| Objective AS arterial wall tissue of rats by observing the light blue of different forms of active ingredient, antioxidant, anti-inflammatory, differences in regulation of lipid research, a clear light blue effect of different active ingredients, explore Xin Wen Tong Yang Chinese Onion atherosclerosis in rats therapeutic effect and mechanism of atherosclerosis, the initial reveal Xin Wen Tong Yang prevention material basis of AS.Methods Select the weight was 200 ± 20 g male SD rats 70, sorted according to body weight were randomly divided into normal group, model group, Bulbus Allii Caespitosi extract group, light blue volatile oil group, the group of flavonoids light blue, light blue steroidal saponins, light blue fatty acid groups, each group 10. Preparation of atherosclerosis models. Adaptive feeding after a week, the normal group underwent sham surgery, the rest of the group underwent balloon injury of rat aorta surgery. Normal group was fed the basal diet and ordinary tap water, the model group and the treatment group was given high fat diet( 4% cholesterol, 0.5% sodium cholate, 10% lard, 0.2% propylthiouracil and 94.3 % of the diet), and at the beginning of theexperiment on the right lower limb intramuscular injection of vitamin D3(3 × 105 u / kg body weight), after repeated injections once every 30 days. The first three days after surgery began dosing, dosing volume of 2ml / kg, group and model group were given normal saline. Bulbus Allii Caespitosi extract group administered by 60mg/100 g, light blue volatile oil group 6mg/100g(10%), light blue flavonoid group 0.6mg/100g(1%), light blue steroidal saponins group 15mg/100g(25%), light blue fatty acid group 9mg/100g(15%),; 1 times a day for consecutive 16 weeks. After 16 weeks of fasting experiment 16 h, anesthetized animals, abdominal aortic blood serum was separated and stored at-80 ℃ low temperature refrigerator spare. 16 weeks after the experiment were fasted 16 h, animals were anesthetized, the initial segment of the aortic arch aortic specimens taken 1cm, 10% were fixed with paraformaldehyde 12 hours; open the abdominal cavity, separated extravascular adipose tissue, cut along the midline of the back of the aorta, saline flush,-80 ℃ low temperature refrigerator for use. HE staining by oil red O staining of pathological changes and pathological ingredients. Serum total cholesterol in serum(total cholestero, TC), triglycerides(triglyceride, TG), high density lipoprotein cholesterol(high-density lipoprotein, HDL-C), low density lipoprotein cholesterol(low-density cholesterol lipopmtein, LDL-C), total antioxidant capacity(total antioxidant capacity, T-AOC), malondialdehyde(malondialolehyde, MDA), superoxide dismutase enzymes(Superoxide Dismutase, SOD) levels. Serum IL-6 levels by ELISA. Using real-time PCR technology to detect PPARγm RNA, CXCL-16 expression in the abdominal aorta.Results 1. HE staining showed pathological changes of the common carotid artery Normal lumen rounded, media and intima and adventitia boundaries clear, lumen covered by a single layer of endothelial cells, structural integrity, continuous internal elastic plate without fracture, medial and intimal hyperplasia no, no inflammatory cell infiltration; model group : the membrane and outer membrane unclear boundaries, the film marked hyperplasia, endometrial thickening, not smooth and continuous shedding of endothelial cell injury absence of visible plaque highlighting the lumen, plaque contains a large number of giant macrophages, relatively few smooth muscle cells and collagen content; Bulbus Allii Caespitosi extract group relatively mild degree of intimal thickening, endothelial cells arranged in neat, continuous elastic membrane no fracture, no plaque rupture; Onion steroidal saponins film group, light blue volatile oil group relatively mild hyperplasia, endothelial cells arranged in a relatively neat, visible elastic membrane rupture, see also plaque rupture; light blue flavonoid group, Onion fatty acid group collagen extract group see clearly, the film thickness, the structure is not clear, loss of endothelial cell injury. 2 Oil Red O staining observation of abdominal aortic lipid deposition Normal group, no significant lipid droplets, a significant increase in the fat content of the untreated group; Bulbus Allii Caespitosi extract group of fat, light blue, light blue steroidal saponins group of fatty acids group was significantly lower fat content, light blue flavonoid group, a significant increase in the fat content of volatile oil group Onion. 3 TG and TC ’s impact on AS rat Compared with the normal group, model group rats TC, TG was significantly higher(P <0.01); compared with the model group, Bulbus Allii Caespitosi extract group TG lower(P <0.05), significantly lower TC significantly(P <0.01); with the model group Compare Onion steroidal saponins group TG lower(P <0.05). Significantly reduce the relatively volatile oil Onion TC group and model group(P <0.01). Compared with the model group, light blue fatty acid group, light blue flavonoid group TC, TG were not statistically different(P> 0.05). Compared with Bulbus Allii Caespitosi extract, TG was no significant difference(P> 0.05) Onion steroidal saponins group. Compared with Bulbus Allii Caespitosi extract, TC was no significant difference(P> 0.05) Onion volatile oil group. 