Research On Key Technology To Immunoagglutination Quantitative Detection Performed On Microfluidic Chip With Electric Control Micro Mixer

Immunoagglutination technique is a biochemical detection technology and has been widely used in the area of pharmaceutical, agriculture, food, etc. As an advanced research task of present, immunoagglutination quantitative determination technique based on microfluidic chip is to achieve immune agglutination detection in micronano scale, and has been demonstrated to have virtues of less consumption of reagent, low detection limit, high automation degree, etc.However, there are two challenges remain in immunoagglutination technique: First, how to break free of the shackles of the laminar flow system in microscale thus realize sufficient mixing of the sensitized latex reagent and antibody(antigen) in the microchannel; Second, how to select parameters, reduce the interference and error of the detection, to achieve high-precision quantitative detection in microscale. All of these are key technologies and research focus for immunoagglutination quantitative determination technique.We summarized correlation research of the micromixer and photoelectric detection technology, and improved understanding of immunoagglutination characteristics, then introduce an microfluidic chip with electric control micro mixer for immunoagglutination quantitative detection, and give a further discussion on the key technology. The main contributions of this dissertation are as follows:Firstly, we introduce the theoretical basis of the method, and demonstrate the theoretical feasibility for microfluidic chip with electric control micro mixer for immunoagglutination quantitative detection. Secondly, the microfluids flow states that driven by the dynamic wall electromotive force are analyzed, and we have demonstrated that driving electrodes yield dynamic potential to promote flow mixing is feasible. And the physical model of promoting micro-hybrid was established, as well as corresponding governing equations and boundary conditions. The chaotic anti-control algorithm can be applied to the wall electrode were screened, and then we established the mixed chaotic anti-control evaluation system. Thirdly, the Granularity and environment parameter showed a high sensitivity for the photodetector in conditions of the microscale. We study the detection model, process parameter of the high-precision scattering detection, as well as environmental parameters, error compensation method of the absorbance detection in corresponding condition. Finally, to demonstrate the designed method, we developed a microfluidic chip with electric control micro mixer and a chaos controller. An integrated experiment platform is founded for sample introduction, mixing and photoelectric detector. The needed parameters is setted coupling the mixing process is optimized in the whole experiment with photoelectric detection process. The performance is compared with the conventional scale immune agglutination turbidimetry.The dissertation contains the following innovations:1. To electric driving mixing, the concept of mixed chaotic anti-control is presented in this thesis, namely apply the chaos electric field to the microelectrode of microfluidic chip to drive the control of fluid chaotic mixing, and the kinds of flow control methods via different chaos algorithms are compared.2. To the characteristics of the chaotic farm system and chaos fluid system, we introduce chaos scale quantitative evaluation methods respectively, and for the particularity of the flow system, we employ the particle tracking simulation method and via it to evaluate the chaotic scale. On this basis, the generalized synchronization relation of the chaotic farm system and the chaotic flow field system is validated. And based on the research of relations between the chaotic scale and mixing efficiency, our findings indicate that the large scale chaos electric field system can effectively improve the efficiency of mixing.3. The difficulty of the traditional methods can not effectively measure the size of the immune agglutination micro particles, we proposed a method that can effectively measure the size of the immune agglutination particle based on microscopic image processing technology, and the method is effective used into study the relationship between the size of the aggregation particle and the scattering model. We have also proposed for the first time, the scope of application of each scattering detector model and optimized selection of the sensitized latex particle size for immunoagglutination detection under the microscale condition. To light scattering detector particularly under microscale conditions, we first discussed the scattering model transit characteristic that generate from the aggregation particles which granularity scale is proximity detection optical wavelength. And on this basis, we screened out the best detection angle.4. We also analyzed the short optical path and constant under the microscale conditions, with the change of detection error that caused by to-be-detected changing of the concentration, and to establish the error compensation model.5. Here we achieved an electronically hybrid controlled microfluidic, and its accompanied chaotic electric field controller. First time we fabricated microfluidic test system based on electronically hybrid controlled, and created the mixed-scale validation mode under the immune agglutination condition. Several studies on electronically hybrid controlled were limited to simulation at present.In the final section, we using the optimized synthetic parameters, the quantitation detection experiment is processed on the immune agglutination quantitative detection experiment platform of microfluidic chip with electric control micro mixer, and comparing with the quantitative test results under the conventional scale. The results demonstrate that our method achieved the fully automated process from the reaction to the detection, to the detection accuracy of the rheumatoid factor is also close to the conventional scale, but the detection limit is about60%～85%lower than the conventional scale, and the liquid consumption is about one-thousandth of the conventional scale. This work provides theoretical and experimental guidance for developing the electrical control micromixer based on the microfluidic chip, for researching and developing the microscale high-precision optical testing equipment, and designing the immune agglutination detection of micro-automation machine system based on the microfluidic chip.