| Background:A novel retrovirus XMRV has been identified in the prostatic tissue of prostate cancer patients and was thought to have close relationship with prostate cancer. However, there are still some controversial results about the relationship of XMRV and prostate cancer, some negative results in prostate cancer were reported since then, some researchers consider that the false-positive results in assays for XMRV were caused by laboratory-based contamination of mouse endogenous viral sequence.Recently, the number of patients with prostate cancer who needed to be treated with radical prostatectomy increased rapidly in China. There is still a difference between clinical staging and the post-operative final pathologic staging; hence, an excellent tool for accurately predicting the pathologic stages of prostate cancer is needed urgently in clinical practice. The Partin tables are the most popular and widely used tool for predicting the pathologic stages of prostate cancer because of its high accuracy and ease of implementation. Material and methods:189cases prostate samples (including both blood sample and tissue sample) were taken from the Department of Urology in The second affiliated hospital of Zhejiang University from March2009to September2012.251cases of nomal healthy samples were collected as control group. All blood samples were deposited in-40refrigerator, tissue samples were deposted in-800rifregerator.Nest PCR were used to detected the XMRV’s gag leader region, env and gag sequence in both prostate samples and control group samples, the plasma of positive samples were co-cultured with with LNCap cell strain, then the LNCap cell were tested by PCR and Western Blot for XMRV, the PBMCs of positive samples also were tested by PCR to detect XMRV. The intracisteral A partical (IAP) sequences was used to detect the mouse endogenous viral sequence in positive samples, try to clarify if there exist the laboratory-based contamination in our study.The clinical data of156cases who underwent the radical prostatectomies in our department between June2000and May2012. were retrospectively analyzed The accuracies of the three versions of the Partin tables in predicting the post-operative pathologic stages in Chinese patients with prostate cancer were evaluated using the area under the receiver operating characteristic curve (AUC).Results:R462Q variant was20.2%in Chinese.5cases prostatic samples and10cases samples in control group were found to contain the XMRV’s5’gag leader region, gag or env sequences, but failed to detect the whole XMRV sequences in all samples. The results were negative in all positive samples which were co-cultured with LNCap cell strain which tested by Western Blot and PCR, and the results were also negative in PBMCs of these positive samples which were tested by PCR.3cases of total15positive sapmles were found to contain the mouse endogenous viral sequences, and was thought to be the result of laboratory-based contamination.Using the1997,2001, and2007Partin tables for predicting the current cases, the AUC of organ confinement (OC) was0.877,0.788, and0.726; the AUC of extracapsular extension (ECE) was0.525,0.615, and0.608; the AUC of seminal vesicle invasion (SVI) was0.875,0.649, and0.820; and the AUC of pelvic lymph node invasion (LNI) was0.808,0.758, and0.735respectively.Conclusion:there no definite evidence to indicate that XMRV can infect the prostate cancer of chinese patients except some MLV-like viruses. MLV-like mouse virus has no relationship with prostate cancer. The possibility of false-positive results which caused by laboratory-based contamination do exsit in XMRV research.The accuracies of the three versions of Partin tables in predicting OC, SVI, and LNI were good, especially the2001Partin table for SVI. In contrast, the accuracy of the three versions of the Partin tables in predicting ECE was fair. The1997Partin table was much better than the2007table in predicting OC, and the2001table in predicting SVI. The2007Partin table did not show any advantages. |
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