Effect Of Tongxieyaofang Ultramicrodrug Piece On Gastrointestinal Hormone Of D-IBS Of Clinical And Experiment Research

Part I Clinic research on D-IBS by Tongxieyaofang ultramicro-drug.Objective:To evaluate the clinical effect of Tongxieyaofang(TXYF) ultramicro-drug(UMD) on diarrhea-Irritable bowel syndrome (D-IBS), and the gastrointestinal hormone changes.Method:60 patients with D-IBS were divided randomly into two groups,treated with one dose traditional TXYF(TTXYF) and one third dose TXYFUMD(1/3TXYFUMD) respectively, two weeks for one period of treatment, the glucagon-like peptide-1 (GLP-1), Somatostatin (SS) and motilin (MTL) were selected as evaluating items.Results:1. The total effect rate of TTXYF and 1/3TXYFUMD were 96.7% and96.7% respectively and the Chinic efficiency were equal, The effectiue rate of 1/3 TXYFUMD was higher than that of TTXYF (p<0.05). 2. Two groups could reduce GLP-1, SS and MTL, and there were no significant difference between them.Conclusion:TXYF could improve the symptom of D-IBS, the efficienly between them are equal. TXYF may inhibit the secretion of GLP-1, SS and MTL. Part II The effect of TXYF UMD on D-IBS rats GLP-1, SS and MTL.Objective:To explor the effect of TTXYF and TXYF UMD on GLP-1, SS and MTL content in tissue and serum of normal rats and D-IBS rats.Method:60 SD rats were randomly divided into normal contrast group, D-IBS group, traditional TXYF group, divided into normal contrast group, D-IBS group, traditional TXYF group, TXYF UMD group,1/3 TXYF UMD group,1/6 TXYF UMD group. D-IBS groups were induced by drugs and bunch, the mormal contrast group were given high pressure disinfection water. To have a experiment of deliver of bowel and costringency experiment of abdominal muscles after 3 days. To collect the excrement of rats and measure its dry weight and wet weight. Withdraw specimen after 2 weeks.measure the level of GLP-1, SS and MTL of tissue and serum with the method of radioimmunoassay way (RIA).Results:1. the tissue and serum GLP-1, SS,SP, MTL in the TXYF UMD group were lower than those in TTXYF group (p<0.01).2. The tissue and serum GLP-1, SS,SP and MTL in the TXYF UMD group,TTXYF group, and 1/3 TXYF UMD group were all lower than those in D-IBS group (p<0.01). but there were no significant difference between 1/6 TXYFUMD and D-IBS group. 3. The tissue and serum GLP-1,SS,SP and MTL in the D-IBS group were higher than those in other group (p< 0.01), and there were no significant difference between the 1/6 TXYFUMD group and D-IBS group (p>0.05).4.The tissue and serum GLP-1,SS,SP and MTL in the TXYFUMD group were all lower than those in TTXYF group (p< 0.01), and there were no significant difference between the 1/3 TXYFUMD group and TTXYF group (p>0.05)Conclusion:1.TXYF has the function to regulate the gastrointestinal hormone for rats.2.1t may reduce the secretion of GLP-1, SS,SP and MTL.3. The TXYFUMD may reduce the secretion of GLP-1mRNA and SSR1RNA of D-IBS rats.4. The function of TXYFUMD may be more superior to function of TTXYF.