Study Of The Effect Of Decorin On Liver Fibrosis Formation And Liver Regeneration Following Partial Hepatectomy Of Fibrotic Liver In Mice

PART ONEStudy of the effect of decorin on liver fibrosis in miceObjective To establish the liver fibrosis model of mice with CC14. Inject decorin (DCN) via caudal vein to the model, analyse the expressions of genes and proteins such as TGF-β1, a-SMA, TNF-a, HGF and Caspase3in liver tissues to investigate whether DCN can inhibite the process of liver fibrosis in mice or not.Methods (1) Liver fibrosis model was established by injecting CCl4into20mice (5-week-old, Balb/c) intraperitoneally once every two days on each mouse (1ml/kg, CCl4:olive oil=1:1), analyse the expressions of related proteins in liver fibrotic mice.(2) Three weeks after injecting CCl4intraperitoneally, mice were randomly devided into two groups:DCN group and control group. We injected DCN into DCN group mice twice a day at250ug/kg, and injected equal amount of PBS and NS into control group mice. Two weeks later, all the mice were sacrificed and their liver tissues were analysed by Real time PCR, HE staining, Masson Staining and immunohistochemical staining in the two groups. Results Exogenous protein DCN could reduce the liver fibrosis induced by CCl4in mice. The fibrosis degree of experimental group was alleviated, content of collagen fiber was lower in experimental group than that of control group. in addition, expressions of TGF-β and α-SMA decreased in experimental group.Conclusion Taking liver fibrosis model of mouse as the experimental subjects, by injecting extrinsic protein DCN to the model, we confirmed that DCN could inhibite the expressions of proteins related to fibrosis and reduce the formation of liver fibrosis of mice.PART TWOStudy of the effect of decorin on liver regeneration following partial hepatectomy of fibrotic liver in miceObjective To establish model of70%partial hepatectomy on mice of liver fibrosis. Then exogenous protein DCN was injected via caudal vein of these mice to investigate whether DCN could promote the regenration of liver of fibrosis after hepatectomy or not.Methods40mice (5-week-old, Balb/c) were injected CCl4, and6mcie were injected olive oil intraperitoneally. liver fibrosis model was established after5weeks. The survival mice were randomly devided into three groups:experimental group (DCN group), control group and liver fibrosis group, then we perfomed70%partial hepatectomy on all these mice and injected DCN or PBS plus NS to each group respectively after surgery. Hepatocyte proliferation index, Liver body weight ratio (LBR), RT-PCR and Immunohistochemistry were used to analyse liver regeneration of both groups, to find out whether exogenous protein DCN could promote the regenration of fibrosis liver after hepatectomy or not. Results Expressions of TGF-β1and α-SMA decreased in experimental group, LBR was higher in experimental group (P<0.05), and the expressions of CD31(hepatic sinusoid growth marker) were higher in experimental group than that of control group.Conclusion Exogenous protein DCN could promote the regenration of liver of fibrosis after partial hepatectomy on mice.