The Research Of HMGA1Expression In Colorectal Cancer And Effect Atfer Intervention Of Blister Beetle

CRC is a kind of malignant lesions of colorectal epithelium in a variety ofenvironmental or genetic factors. It is one of the common malignant lesions. Globalannual new cases of CRC are about100million people, accounting for10%of allmalignant tumors to15%.High expression of HMGA1can be induced malignant transformation of normalcells. The amount of HMGA1expression is biggest in embryonic development. Itsexpression reduced in fully differentiated mature cells or was not expressed. Thereaseach between S100A3and tumor was few compared to other members of thefamily of calcium-binding S100A.The affinity of S100A3can be considered as azinc ion signaling proteins. S100A3expression may be involved in malignanttransformation in the absence of early breast cells. Whether S100A3and HMGA1play an important role in CRC?Cantharides are commonly known as Meloidae spot lytta insects. Cantharidinatewas a special effect for primary liver cancer. Cantharidinate was used more and morewidely. It was also gradually in-depth study. In recent years, study of cantharidinatemainly focused on the synthesis, biological functions, toxicity and physiologicalaspects in the United States and European countries. Anticancer mechanisms,pharmacology and applied research of cantharidinate were more in our country. Butthe cantharidinate for prevention and treatment of CRC had not been reported.This study investigated the preventive and therapeutic effects of cantharidinate onhuman colorectal cancer both in vivo and in vitro. In addition, the mechanismsinvolved in origination and development of renal interstitial fibrosis also beenapproached. The main research contents is composed ofâ‘ the relationship ofHMGA1and S100A3may be linked and investigated the role of HMGA1and S100A3in human CRCâ‘¡the role of cantharidinate are observed for CRCâ‘¢Theinhibition role and effect of cantharidinate are investigated for HMGA1and S100A3gene in human colorectal cancer.â‘£The effect of cantharidinate is analyzed byRaman spectra.Main results were as follows:1. The protein expression of HMGA1and S100A3was significantly increasedin cancer tissues compared with the adjacent tissues.2. The expression of S100A3and HMGA1may be correlated in CRC.3. Immunohistochemistry and Western blot results confirmed that theexpression of HMGA1and S100A3was significantly increased in cancer tissuecompared with adjacent tissues (P <0.05and P <0.01).4. After intervention of cantharidinate, Cell cycle occurs more obvious changesin colorectal cancer.The expression of HMGA1and S100A3decreased gradually(P<0.05). Its protein structure also improved after intervention of cantharidinatethrough Raman spectra.Conclusions were as follows:1. S100A3and HMGA1play an important role in colorectal cancer.2. Cantharidinate is similar with chemotherapy, it can reduce the gene andprotein expression of S100A3and HMGA1in tumor cells and improve cycle.