Mechanism To Improve Invigorating Kidney Endometrial Receptivity Molecular Role

Background:Infertility is the frequently-occurring disease and venereology women of childbearing age, assisted reproductive technology brought hope for infertile couples. At present the main bottleneck restricting the development of ART is to the receptivity of endometrium fall in clinical pregnancy rate hovering at a low level. For this situation, we in the traditional Chinese medicine under the guidance of the classical theory of "kidney main reproductive", therapy of traditional Chinese medicine to improve the clinical curative effect of endometrium and the receptivity of endometrium was accepted gradually. Methods:This topic through the animal experiment, cell experiments and clinical trials, the objective evaluation of kidney qi and improve curative effect and the receptivity of endometrium, on the basis of systematic analysis of its internal molecular mechanism and evaluate its clinical efficacy. By copying the rat super stimulate ovulation models to kidney invigorate the circulation of the intervention unfertilized and pregnancy rats respectively, analysis of endometrial morphology, blood vessels, and the receptivity of the uterus molecular markers of change; Cell experiments, based on uNK cells and endometrial cells paracrine system, application of gene chip technology research clear kidney invigorate the circulation of Chinese traditional medicine side effect in the physiological process. Results:Kidney and activating blood can improve the unfertilized super stimulate ovulation in rats model of endometrial MVD and expression of LIF; Kidney invigorate the circulation of the Chinese traditional medicine can adjust the MVD of the film inside the uterus of rats around the time of planting and angiogenesis factors related to the expression of VEGF and OPN; UNK cells of uterine epithelial cells and stromal cells uterus/epithelial cells co-culture system in paracrine effect can promote the proliferation of uNK cells themselves, at the same time promote the receptivity of endometrium and guarantee the stability of early pregnancy, and support Chinese medicine kidney invigorate the party in the physiological process. Conclusions:Chinese medicine kidney invigorate the endometrial receptive, perhaps by uNK paracrine effect to improving uterine microenvironment in angiogenesis.