The Research Of MiRNA Expression Of Kidney Deficiency Syndrome Of T2DM Based On The "The Kidney Is The Congenital Foundation" Theory

Objectives:Diabetes has become one of the most important threats to people especially Type2Diabets (T2DM) in our country. The mechanism of T2DM has been proved with complex genetic background and environmental factors. Basd on the traditional Chinese medicine theory "the kidney is the congenital foundation", the study of T2DM mechanism can better reveal the interaction of "gene-environment-disease" and the whole concept of Chinese medicine. In this paper, we tried to explore the epigenetic mechanism in kidney deficiency syndrome of T2DM.1. To discuss the innovation and application value of combination of epigenetics method and Chinese medicine phenomenological research in theory by summary the ancient Chinese medicine book and epigenetic methods.2. To reveal the influence of genetic factors on kidney deficiency through the large scale investigation with kidney deficiency scale.3. To explore the mechanism of kidney deficiency syndrome of diabetes from the molecular level by miRNA microarray.Methods:1. The combination of disease and syndrome:Clinical investigations with kidney deficiency facto scaling on T2DM patients. And the then statistic analyzed the distribution characteristics of kidney deficiency syndrome in T2DM2. miRNA microarray tests:miRNA microarrays screen out potential differentially miRNA for type2diabetes with kidney-deficiency group, non-kidney-deficiency group and normal group. The target gene predicted for the key miRNA.3. Fluorescent quantitation PCR:candidate characristic miRNA in "kidney deficiency group-non kidney deficiency group"were tested with PCR.Results:1. Based on ancient Chinese literature, kidney deficiency is thought to be one of the most important factors in the pathogenesis of diabetes. With epigenetics techology, kidney deficiency syndrome in microscopic nature rich type2diabetes can be enriched.2. In793T2DM patients, there are169cases of patients with family history, accounting for21.3%. There is no dfference in male and femal.The percentage of kidney deficiency patients with a family history was significantly higher than that of no family history (P<0.05). That suggested "deficiency of kidney" may play a role in the familial aggregation of T2DM.3. There are13different miRNA between kidney deficiency group and non-kidney deficiency group; there are12different miRNA between kidney deficiency group and normal controls; there are11different miRNA between non kidney deficiency group and normal controls.4.3candidate different miRNA (miRNA:miR-335.miR-377and miR-497) between kidney deficiency group and non kidney deficiency group were chosen to qPCR verification. The results were consistent with the microarray results. However, the difference between groups was not significant (P>0.05).Conclusions:1. With the help of Epigenetics Chinese medicine research such as Chinese medicine syndromes and genetics may achieve a breakthrough. At the same time the theory "the kidney is the congenital foundation" can be better explained.2. By the investigation of family histories of T2DM patients, the congenital kidney deficiency may play a role in the pathogenesis of T2DM.3. The miRNA basis of kidney deficiency syndrome in T2DM related to the immunity and the aging which may imply kidney deficiency in T2DM possible mechanism to reduce cell function. 4. The miRNA microarray and candidate miRNA qPCR results are basically consistent. It may imply that T2DM deficiency syndrome has certain significance in miRNA level changes which should be necessary to explore deep later.