Experimental Study Of Behavior Of The Model Of Depression In Rats And Central Neurotransmitters Worry Soup Commonly Used Drug Intervention

Objective:Watch wuyou soup common medicine for:ginseng, angelica, ginseng-zizyphusjujube kernel, the solitary raise and Chronic mild unpredictability stress (Chronicunpredictive mild stress, CUMS) behavioral depression model rats and single amineneurotransmitter in the brain serotonin (5-HT) and norepinephrine (NE), discuss theviewpoints have common medicine for soup, the mechanism of action ofantidepressant effects.Methods:1, model preparation:40male SD clean level only rats, weight180g to220. Withinthe group, according to the weight of rats with picric acid as points after sign,according to the ratio of the total group within a group of simple random sampling isdivided into four groups: normal control group (no treatment), model group, easysoup ginseng, angelica group all ginseng soup zizyphus jujube kernel group,10ineach group. Before the start of building, the rats first adaptive feeding1week. Normalcontrol group5per cage breeding only, not be model preparation. The rest threegroups in isolated rats with chronic mild stress of unpredictability sexual stimulation(CUMS) as a method for depression rat model, concrete behavior, including2hours4degrees swim in ice water for5minutes, banned water24hours,24hours fasting, cliptail upside down for5minutes, light and shade and24hours30volts electric foot fiveseconds, shaking for5minutes,5minutes40degrees high temperature environment,a total of18kinds of stimulus randomly assigned, day1, each stimulus appears2times, the same stimulus can’t continuous appear that animals cannot expectstimulation and stimulation of21consecutive days.2, packet delivery: after the success of the building, all ginseng soup angelica group of2.7g/kg per day, worry-free ginseng soup group of semen ziziphi spinosae (zs)2.2g/kg per day to fill the stomach, the normal control group and model group3ml saline lavage daily, between groups are at8o ’clock in the morning-9, irrigation to1times a day, in the experiment date22consecutive3weeks of administration, once aday.3, index testing: to change the rat body weight, food consumption, consumption ofsugar water and open box experiment as a behavioural indicators, respectively, priorto building model and medication after21days are measured, and in medicine toassess safe soup, ginseng, Chinese angelica, ginseng zizyphus jujube kernel changesin behavior of chronic stress depression model rats. Brain5-HT, the NE contentdetermination in lavage administration after21days, quickly, many rats hadhomogenate after high speed centrifugation, take supernatant fluid, usingenzyme-linked immunoassay (ELISA) for testing, and statistical analysis.Results:1, after21days of lonely after raising and chronic mild stress stimulusunpredictability, compared with normal control group, each module maker behaviorindex of the rats were significantly lower (P <0.05or P <0.01), the difference wasstatistically significant.2, after21days of drug intervention, compared with model group, ginseng, angelicagroup have soup ginseng zizyphus jujube kernel group big mice body weightincreased (P <0.05); Food consumption increased (P <0.05); Activity increased (P <0.05); For partial addicted to sugar water enhanced (P <0.05); Monoamineneurotransmitters in the brain5-HT, NE content increase.Conclusion:1, solitary, combined with chronic mild unpredictable stress stimuli is depressionanimal model is an ideal preparation method. After building model group ratsappeared a lack of interest or pleasure, was characterized with the phenotypes offewer activities, exploring ability and so on in depression behavior, better simulatedthe depression patients in the clinical performance of depressive state. 2, easy soup in medicine for, ginseng, Chinese angelica, ginseng zizyphus jujubekernel, can improve depression in experimental animals behavior, increase singleamine neurotransmitter in the brain5-HT, the content of NE3, easy soup medicine to, in the ginseng, angelica, ginseng has a certain degree ofantidepressant effects of semen ziziphi spinosae (zs), its mechanism of action andpossible increased depression animal model of brain monoamine neurotransmitters5HT, NE in the related content.