The Research About The Efficacy Adopting L4Transposition To Reconstruct The Function Of Sciatic Nerve

Pelvic fracture is a common fracture in clinic, which may induce the Ventral rootavulsion. or ventral injury with the functional impairment of movement and feeling of lowerlimb. Nowadays, we have no efficient method to deal with them. To further study the injuryof sacral plexus root, we need animal experiments. Rat is the ideal model in the study ofsacral plexus root. in this article,we emphasis on the animal researches of adopting thenormal sacral plexus root to reconstruct the injured sacral plexus root. We use many ways toevaluate the efficiency of this kind of therapy post-operation.Part OneTo evaluate the efficiency of adopting L4transposition to Reconstruct the sciatic nerveMethods:30adult SD rats were chosen to establish the animal model of sacral plexusroot avulsion at random after avulsing the right L4-6out of intervertebral foramina. Rats weredivided into3groups(n=10). In one group rats we did not reconstruct the sciatic nerve. Inanother groups we adopted the L4transposition to reconstruct the injured sacral plexus root.After the model establishment,12weeks after operation, rat in each group were selected forthe histomorphology of the nerves was observed under the microscope and the electronmicroscope. The models were evaluated by the observation of the survival rates of the rates,BBB scores, electron microscope weight and muscle fiber CSA(cross section area) of doublebiceps femoris, triceps surae and tibial muscle.Results:12weeks after operation, the BBB scores indicated significant differences（P=0.001） between3groups. We found remarkable significances between the ratio ofweight and muscle fiber CSA of double biceps femoris, triceps surae and tibial muscle. the Efficiency was different between the two groups (L4-L5and avulsing), the former was better.In the L4-L5group, the recovery of biceps femoris, triceps surae and tibial muscle wasdifferent. The former was better. The histomorphology of the nerves was observed under themicroscope and the electromicroscope.Conclusion: L4transposition can reconstruct the partial function of sciatic nerve the inparaplegia rats. Objective: To evaluate the degeneration of motor neurons in cornu anterius medullaespinalisMethods:30adult SD rats were chosen to establish the animal model of sacral plexusroot avulsion at random after avulsing the right L4-6out of intervertebral foramina. rats weredivided into3groups(n=10). GroupA-C.12week after operation, degeneration of motorneurons in cornu anterius medullae spinalis was elevated by HE and Tunnel.Results: The survival rates of motor neurons in cornu anterius medullae spinalis decreasedgradually and apoptosis index increased gradually. The survival rates and apoptosis index ofmotor neurons in cornu anterius medullae spinalis changed greatly in L4-L5group.Conclusion: in L4-L5group the survival rates and apoptosis index of motor neurons incornu anterius medullae spinalis changed greatly. Objective: To evaluate the efficiency of adopting L4transposition to Reconstruct thesciatic nerve in brainMethods: After12weeks, mice were subjected to PET/CT analysis. PET/CT imaging wasperformed on an Inveon MM Platform (Siemens Preclinical Solutions, Knoxville, Tennessee,USA) with a computer-controlled bed and8.5cm transaxial and5.7cm axial fields of view(FOV). The animals were anesthetized with2%isoflurane in O2gas for [18F]-FDG injection (a single injection of0.1ml FDG with an activity of10MBq intravenously in the tail vein aspreviously described), immediately awakened afterwards and placed back in the anesthesiacage. Two hours after administration of the tracer injection, animals were anesthetized withisoflurane, placed prone on the PET scanner bed near the central field of view and weremaintained under continuous anesthesia during the study with1.5%isoflurane in oxygen at2L/min. Inveon Acquisition Workplace(IAW)1.5.0.28was used for scanning process.10minCT X-ray for attenuation correction was scanned with a power of80Kv and500uA and anexposure time of1100ms before PET scan. Ten-minute static PET scans were then acquired,and images were reconstructed by an OSEM3D (Three-Dimensional Ordered SubsetsExpectation Maximum) algorithm followed by MAP (Maximization/Maximum a Posteriori)or FastMAP provided by IAW. The3D regions of interest (ROIs) were drawn over the heartguided by CT images and tracer uptake was measured using the software of Inveon ResearchWorkplace (IRW)3.0. Individual quantification of the [18F]-FDG uptake in each of them wascalculated. Mean standardized uptake values (SUV) were determined by dividing the relevantROI concentration by the ratio of the injected activity to the body weight.Conclusion: L4transposition can reconstruct the partial function of sciatic nerve the inparaplegia rats.