Data Mining Of Tong Yang Medicine And Experimental Research Of Regulating Dyslipidemia On Extract Of Bulbus Allii Caespitosi

The thesis is divided into two parts, the first part of the analysis is based on data mining to explore yang of Chinese medicine Characteristics and classification, and the second part is the experimental study of dyslipidemia Onion extract regulation。The first part of a retrospective analysis of the literature data and use data mining techniques to explore the characteristics of traditional Chinese medicine and yang yang summarized classification methods. We "Chinese Medical Classics" ("Chinese Medical Classics, " a collection of ancient Chinese medicine classics Republic before 1000, voluminous ten thousand, nearly 400 million words, a collection of previous writings of ancient Chinese medicine major), the use of computers to retrieve, to "pass Yang "as the search term, the search to the relevant provisions of 892. After reading the provisions discussed included the role of a traditional Chinese medicine has a yang provisions for the study. Reference to the relevant textbooks and publications on literature data preprocessing, including error correction and normalization filled data. Studies with frequency, cluster analysis to explore the role of traditional Chinese medicine yang characteristics and classification. This study were collected through 38 male Chinese flavor, the provisions concerning 292 times. From medicinal taste point of view, the probability of finding yang of Chinese medicine to pungent, warm up to, pungent and hot sex together is the highest, is the most common,38 yang flavor taste of traditional Chinese medicine, there are 19 Xin Wen (heat) yang of Chinese medicine, Xin Wen (heat) through yang yang method is the most common method, can be divided into medicine Xin Tong Yang Wen (hot) yang of Chinese medicine, bitter cold yang of Chinese medicine, Chinese medicine sun light to pass. From the perspective of Meridian found yang herbs on the heart, liver, spleen, lung, kidney effect is more obvious, traditional Chinese medicine can be divided into Xuan Tong Yang Tong Lung Yang, Wen Tong Xin Yang, smooth Spleen, Liver Yang dredge, around the kidney and so on. Onion Xin Wen, lung, stomach, and acrid, warm air is four flavors appear most frequently, lung, stomach meridian is also appearing in most of the typical characteristics of traditional Chinese medicine with yang. Yang of Chinese medicine and involves 292 times, and has 110 times the frequency of occurrence for most, this we can infer Onion yang of Chinese medicine as a representative of the drug.The second part is based on our previous studies, in order to pass yang of Chinese medicine for the study on behalf of the drug Onion, Onion extract investigate the mechanism regulating dyslipidemia.32 male SPF SD rats (180±20) g, were randomly divided into control group, model group, Xuezhikang group, Onion extract group 4 groups of 8。In this study, by feeding high fat diet (high fat diet recipe: 82.3% of the basal diet added lard 10%,2% olesterol,5% sucrose, 0.5% sodium deoxycholate,0.2% sulfide from Beijing, China Fukang alloxan bio-Technology Co., Ltd. production provided) 6 weeks in hyperlipidemic model, feeding six weeks after CHOL and LDL-C increased significantly (P<0.05), modeling success. Onion extract provided by the Wuhan City Central Hospital Integrative preparations. According to the translation of human animal doses were given 42mg/kg Onion Onion extract extract orally, Xuezhikang group received 250mg/kg Xuezhikang gavage, the control group, model group were given the same volume of saline once a day. Each group were administered continuously over six weeks later, Onion Extract, Xuezhikang can be significantly reduced CHOL, TG, LDL-C levels, increased HDL-C levels (P<0.05). Compared with the control group Xuezhikang, Onion extract superior Xuezhikang (P<0.05) in reducing CHOL, TG, LDL-C levels, model after model of liver index increased significantly (P<0.05), while the weight instead, lighten, indicating lipid deposition in the liver, weight loss may be associated with the decline in rats fed high-fat diet-related appetite. And all drugs on body weight and liver index level has little effect, Onion extract can also reduce lipid deposition in the liver, kidney and epididymal adipose tissue, its role and Xuezhikang no significant difference, with good prospects for clinical use.We used gene chip technology research Onion extract on blood lipid regulation of genes, gene expression, model and Onion extract DNA microarray data set of standardized according to the difference in multiples (fold change) screening differences, screened more than 2-fold upregulated and there were significant differences in gene 59, including 16 upregulated genes downregulated genes 43. Literature features a comprehensive analysis of these genes and found that closely related to the regulation of lipid genes mainly carnitine palmitoyl transferase 1B (carnitine almitoyltransferase lb, muscle, Cptlb), induces cell death DNA fragmentation factor-like effect of a factor A (cell death-inducing DFFA-like effector a, Cidea), apolipoprotein 2 (lipocalin 2, Lcn2), stearoyl-coenzyme A desaturase (stearoyl-coenzyme Adesaturase, SCD), fibroblast growth factor -21 (fibroblast growth factor 21, FGF21) and the likeAnd the use of real-time PCR technology to detect the expression of liver tissue FGF21 SCAP LXRs (LXR α, LXR β) of SREBP-lc Western-blot detection of liver tissue SREBP-1 expression, SREBP-2’s., Onion extract was found lipid regulating mechanism may increase FGF21, gene expression, Cptlb, reduce Lcn2, SCD gene expression and SREBP-1, protein levels of SREBP-1C, but not with SCAP gene and LXR-α, LXR-β, little to SREBP-2 protein, Onion extract cholesterol-lowering mechanism needs to be further explored. Previous studies found that SREBP-lc induced expression of SCD, SCD activate CPT1 activity, the three have some connection, Onion extract lipid regulating mechanism may be associated with reduced protein expression of SREBP-lc and SCD, while expressing CPTlb upregulation oxidation of fatty acid β-related enhancements. Previous studies found that SREBP-lc is FGF21 inhibition of nuclear sterol regulatory element binding transcription factor, our study showed that rats Onion extract protein SREBP-lc and FGF21 gene down, Onion extract lipid regulating mechanism Onion extract lipid regulating mechanism may be associated with reduced SREBP-lc protein, increased expression of FGF21 increase fat utilization, consumption and transport are closely related Meanwhile Onion extract may also reduce liver TC, TG content, which may also lowered SCD, upregulate the expression of FGF21-related. Onion extract can decrease the expression of Lcn2. Onion extract lipid regulating mechanism may be associated with reduced preadipocytes into fat cells。In addition, microarray results, FGF21 is Onion extract differences in genes regulating blood lipids, and SCAP is not consistent with the results of quantitative PCR gene chip microarray results more reliable。Xin Wen Tong Yang on behalf of drug Onion extract has good blood lipid regulation and its mechanism of action is to reduce TG TG while reducing the generation, on the one hand to promote energy metabolism, enhance oxidation of fatty acid β-related; while cholesterol-lowering mechanism needs to be further explored.