The Effect Of Jingtong Yushu On The Expression Of Endometrial NF-KB In Endometriosis Rats

Object i ve:1To study the pathologic changes of the transplanted endometriotic tissues of the endometriosis rat model established by the method of autoplastic transplantation, to decide the best dosing time of prevention and therapy.2To study the effect of "Jingtongyushu" on the pathologic changes as well as the protein and RNA expression of NF-κB in the transplanted endometriotic tissues of the endometriosis rat model, providing experimental foundation for using "Jingtongyushu" to remedy endometriosis.3To study the effect of "Jingtongyushu" on the pathologic changes and the expression of NF-κB in the transplanted endometriotic tissues of the endometriosis rat model with preventive and therapy dosing separately, discussing the therapeutic mechanism of preventive dosing of "Jingtongyushu".Methods:1In the first part of animal research, female rat without offspring were divided into model3days group, model5days group, model4weeks group, model6weeks group and model8weeks group. There were4rats in each group. They were executed at3days,5days,4weeks,6weeks and8weeks after became endometriosis model established by the method of autoplastic transplantation. Then the pathologic changes of transplanted endometriotic tissues were compared with normal rats by the method of HE stain.2In the second part of animal research,72endometriosis rats were divided into model4weeks group, model8weeks group,"Jingtongyushu" group (low-,medium-,high-dosage) and danazol group, using female rats without offspring as control group. There were12rats in each group. The4weeks group were executed after4weeks, other groups were executed after8weeks. The changes of transplanted endometriotic tissues, as well as the protein and RNA expressions of NF-κB were detected by HE stain, Immunohistochemical stain, Western blot and RT-PCR.3In the third part of animal research,36endometriosis rats were divided into model group, preventive group and therapeutic group, using female rats without offspring as control group. There were12rats in each group. They were executed after8weeks, The changes of transplanted endometriotic tissues, as well as the protein and RNA expressions of NF-κB were detected by HE stain, Immunohistochemical stain, Western blot and RT-PCR.Results:1Endometriosis model were established satisfactorily by the method of autoplastic transplantation, the success rate was70percent. Model3days and model5days group didn’t show mature transplanted endometriotic tissues, but model4weeks,6weeks and8weeks groups had already builds mature transplanted endometriotic tissues.2"Jingtongyushu" high dosage group and danazol group effectively reduced the number of the endometrial stromal cells, and shrunk transplanted endometriotic tissues.3The Immunohistochemical stain in first part showed that, compared with control group, NF-κB expression significantly increased in model groups,"Jingtongyushu" groups and danazol group. Compared with model8weeks group,"Jingtongyushu" high-dosage group and danazol group significantly decreased NF-κB expression, and "tongjingtong" high-dosage group had better effectiveness.4The Western blot in first part showed that, compared with control group, the protein expression of NF-κB significantly increased in model groups,"Jingtongyushu" groups and danazol group. Compared with model8weeks group,"Jingtongyushu" high dosage group and danazol group significantly decreased protein expression, and "Jingtongyushu" medium,high-dosage groups had better effectiveness.5The RT-PCR in first part showed that, compared with control group, the RNA expression of NF-κB significantly increased in model groups,"Jingtongyushu" groups and danazol group. The effect of "Jingtongyushu" and danazol groups was similar with the results of Western blot.6In the third part of animal research,"Jingtongyushu" preventive and therapeutic groups had successfully shrink transplanted endometriotic tissues, the preventive group had better effectiveness.7The Immunohistochemical stain in third part showed that, compared with control group, NF-κB expression significantly increased in model group, preventive and therapeutic groups. Compared with model group, preventive group and therapeutic group significantly decreased NF-κB expression, but there were no difference in these two groups.8The Western blot showed that, compared with control group, the protein expression of NF-κB significantly increased in model group, preventive and therapeutic groups. Compared with model group, preventive group and therapeutic group significantly decreased the protein expression of NF-κB, but there were no difference in these two groups.9The RT-PCR showed that, compared with control group, the RNA expression of NF-κB significantly increased in model group, preventive and therapeutic groups. The effect of preventive and therapeutic groups was similar with the results of Western blot.Conclusion:1Endometriosis model can be established successfully with the method of autoplastic transplantation. Choosing5days and4weeks after model built as the preventive, therapeutic dosing time.2"Jingtongyushu" can shrink the transplanted endometriotic tissues of endometriosis model by decreasing its protein and RNA expressions of NF-κB.3Preventive using of " Jingtongyushu" can also shrink the transplanted endometriotic tissues of endometriosis model, as well as decrease the protein and RNA expressions of NF-κB, but there was no difference between preventive and therapeutic groups.