| Chapter 1 Epidemiological survey of infants with rotavirus infectionObjective: To support fundamental data of supervision of rotavirus infection, we aim to study the epidemiological characteristics of rotavirus infection in infants in Anhui province.Methods: There were 28578 fecal samples collected for analysis using polymerase chain reaction (PCR). All of these subjects were 0 ?1 year old with diarrhea treated,from January 2014 to December 2010. The positive rate of rotavirus infection was calculated in age, duration and geographic factors. Furthermore, serotype and genotype of rotavirus were analyzed.Results: (1) There is significant difference (X2=206.31 , p< 0.01) between infant aged 0-4 (range: 67.09%?69.76%, mean: 68.57%) months and 4- 12 months (58.08%?69.07%, 59.89%) in positive rate of rotavirus infection. (2) An increasing tendency has been found in rotavirus infection, rising from 58.99% in 2010 to 69.96% in 2014. (3)Although there was no obvious difference in regional distribution, the positive rate in Tongling (71.32%) was the highest, which was dramatically higher than that of Suzhou,Bozhou, and Maanshan (57.51%, 8.43% and 59.68%, respectively). (4) Interestingly,rotavirus infection occurred highly in winter (71.35%, 69.23%- 75.39%) but not in summer (56.98%, 56.14%- 57.66%). (5) G3 (73.78%) was the main serotype in diarrhea infant with rotavirus infection, and the main genotype was P8 (68.92%).Conclusion: PCR is a stable, efficient, sensitive and accurate method, which can be applied to the detection of rotavirus. From 2010 to 2014, the positive rate of infants with rotavirus was 62.82% in Anhui province, and increasing tendency has been found year by year, infection occurred highly in winter and the young infants, rotavirus serotype was mainly G3 (73.78%), and genotype was P[8] (68.92%).Chapter 2 Analysis of prognostic factors of infants with rotavirusObjective: To explore the prognostic factors of infantile rotavirus infection, and provide evidence for the prevention and treatment of infant with rotavirus infection.Methods: 348 acute diarrhea infants with rotavirus infection in our hospital were included in this study, whose prognosis and clinical data, including gender, age, feeding patterns, course of disease , as well as the existence of hepatic, cardiac and respiratory tract infection and CNS damage,were analyzed by univariate analysis and logistic regression.Results: Among 348 cases, 61.49% were cured, 31.32% were improved and the maintenance accounted for 7.18%. Univariate analysis showed that there were significant difference (P< 0.05) in age,course of disease, feeding patterns, liver damage,myocardial damage, respiratory tract infection and CNS damage between the patients with different prognosis. Multivariate Logistic analysis revealed that age, feeding pattern, and CNS damage were associated with prognosis (P< 0.05).Conclusion: Age, feeding patterns, course of disease, liver damage, myocardial damage,respiratory tract infection and central nervous system involvement are the factors which affect the prognosis of infants with rotavirus. Age,feeding pattern and central nervous system involvement may be an independent risk factor for the prognosis of infants with rotavirus, which should be paid enough attention. The prognosis could be improved by strengthen the nursing of young infants, breastfeeding and timely prevention and treatment of central nervous system damage. |
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