Study On The Determination Methods For Some Plant Hormones And Drugs And Their Interaction With Biological Macromolecules

Establishing supertrace and in situ determination method for plant hormones havegreat significance in the study of plant hormones and its molecular mechanism.Thereaseach of quality standards for drugs and drug kinetics is an important part of druganalysis. In this thesis, high-sensitivity determination methods such aschemiluminescence, electrochemiluminescence（ECL） and fluorescence method wereestablished for some plant hormones and drugs analysis, and the interactions betweenthese substances and biological macromolecules had been studied also.The thesis is divided into nine chapters. The first chapter introduces thecommonly used chemiluminescence and ECL system and some new technologyapplications in drug analysis. The commonly methods used for studying the interactionsbetween protein and small molecule were introduced and the progress in cytokinindetection were reviewed. Furthermore, the purpose，contents and significance ofthe thesis were summed up.Chemiluminescence and electrochemiluminescence detection method had beenapplied in chapter2-7. In Chapter2, based on the facts that some of cytokinins canenhance the ECL intensities of Ru（bpy）32+, a sensitive ECL method for cytokinins hasbeen proposed, the assay conditions of cytokinins electrochemiluminescence wereoptimized, and the possible mechanisms had also been discussed. In Chapter3, the ECLassay method for Kinetin was established base on the facts that Kinetin can inhibit theECL of CdS modified glassy carbon electrode-potassium persulfate system, the thedetection limit was as low as4.2×10^-10mol/L, the methods has some significance forin situ detection. In Chapter4, Glucose oxidase（GOD） was encapsulated in theGraphene/Nafion film modified glassy carbon electrode（GCE） and used as an ECLsensor for glucose.The GOD retains its bioactivity after being immobilized into thecomposite film. The sensor gives a good responds to glucose with a linear response inthe range of2.0×10-6¹.0×10-4mol/L and with a detection limit of1.0×10-6mol/L. InChapter5, a capillary electrophoresis-ECL method had been developed for famciclovir assay, and which had been applied to determine the compound of todeterminatefamciclovir in capsule, the possible mechanism was studied also. In Chapter6, a newflow injection chemiluminescence method for meloxicam determination had beenproposed, the detection limit is3.7×10^-10g/mL, and the method was apply to thedetermination of meloxicam tablet.Fluorescence analysis method had been applied in Chapter7～9.In Chapter7,based on the fact that the hydrolysis reaction product of meloxicam with sulfuric acidhas strong fluorescence properties, a synchronous fluorescence analysis method for thedetermination of meloxicam in tablets was established, the detection limit was1.9×10^-9mol/L. In Chapter8, the fluorescence quenching effect of tamibarotin to bovineserum albumin had been applied to analysis the quenching types, interaction force,binding sites number, binding constant and the conformational changes whentamibarotin interact with bovine serum albumin.A fluorescence quenching method forthe analysis of tamibarotin in simulated samples was also established. In Chapter9,fluorescence spectroscopy and UV spectra was used to analysis the interaction betweenfour cytokinins and bovine serum albumin, casein, soybean protein, the study providesthe theretical basis for molecular mechanism of cytokinin and pharmacokinetics.