed shenggancao had a trend of decrease compared to Gansuibanxia included shenggancao and shenggansui and had a significant difference(p<0.05). Gansuibanxia decoction with different varieties of gansui and gancao which remove one or two anti-drug or not had no influence on IL-6,IL-2, TNF-?, IFN-?, VEGF in ascites and CD3+, CD4+, CD8+ in blood.(3) MechanismLiver: All of the gansuibanxia decoction with different varieties of gansui and gancao had the effect on inhibiting the expression of liver CYP2E1 mRNA. Meanwhile, they showed better effect on inhibiting the expression of liver CYP2E1 mRNA than themselves which remove one or two anti-drug. When remove one or two anti-drug, they presented different expression of liver CYP2E1. Gansuibanxia decoction included zhiggancao and shenggansui,gansuibanxia decoction included zhiggancao and cugansui and gansuibanxia decoction included shengggancao and shenggansui could improve the expression of liver CYP3A1 mRNA. However, gansuibanxia decoction removed caosui exhibited the trend of increase compared to the blank group,the model group and gansuibanxia decoction group, they presented different expression of liver CYP3A2 mRNA.Kidney: All of the gansuibanxia decoction included zhiggancao and shenggansui,Gansuibanxia decoction included zhiggancao and cugansui and gansuibanxia decoction included shenggancao and cugansui exhibited the effect on inhibiting the expression of AQP-2 mRNA,gansuibanxia decoction included zhiggancao and shenggansui was more effective than gansuibanxia decoction included shenggancao and cugansui. Gansuibanxia decoction included zhiggancao and shenggansui showed more effective in the expression of AQP-2 mRNA than themselves which was removed one or two anti-drug, however,gansuibanxia decoction included zhiggancao and cugansui was just on the contrary. Gansuibanxia decoction included zhiggancao and shenggansui showed the effect of decreaseing the content of epidermal growth factor and?2-microglobulin and is more effective than themselves which was removed one or two anti-drug.Gansuibanxia decoction included zhiggancao and cugansui and gansuibanxia decoction included shenggancao and cugansui exhibited a trend of increase the content of epidermal growth factor compared to themselves which was removed one or two anti-drug. Gansuibanxia decoction included zhiggancao and cugansui exhibited a trend of decrease the content of ?2-microglobulin compared to themselves which was removed one or two anti-drug. Gansuibanxia decoction included shenggancao and cugansui exhibited a trend of increase the content of ?2-microglobulin compared to themselves which was removed one or two anti-drug.Conclusion:?Effect of Gansuibanxia decoction with different varieties of Gansui and Gancao plus-minus Gansuigancao anti- drug combination in malignant ascites rats. Though the effect of the groups of Gansuibanxia soup with different proportion and doses of Gansui and Gancao and the groups of the whole side group removed one or two drug of anti- drug combination on ascites rat model,the whole side groups have the better effects on Diuresis,immune regulation, improve liver and kidney function , and in terms of regulation of P450 liver enzymes and kidney AQP-2 expression than the whole side group removed one or two drug of anti- drug combination. The whole side group and the whole side group removed one or two drug of anti- drug combination have no obvious toxicity on the related indicators in this experiment. So Gansuibanxia soup removed one or two drug of anti- drug combination played a "less effective"role to the "effect" of Gansuibanxia soup.?The groups of Gansuibanxia decoction with different varieties of Gansui and Gancao have the effect of diuresis and inhibit CYP2E1 mRNA expression, but the whole side group(the compatibility of Zhigancao and Shenggansui) was the best.On the effects of diuresis and inhibit CYP2E1 mRNA expression, the whole side group removed one or two drug of anti- drug combination was worse than the whole side group, that Gansuibanxia soup containing different varieties of Gansui and Gancao removed one or two drug of anti- drug combinationplayed a "less effective " role to the "effect" of Gansuibanxia soup.In improving heart function, liver function, renal function and aspects of renal mechanisms,the whole side group (the compatibility of Zhigancao and Shengansui) has the better effects than the whole side group(the compatibility of Zhigancao and Cuansui?the compatibility of Shenggancao and shenggansui?the compatibility of Shenggancao and Cugansui). The whole side group (the compatibility of Zhigancao and Shengansui) has the better effects on the above aspects than the whole side group removed one or two drug of anti- drug combination,that Gansuibanxia soup(the compatibility of Zhigancao and Shengansui) removed one or two drug of anti- drug combination played a "less effective" role to the "effect" of Gansuibanxia soup. The whole side group( the compatibility of Shenggancao and shenggansui?the compatibility of Shenggancao and Cugansui ) have worse effects on the above aspects than the whole side group removed one or two drug of anti- drug combination, that The whole side group( the compatibility of Shenggancao and shenggansui?the compatibility of Shenggancao and Cugansui) removed one or two drug of anti-drug combination, played a "synergistic" role in improving above functions.The groups of Gansuibanxia decoction with different varieties of Gansui and Gancao have the effect of impro... |
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