| This study proposed a new thought for quality improvement of cassava starch through regulating the components of plastid division and affecting amyloplast division in sink organs to change starch granule morphology, size and plumpness. Main research findings were as follows:accomplishing the clone of the full-length fragments of plastid division related ftsZ2 genes family in cassava, and predicting function and analyzing evolutionary relationships by using biological information science; preliminarily identifying the function of MeftsZ2 gene family with an endosymbiotic origin by making using of the serious influence of the abnormal expressions of FtsZ proteins on the features of Escherichia coli cell division; transforming cassava by constructing positive and negative plant carriers of constitutive and tuber specific promoters-driven MeftsZ2-1 genes, among which transformation of MeftsZ2-1 gene related carriers has obtained dozens of transgenic lines verified by molecular detection and successfully cultivated in the field, with a preliminary observation of the petiole and root tuber in transgenic plants presenting obviously blushing epidermal color as compared with wild type, and showing changes in phenotype corresponding to plastid development; additionally, observing starch granularity, amyloplast development and the occurring process of starch granules for multiple varieties, providing a theoretical basis for the new thought by applying this method to improve cassava starch quality. |
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