| Objective:(1) To establish a zebrafish model for the research of inner ear hair cell damage and detect the expression of Gfi1.2in each stage of zebrafish inner ear development.(2)To find the mechanism of the injury to hair cells caused by ototoxic drugs.Materials and Methods:(1)Choose the zebrafish embryoes of4.3hpf、12hpf、24hpf、48hpf, useing the in situ hybridization to evaluate the expreeion of Gfi1.2.(2)Incubate the embryoes of48hpf with cisplatin of0.25mM、0.50mmM、0.75mM、1.OOmM、1.50mM for8hours, useing the In situ hybridization to evaluate the expreeion of Gfi1.2of the different embryoes.(3)Incubate the embryoes of48hpf with cisplatin of1.00mM for4h、8h、12h, useing the In situ hybridization to evaluate the expreeion of Gfi1.2of the different embryoes.Results:(1)The expression of Gfi1.2was detected at each stage of the embryoes. Strong expression was detected in the inner ear of the48hpf embryoes.(2)Ihe larger dose the embryoes were treated with cisplatin, the weaker the expression of Gfi1.2was.(3)the longer time the embryoes were treated with cisplatin, the weaker the expression of Gfi1.2was.conclusions:(1)Zebrafish a new model for the research of the embryonic development of the vertebrates because of its short developmental cycle, Relatively inexpensive price, transparent embryos and so on. It becomes one of the best model animals for researching the development of inner ear and deafness genes. It is shown in this experiment that it is feasible to use zebrafish as a animal model to access the damage of the hair cells induced by drugs.(2) Gfil is a transcriptional repressor. It has beeen shown that it plays an important role in the development of inner ear hair cells in mice with continued expression. This experiment showed that Gfi1.2(homologous gene of Gfil) also expressed itself in the inner ear of zebrafish and could be used as a marker of inner cell damage.(3)The hair cell damage caused by cisplatin is dose-dependent and time-dependent. |
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