| [Objective]Observe the effect of Tuina therapy to nerve functional recovery of SNI rats through behavioristics, morphology. Then probe the biological mechanisms of Tuina therapy in SNI through the content of NT-3, TrkC in spinal cord and DRG.[Methods]Use SD rats as experimental animals and made the SNI model by nerve clamping method. Rats were divided into5groups. Then rats were treated by manipulation simulator at BL37, BL57and GB34. After treatment, we first tested PWL, SFI, BBB score to evaluated the recovery of sensory and movement function. Then observed morphologic changes in spinal cord, sciatic nerve and gastrocnemius and search the evidence. Finally, detected the content of NT-3, TrkC in spinal cord and DRG to interpreted the mechanism of Tuina to promote nerve regeneration and repair.[Results]1Results of behavioristicsResults of BBB score:7days after operation, the score of the model group were significantly lower than the blank control group and sham-operated group (p <0.05). After10and20treatments, scores of Tuina group, model group and model control group were all higher than before and the score of Tuina group was the highest, which higher than model group and model control group, but still significantly lower than blank control group (p<0.05).Results of SFI:7days after operation, the score of the model group were significantly lower than the blank control group and sham-operated group (p<0.05). After10and20treatments, scores of Tuina group, model group and model control group were still much lower than the other two groups (p>0.05).Results of the PWL test scores:compared the PWL test scores of left foot between each group, no statistical difference. The result of the right foot had statistical difference.7days after operation, the score of the model group was significantly higher than the blank control group and sham-operated group. Indicate that the continuity of nerve fibers had been damaged by operation. The score of Tuina group was significantly lower than model group and model control group after10and20treatments (p<0.05), while no statistical differences compared with normal group (p>0.05). Indicate that Tuina therapy could promote the sensory recovery of SNI rats.2Results of morphologyParts of the neuron in spinal cord of model group dead7days after operation, while some of their cell body swelled etc. Degeneration, demyelination, swell and rupture had been observed in injured nerve. After10and20treatments, pathological changes of model group, model control group and Tuina group were all better than before. The recovery of Tuina group was better. Meanwhile, the diameter of affected gastrocnemius cells of Tuina group was bigger than model group and model control group.Numbers of myelinated nerve fiber of model group was much lower than model group and sham-operated group7days after operation (p<0.05). After10treatments, numbers of myelinated nerve fiber of model group, model control group and Tuina group were higher than before, but still much lower than blank control group (p<0.05). Meanwhile, numbers of model group and model control group were all lower than Tuina group (p<0.05). Result after20treatments is similar to10treatments, and numbers of model group, model control group and Tuina group all increased, numbers of Tuina group increased more.3Results of the NT-3, TrkCIn the spinal cord ventral horn, the NT-3level of model group was higher than blank control group (p<0.05). Meanwhile, the NT-3level of Tuina group continued to rises after10and20treatments and all higher than other four groups (p<0.05). The NT-3levels of model group was increased after20treatments, higher than blank control group but lower than Tuina group (p<0.05).Results of the NT-3in DRG were similar to results in the spinal cord ventral horn.In the spinal cord ventral horn, the TrkC level of model group was higher than blank control group (p<0.05). After10and20treatments, the TrkC levels of model group, model control group and Tuina group were all higher than other two groups (p<0.05). Meanwhile, level of Tuina group was higher than model group and model control group.In DRG, the TrkC level of model group was higher than blank control group7days after operation (p<0.05). After10and20treatments, the TrkC level of Tuina group was higher than other groups (p<0.05).[Conclusion]1Tuina therapy could promote the PWL scores and BBB scores of the SNI rats which indicate that Tuina is effective to improve the sensory and movement function.2Tuina therapy could increase the number of myelinated nerve fiber, rebuild connection between damage nerve axons and the target organ, then promote the recovery of nerve function.3Tuina therapy could increase the level of NT-3and TrkC in spinal cord and DRG, then activated the combination of NT-3and TrkC, finally nerve regeneration started and neurons were protected. This could be a mechanism of Tuina therapy of promoting nerve injury repair. |
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