| Objective:(1) Analysis of homocysteine(Hcy) and the relationship between traditional risk factors and diet and VTE.(2)To investigate the relationship between MTHFR, MS, MSR gene polymorphism and VTE, and the effect of gene gene and gene environment factors to VTE.(3)From the point of view of inflammation, the relationship between SAA gene and VTE was preliminarily discussed. Methods:(1)A case-control study, Uygur VTE 162 cases?Han VTE 184 cases, healthy sex and age matched Uygur 203 cases?Han 230 cases,clinical data were collected from all participants and food intake, using ELESA method to detect Hcy? folate and vitamin B12 levels.(2)The application of PCR-PFLP technique to detect MTHFR gene C677T? A1298C?MS A2756 G and MSR A66 G polymorphism,analysis,gene polymorphisms and VTE? Hcy;using crossover analysis of gene-gene and gene-environment interaction on VTE.(3)Using ELESA method to detect SAA level,the SAA gene rs12218 and rs4638289 polymorphisms were detected by PCR-PFLP technique. Results:(1)Smoking in Uygur and Han groups in the proportion of patients with VTE were higher, the t test was statistically significant, and by logistic analysis, smoking is an independent risk factor of VTE. Compared with the control groups, smoking makes the risk of VTE in the Uygur population increased by 2.199 times; the risk of VTE in the Han population increased by 1.952 times.(2)In Uygur,the BMI of VTE(27.69±4.07 to 25.77±4.17) kg/m2,waist circumference(85.08±9.60 to 81.54±9.94) cm higher than the control groups, and the difference were statistically significant,P < 0.05;abdominal obesity is an independent risk factor of VTE,OR=4.114, 95%CI:2.242-7.549;In Han,waist circumference(83.31±10.13 to 80.01±10.64) cm higher than the control group, and the difference was statistically significant(P < 0.05), and BMI(24.91±3.63 to 24.45±3.44) kg/m2 were no differences between the two groups, abdominal obesity is also an independent risk factor of Han VTE patients,OR=1.676, 95%CI:1.060-2.650.(3)In the first part, in the Uygur population,the CRP level of VTE population was higher than that in control group(6.44±4.17 vs.4.75±3.54) mg/L, CRP is not an independent risk factor for VTE in Uygur by logistic regression analysis; in the Han population,the CRP level of VTE population was higher than that in control group(6.33±4.34 vs.4.60±3.13)mg/L,CRP is an independent risk factor for VTE in Han by logistic regression analysis(OR=1.174,95%CI:1.100-1.253,P<0.001).(4)Compared with the control group, the levels of Hcy in the VTE group were significantly higher in both the Uygur and the Han people, respectively( 13.36±7.84 vs.11.03±3.92) umol/L and(13.26±4.54 vs.10.56±3.16)umol/L,P<0.05;folic acid levels in all of VTE groups were decreased, respectively( 20.29±8.39 vs.22.57±10.09) nmol/L and( 20.02±7.86 vs.23.31±9.49)nmol/L,P<0.05;The concentrations of vitamin B12 in two groups of Uygur and Han ethnic groups showed no significant differences,P>0.05.(5)Elevated Hcy level is an independent risk factor of Uygur and Han Nationalities.In the Uygur population,high Hcy levels increased the risk of VTE,OR=1.87,95%CI:1.023-3.450;In the Han population,high Hcy levels increased the risk of VTE,OR=2.113,95%CI 1.283-3.480.(6)In Uygur VTE group,daily intake of red and processed meat was higher than the control group(274.79±114.54 vs.203.39±97.709)g/day,by Logistic multivariate analysis,red and processed meat is one of the independent risk factors of Uygur VTE.In Han VTE group,daily intake of red and processed meat was higher than the control group( 201.39±85.66 vs.251.62±10.44) g/day,by Logistic multivariate analysis,red and processed meat is not one of the independent risk factors of Han VTE.(7)In the Han population, daily intake of vegetables in VTE group was less than that in the control group(405.83±101.46 vs.442.89±114.35)g/day,vegetables is a protective factors of VTE, P<0.05.(8) In the Uygur population,the recessive model of C677 T MTHFR was correlated with VTE(OR=2.182,P=0.011),but not with its dominant model(OR=1.359,P=0.406).In the Uygur population,the recessive model of C677 T MTHFR was correlated with VTE( OR=1.831,P=0.010).MTHFR A1298 C ? MS A2756 G and MSR A66 G genetic polymorphisms and genetic models were not correlated with VTE.(9)There were significant differences between MTHFR C677 T and Hcy level before and after adjusting gender,age,ethnicity and smoking etc, P<0.05.(10)MTHFR 677 TT type and MSR 66 GG type of the Uygur existed at the same time increase the risk of VTE, OR=3.977,95%CI:1.033-15.585,P=0.002;MTHFR 677 TT type and smoking of the Uygur existedat the same time increase the risk of VTE,OR=4.223,95%CI:1.404-12.695,P=0.001; MTHFR 677 TT type and smoking of the Han existed at the same time increase the risk of VTE, OR=4.272,95%CI:1.798-10.153,P<0.001.(11)Compared with the control group, SAA levels of VTE groups were increased in Uygur and Han population, respectively(6.52±4.07 vs.5.45±2.79)mg/L and(8.29±5.33 vs.6.78±3.27)mg/L,but SAA level is not an independent risk factor for VTE in Uygur and Han nationality.(12)The distributions of rs12218 and rs4638289 gene polymorphisms in Uygur and Han nationalities do not exist among ethnic groups. rs12218 and rs4638289 gene polymorphisms are not risk factors for VTE. Conclusion:(1)Behavioral habits:smoking is a risk factor for VTE in Uygur and Han ethnic groups.(2)Diet: increased intake of red and processed meat is a independent risk factor of Uygur VTE,and increased intake of vegetables is the protective factor in VTE.(3)Hcy and CRP levels were elevated in VTE patients, Hcy is one of the common risk factors of Uygur and Han ethnic,while CRP is a risk factor for VTE in Han population.(4)MTHFR 677 TT type is an independent risk factor for VTE.The level of Hcy was higher in patients with type TT.It is suggested that the TT genotype and T allele may increase the plasma Hcy level and promote the development of thrombosis,which is one of the pathogenesis of VTE.(5)In Uygur population,MTHFR 677 TT and MSR 66 GG coexistence increased the risk of VTE;In Uygur and Han population, MTHFR 677 TT and smoking coexistence increased the risk of VTE,to help identify high-risk VTE groups.(6)Inflammation:the SAA level and its gene polymorphisms were not correlated with VTE,however,the further expansion of the sample size detection and analysis may have a new discovery. |
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