The Study Of LncRNA/mRNA Expression In Type 2 Diabetes Mellitus Within Kidney-yin Deficiency

ObjectiveSubject in the study are type 2 diabetes mellitus within kidney-yin deficiency, without kidney-yin deficiency. The study use lncRNAs microarray to screen out the different lncRNAs and mRNAs related to kidney-yin deficiency and type 2 diabetes mellitus. Meanwhile, the possible mechanism of the different lncRNAs in type 2 diabetes mellitus within kidney-yin deficiency would be discussed.Methos1. The lncRNAs and mRNAs microarray is used in the study in order to get the expression of mRNAs. The data is compared to obtain the different mRNAs. The data bases of GO and KEGG are used to analyse biological process, cellular component, molecular function and biological signal pathway about the different mRNAs.2. The lncRNAs and mRNAs microarray is used in the study in order to get the expression of lncRNAs. The data is compared to obtain the different lncRNAs.The target genes of different lncRNAs are predicted. Then, the target genes and different mRNAs are analysed together. The purpose is to find out lncRNAs which were related to kidney-yin deficiency and type 2 diabetes mellitus, and discussed the possible mechanism.Result1. Expression of mRNAs:In comparison of type 2 diabetes mellitus within kidney-yin deficiency and without kidney-yin deficiency, the 117 different mRNAs are found. Among these mRNAs, the expression of 37 mRNAs is up-regulated, and 80 mRNAs is down-regulated. GO annotations include the immune and stimulus response. KEGG annotations include 13 related pathways. In comparison of type 2 diabetes mellitus within kidney-yin deficiency and healthy, the 107 different mRNAs are found. Among these mRNAs, the expression of 88 mRNAs is up-regulated, and 19 mRNAs is down-regulated. GO annotations include the immune and stimulus response. KEGG annotations include 9 related pathways. In comparison of type 2 diabetes mellitus without kidney-yin deficiency and healthy, the 89 different mRNAs are found. Among these mRNAs, the expression 67 of mRNAs is up-regulated, and 22 mRNAs is down-regulated. GO annotations include the immune and stimulus response. KEGG annotations include 8 related pathways.2. Expression of lncRNAs:In comparison of type 2 diabetes mellitus within kidney-yin deficiency and without-yin kidney deficiency, the 208 different lncRNAs are found. Among these lncRNAs, the expression of 64 lncRNAs is up-regulated, and 144 lncRNAs is down-regulated.162 lncRNAs may have a role in cis-regulation, and 63 lncRNAs in trans-regulation. The target genes of 9 lncRNAs change in the different mRNAs. In comparison of type 2 diabetes mellitus within kidney-yin deficiency and healthy, the 275 different lncRNAs are found. Among these lncRNAs, the expression of 217 lncRNAs is up-regulated, and 58 lncRNAs is down-regulated.161 lncRNAs may have a role in cis-regulation, and 107 lncRNAs in trans-regulation. The target genes of 8 lncRNAs change in the different mRNAs. In comparison of type 2 diabetes mellitus without kidney-yin deficiency and healthy, the 309 different lncRNAs are found. Among these lncRNAs, the expression of 100 lncRNAs is up-regulated, and 209 lncRNAs is down-regulated.223 lncRNAs may have a role in cis-regulation, and 116 lncRNAs in trans-regulation. The target genes of 6 lncRNAs change in the different mRNAs.ConclusionThe occurrence and development of type 2 diabetes mellitus within kidney-yin deficiency and without kidney-yin deficiency are related to immune and stimulus response. Among its related pathyways of the occurrence and development of type 2 diabetes mellitus within kidney-yin deficiency are NF-kappa B signaling pathyway, MAPK signaling pathway, and AMPK signaling pathway. Among its related pathyways of the occurrence and development of type 2 diabetes mellitus without kidney-yin deficiency are NF-kappa B signaling pathyway, Wnt signaling pathway. In the occurrence of type 2 diabetes mellitus within kidney-yin deficiency and without kidney-yin deficiency, lncRNA AK125889 may play a regulatory role rested on the regulation of ability about anti-infection, and activity of NADPH oxidase. In the occurrence and development of type 2 diabetes mellitus within kidney-yin deficiency, lncRNA AF116618 may play a regulatory role rested on the regulation of cell proliferation and apoptosis, lncRNA AK126161 may play a regulatory role rested on the regulation of PKA signaling pathway, and lncRNA NR₀24151 may reduce the phagocytosis and clearance of immune complexes. Therefore, lncRNA AK125889?AF AF116618. AK126161?NR₀24151 may be the biomarkers and therapeutic targets of potential for type 2 diabetes mellitus within and without kidney-yin deficiency.