The Study Of The Functional Effects And Mechanisms Of Elemene On Breast Cancer Cell Lines MDA-MB-231and MCF-7and Endothelial Cell Line HECV

Objective: The aim of this study is to test whether Elemene has the ability to impactthe functions of breast cancer cell lines of MDA-MB-231and MCF-7and endothelial cellline HECV, and to find the mechanism.Methods: After treated with Elemene as the treatment group and compared withcontrol group, the growth rates, adhesion abilities, migration abilities and invasion abilitiesof MDA-MB-231and MCF-7cells were successively detected by Crystal violetexperiment, ECIS assay and Invasion assay.After treated with Elemene as the treatment group and compared with control group,the growth rates, adhesion abilities, migration abilities, tubule formation and cellaggregation of HECV cells were successively detected by Crystal violet experiment, ECISassay, tubule formation assay and cell aggregation assay.MDA-MB-231cells were treated by Elemene at a concentration of40μg/mL for48hours. Morphological changes were observed by Inverted microscope, and Caspase-3,-8,-9RNA levels were investigated by Q-PCR.Results: When Elemene reached on a certain concentration, the growth rates ofMDA-MB-231(≥20μg/mL, P<0.05) and MCF-7(=160μg/mL, P<0.05) cells wereinhibited, and had a dose-dependent. As the increasing of the concentration of Elemene,the adhesion ability of MDA-MB-231cells (≥80μg/mL, P<0.05) and MCF-7cells(=160μg/mL, P<0.05) were down-regulated; The migration abilities of MDA-MB-231cells were reduced (≥40μg/mL, P<0.05), while the changes of MCF-7cells showed no statisticaldifference; The invasion ability of MCF-7cells were impacted by Elemene (=160μg/mL,P<0.05), but the same phenomenon did not appear on MDA-MB-231cells.Elemene at a low concentration has the ability to enhance HECV cells on growth(=20μg/mL, P<0.05), adhesion (=10μg/mL, P<0.05), migration (=10μg/mL, P<0.05) andtubule formation (=20μg/mL, P<0.05), while the high concentration of Elemene inhibitsthe functions of growth (≥80μg/mL, P<0.05), adhesion (≥80μg/mL, P<0.05), migration(≥80μg/mL, P<0.05), tubule formation (≥80μg/mL, P<0.05) and cell aggregation(≥80μg/mL, P<0.05).Cells were changed to an apoptotic status under the microscope, and the expressionlevels of Caspase (Caspase3:56.7±15.7vs.812.2±222.6, P=0.025; Caspase8:140.3±25.6vs.327.8±50.1, P=0.032; Caspase9:13.1±4.4vs.5.7±1.5, P=0.623) of MDA-MB-231cellswere upgraded after treated with Elemene at a concentration of40μg/mL.Conclusion: Elemene has the capability to inhibit the growth rates, adhesion ability,migration ability and invasion ability of breast cancer cells. While for endothelial cells,Elemene has the capability to enhance cell functions at a low concentration, but inhibits thefunctions at high concentration. Elemene has the capability to induct apoptosis of breastcancer cell line MDA-MB-231.