| Forty-six Herbages on Hulunbeir meadow steppe were ensiled separately and mixedly in this study,and composition and nutritional quality changes of fatty acids, amino acids and vitamins before andafter ensiling were evaluated, and main conclusions are as follows:1. Fatty acids of forty-six herbage silages ensiled separately and mixedly were mainly six kinds oflong-chain fatty acids, and in which, contents of linolenic acid, palmitic acid, and linoleic acid werepredominant; eighteen kinds of amino acids were detected in these silages, including eight kinds ofessential amino acids; five kinds of vitamins were also detected, in which contents of vitamin C werethe highest, while vitamin B1and B2were relatively low.2. Contents of long-chain fatty acids, amino acids and vitamins varied among four grassland types anddifferent silage treatments, and mixed ensiling had a big impact on the changes of long-chain fatty acidscontents; contents of total amino acid and essential amino acid decreased significantly after ensilingseparately (P<0.05); contents of vitamins all decreased after ensiling; There was obviously difference oflong-chain fatty acids, amino acids and vitamins contents among sample plots of different type ofgrassland.3. The ether extract and total fatty acids（TFA）contents of forty-six silages ranged from1.35%to10.85%and0.0376%to3.2505%, respectively. The value of SFA/TFA, MUFA/TFA, PUFA/TFA andUFA/TFA were7.53%-88.27%,3.33%-72.24%,7.05%-73.70%and11.73%-92.47%, respectively;There were seven silages with SFA: MUFA: PUFA reaching to or approaching1:1:1;6silages werewith n-6/n-3reaching to or approaching (4-6):1. Comprehensive evaluation suggested that compositionof fatty acids in herbages silages on Hulunbeir were unbalanced.4. The crude protein contents of forty-six herbage silages ranged from5.85%to23.67%, and contents oftotal amino acids (TAA) ranged from6.15%to20.41%. Contents of essential amino-acids (EAA),sulfur amino acid and flavor amino acids varied from2.39%to9.56%,0.12%to1.61%, and2.43%to6.77%, respectively, contents of limited amino acids were high, and the value of BCAA/AAA rangedfrom0.98%to4.29; There were89%of the silages with EAA/NEAA higher than60%, and EAA/TAAhigher than40%, and98%of the silages were higher than FAO/WHO reference protein; Comprehensiveevaluation by AAS, CS, SRC, EAAI, NI and fuzzy pattern recognition suggested that composition ofamino acids in herbage silages on Hulunbeir were balanced, and were excellent protein resources forlivestock feed.5. The content of vitamin C and β-carotene of forty-six herbage silages were relatively high, andranged from5.40mg/100g to84.87mg/100g and0.48mg/100g-12.46mg/100g, respectively.6. The correlation analysis of nutrient components among forty-six herbage silages showed that: therewas no obvious correlation between ether extract contents and fatty acid contents, while there werecomplexive correlations between different fatty acids; crude protein contents were significantly positivecorrelated with Tyrosine, Histidine, Arginine, Threonine and Valine, and extremely significant positivecorrelated with Isoleucine, Iysine and Tryptophan, and there were complexive correlations between different amino acids; crude protein contents was positively correlated with vitamin E, and there werecomplexive correlations between five vitamins.7. To evaluate forty-six herbage silages on Hulunbeir, comprehensive cluster analysis, principalcomponent analysis and fuzzy comprehensive evaluation were conducted, and the results indicated thatsilages of different herbages families on Hulunbeir had stable nutrition quality with protein and vitamin,and micro nutritive value of Leguminous forage silages were relatively high. |
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