Association Of MiRNA-203with SOCS3and The Mechamisms In HaCaT

microRNAs are recently discovered, small, non—coding, single-strand RNAs, which are small, approximately22nucleotides (nt). It is like siRNA. miRNAs are binded to the3’untranslated regions (UTR) of target mRNAs in a sequence specific manner in order to influence the translation or stability of the transcripts. They act as the total" switch" of disease development, involved in cell proliferation, apoptosis, differentiation, metabolism, development, tumor metastasis and other biological processes. About1048miRNAs involved in the regulation of the human genome. Therefore, the role of microRNAs, in particular, the identification and study of its target genes, is one of the major hot spots in the present study.miR-203is one of non-coding miRNAs which have close relation with differentiation of the stratified epithelial tissues.miR-203is significantly up regulated in psoriasis, At the same time, the decline in the expression of SOCS3expression is increased in the psoriatic epidermis. A large number of studies have shown that in tumors: miR-203has a role in inhibition of cell proliferation, and can thus play an important role in the development of the disease in conjunction with specific target molecules.Therefore, this study was performed to investigate the effect of microRNA-203(miR-203) on cytokine signaling3(SOCS3), and the association between both factors and the role of acitretin in HaCaT cell. Methods and result:1. Based on bioinformatics online software and database comparison found that the The SOCS3gene3’UTR area located in the miR-203binding "seed" sequence, thus changing the level of gene expression can be combined with each other.2. we observed the situation of miR-203and SOCS3binding by fluorescence hormone reporter gene analysis method, exogenous to give ’mimic miRNA-203’sequence containing the SOCS3gene3’UTR of the luciferase reporter gene vectors were co-transfected into HEK293T cells. This experiments confirmed binding of miR-203and SOCS3, affecting the activity of the luciferase reporter gene.3. In this study, We found miR-203is able to decrease endogenous SOCS3expression using western blot. Moreover, we have demonstrated that SOCS3is a novel target of miR-203by luciferase reporter assay.4. we observed the impact of inhibitor miR-203can impact STAT3signaling pathway by Western blot.5. using the cell-flow method. We found that miRNA-203mimetic agent to increase in the S phase of the cell, thus affecting its proliferation.6. To learn more about miRNA-203in the treatment of psoriasis. We use cell flow cytometry to observe the cell cycle. We found Acitretin can increase the S-phase cells, slows down cell proliferation, but this effect was reversed by the inhibitor miR-203.Conclusions:1. SOCS3may be a potential target gene of miR-203;2. mimic miR-203inhibit SOCS3, and active STAT3signaling pathway;3. Acitretin can infect the cell cycle of HaCaT cells reversed this infection can be miR-203of inhibitors;...