Effect Of Different Factors On Chemical Ingredients Of Medicinal Plants Including Salvia Miltiorrhiza

The application of traditional Chinese medicine in china has a long history. It takes anirreplaceable role in the disease prevention and treatment of some common diseases, chronicdiseases. But recent years, with the changes of environmental factors, traditional Chinesemedicine has seriously degraded, the quality of which directly affects the clinical treatment.The merits of the traditional Chinese medicine efficacy has a close related with its chemicalcomposition of the active substances-medicinal ingredients, therefore investigating theinfluence of environmental factors on the growth of medicinal plants and correlation analysisof their medicinal ingredients can help us scientific planting medicinal materials production, itcan provide theoretical reference for improving the clinical curative effect of traditionalChinese medicine. The research of this article focus on the effect of two different conditionsof medicinal plant related factors on the main medicinal ingredients: flavonoids in Flaveriabidentis which has a good future potential development (natural environment)、tanshinone andphenolic acid in traditional medicine Salvia miltiorrhiza plants (Potted plant environment),the main results as follows：1、Under the conditions of this study, establishing a method of extraction and separationof flavonoids which is the main components in Flaveria bidentis, the optimum conditions:80%ethanol, Material liquid than1:20(w/v), Backflow75minutes at a time, Back2times. F.bidentis powder extract yield can reach17.62%. Chromatographic conditions: Apollo C18Column (150mm×4.6mm), Methanol solution for mobile phase A, B for φ=0.05%phosphoric acid aqueous solution, A gradient elution (0~8min, φ=65%B→φ=55%B;8~25min, φ=55%B→φ=50%B;25~30min, φ=50%B→φ=45%B;30~50min, φ=45%B;50.01~55.01min, φ=65%B), a flow rate of1.0ml/min, detection wavelength at360nm, column temperature is10℃.2、The same composition of Flaveria bidentis are differences among different plots. Ittheoretically verified authentic ingredients attention geographical argument. For this situation,the difference may be caused by environmental factors such as soil factors.3、The soil factors of F.bidentis of and the blank plots have significant difference. Wheresoil pH, available P, alkali solution N and urease increased, but soil alkaline phosphatase, acidphosphatase and organic carbon decreased. The results suggest that the mechanism of inhibition of the growth of other plants might be its strong capacity of adsorption which couldmake the land barren.4. The study found a correlation between soil factors and F.bidentis’s main medicinalingredient flavonoids. There was a positive correlation among soil available P, quercetinglycosides, isorhamnetin sulfate and AST. The correlation coefficients were0.701*0.670*,0.681*, respectively. The significantly negative correlation was found among soil alkalinephosphatase, marigold glucosides and6-methoxy kaempferol glycosides. The correlationcoefficient were-0.740*,-0.777*, respectively. Thus, soil available P and alkaline phosphatasehad a distinct role in the accumulation of main component of F.bidentis.5. The study found that the growth and nutrient absorption of the Salvia miltiorrhizacould be adjusted by arbuscular mycorrhizal fungi, but the effect of arbuscular mycorrhizalfungal inoculation was influenced by soil water and nitrogen fertilizer. Taking into all thesefactors, the growth effect was best under70%of soil water, N0.16g/kg.6. AM inoculation can improve the content of K, Ca and other elements, which promotethe growth of Salvia miltiorrhiza under the same soil water and nitrogen fertilizer conditions.AM inoculation can improve the survival ability of Salvia miltiorrhiza adversity underdifferent soil water conditions. The content of medicinal components of Salvia miltiorrhizaare influenced by nitrogen fertilizer. When the amount of nitrogen is higher than a certainvalue, the content of medicinal components of Salvia miltiorrhiza as a proportion of the totalfalls.This paper examines the relationship between the different conditions of flavonoids inthe Flaveria bidentis, and the effects of growth and medicinal components of AM inoculationof Salvia miltiorrhiza under different soil water and nitrogen fertilizer conditions It provides atheoretical basis in improving the quality of medicinal plants.