And Integration Frequency Of Cervical Cancer And Human Papillomavirus Infection And 16 Subtypes Load

Objective:To investigate HPV infection in cervical cancer and its precancerous lesions. To analyze the association between viral load, genomic integration frequency of HPV16and cervical carcinogenesis.Method:HPV infection and genotype were detected by PCR amplification and DNA sequencing in337cervical scrape samples. The copy number of E2, E6and β-actin were quantified to evaluate the viral load and integration status by real time PCR in40HPV-16single infection cases.Result:(1).The prevalence of HPV infection was higher in cervical cancer and precancerous groups than that in normal group, HPV16,58and18were the3most important high-risk HPVs.(2). The viral load was different among groups, which was higher in cancer group than that in HSIL group.(3). The median number of E2/E6was0.93in normal group,0.84in precancerous group,0.64in cancer group respectively, showing a descending trend with the progression of cervical disease.0.76was set as cutoff value to distinguish episomal form from mixed form. The proportion of episomal form decreased, while that of integrated form (mixed and pure integrated) increased with the disease progression.Conclusion:(1). The viral load in cancer group decreased, compared to that in HSIL group.(2). The positive correlation between HPV16genomic integration frequency and cervical neoplastic progression was confirmed.