Correlation Of IL-17 Level In Synovia And Severity Of Knee Osteoarthritis

Part I:IL-17 expression and significance in osteoarthritis synovial fluidBackground:To investigate the expression of IL-17 in synovial fluid of knee osteoarthritis patients. IL-17 is associated specifically in osteoarthritis pathogenesis, exploring the role of IL-17 in the pathogenesis of osteoarthritis of the knee in, and provide theoretical and experimental basis for revealing the pathogenesis of knee osteoarthritis.Method:All patients were from March 2012 to February 2015 in our hospital orthopedics. In this study, samples from the two parts of knee osteoarthritis (Osteoarthritis, OA) group and normal control group. Knee OA group by imaging diagnosis and clinical standards and must row arthroscopic joint debridement total of 40 cases. While collecting 32 cases of traumatic meniscal or ligament damage caused by the fracture must row arthroscopic ligament repair or reconstruction of the normal control group of patients. There was no significant difference (P> 0.05) between the two groups in age, gender, body mass indices. During surgery on the joint fluid collected using a syringe to extract about 2-3 ml of joint fluid from the joint cavity, and injection tube which, immediately sent to the laboratory within half an hour after the synovial fluid collected and treated. Detection by enzyme-linked immunosorbent assay (enzyme-linked immunosorbent assay, ELISA), the content of the joint fluid IL-17 were measured. After completion of ELISA, the measured OD values, IL 17-concentration of the two groups are determined by the standard curve. Statistical analysis SPSS 17.0 statistical analysis software.Result:Normal control group, the content of knee joint fluid IL-17 was 2.17 ± 0.98 pg/ml, while in knee OA group IL-17 content was 6.37 ± 2.53 pg/ml, content and statistical analysis knee control group of IL-17 OA synovial fluid content difference in the comparison group was statistically significant (P<0.05). Knee OA synovial fluid level group of IL-17 was significantly higher than the control group.Conclusion:IL-17 plays an important role in the pathogenesis of knee osteoarthritis. Knee OA synovial fluid IL-17 expression was significantly increased, indicating that IL-17 is likely to participate in the pathogenesis of knee OA, causing joint destruction.Part ?:Correlation of IL-17 Level in Synovia and Severity of Knee OsteoarthritisBackground:The aim of this study was to evaluate the role of interleukin-17 (IL-17) level in synovia and its relationship with the severity of knee osteoarthritis (OA).Methods:We enrolled 226 OA patients and 106 controls in this study. The symptomatic/radiation severity of O A was assessed by the Western Ontario McMaster University Osteoarthritis Index (WOMAC) pain score/Kellgren-Lawrence (KL) grading system. Serum IL-17 levels were measured by enzyme-linked immunosorbent assay (ELISA).Results:Synovia IL-17 levels were significantly higher in OA patients compared with controls (P<0.01), and were negatively correlated with OA severity. IL-17 level gradually decreased among different phases but lacked statistical significance.Conclusions:IL-17 might play a crucial role in the pathogenesis of OA and is closely related to pain. Blocking the IL-17 signaling pathway may delay pain related to OA.