4 on HDL-C and LDL-C in Compared with the normal group, HDL-C levels in rat model group decreased(P <0.05), LDL-C levels were significantly increased(P <0.01); compared with the model group, Bulbus Allii Caespitosi extract group increased HDL-C levels(P <0.05), LDL-C levels were significantly lower(P <0.01); compared with model group Onion steroidal saponins increased HDL-C group(P <0.05). Compared with the model group Onion fatty LDL-C decreased significantly(P <0.01), compared with the model group, light blue volatile oil group, light blue flavonoid group HDL-C, LDL-C levels was statistically no difference(P> 0.05). Compared with Bulbus Allii Caespitosi extract, no significant difference(P> 0.05) Onion steroidal saponins group HDL-C levels. Compared with Bulbus Allii Caespitosi extract, no significant difference(P> 0.05) Onion fatty acid group LDL-C levels. 5 on rat aorta PPARγm RNA expression of AS Compared with the normal group, model group was significantly lower abdominal aorta PPARγm RNA; compared with the model group, Onion Bulbus Allii Caespitosi extract group and the group of steroidalsaponins PPARγm RNA increased expression of the abdominal aorta. Multiple gene amplification in each group were: Bulbus Allii Caespitosi extract group : 1.81; Onion steroidal saponins 1.87; Onion flavonoid group 0.59; Onion fatty acid group : 0.50; Onion volatile oil group : 0.55; model group : 0.57 6 AS impact of serum T-AOC Compared with the model group, Bulbus Allii Caespitosi extract group, light blue and light blue steroidal saponins group of flavonoids group were significantly different(P <0.01). Compared with Bulbus Allii Caespitosi extract, light blue and light blue steroidal saponins group of flavonoids group were significantly different(P <0.01). Steroidal saponins Onion Onion flavonoid group was better than group(P <0.05). 7. Effect on serum SOD AS rats Compared with the model group, Bulbus Allii Caespitosi extract group, light blue and light blue steroidal saponins group of flavonoids group were significantly different(P <0.01). Compared with Bulbus Allii Caespitosi extract, light blue and light blue steroidal saponins group of flavonoids group were significantly different(P <0.01). Onion steroidal saponins group was superior light blue flavonoid group(P <0.01). 8 on serum MDA AS rats Compared with the untreated group, Bulbus Allii Caespitosi extract group, light blue and light blue steroidal saponins group of flavonoids group were significantly different(P <0.01). Compared with Bulbus Allii Caespitosi extract, light blue and light blue steroidal saponins group of flavonoids group were significantly different(P <0.01). No significant difference(P> 0.05) Onion steroidal saponins group action with light blue flavonoid group. 9 For AS the serum IL-6 in the ratCompared with the normal group, serum IL-6 levels in rats in the model group increased(P <0.05), compared with the model group, light blue and light blue extract group steroidal saponins group were significantly different(P <0.01). Compared with Bulbus Allii Caespitosi extract, Onion steroidal saponins group were significantly different(P <0.01). 10. Effect on CXCL-16 expression in rat aorta AS Compared with the normal group, model group was significantly increased abdominal aortic CXCL-16; Onion Bulbus Allii Caespitosi extract group and the group of steroidal saponins abdominal aorta CXCL-16 expression compared with the model group decreased. Changes in gene amplification multiples of each group are as follows: Bulbus Allii Caespitosi extract group : 0.78; Onion steroidal saponins 0.49; Onion flavonoid group 1.06; Onion fatty acid group : 1.26; Onion volatile oil group : 1.19; model group : 1.49. 11.Effect of serum NO in rats of the AS Compared with the model group, the group of onion onion extract, volatile oil, steroidal saponins group group of onion and onion flavonoids group were significantly different(P<0.01). Compared with the onion extract, fistular onion stalk steroidal saponin group and ose flavone group were significantly different(P<0.01). Compared with the onion extract, no statistical difference of fistular onion stalk volatile oil group(P > 0.05). 12.Effects of plasma H2 S on AS rats Compared with the model group, the group of onion onion onion extract, volatile oil group were significantly different(P<0.01). Compared with the onion extract, fistular onion stalk steroidal saponin group and ose flavone group were significantly different(P<0.01). Compared with the onion extract, no statistical difference of fistular onion stalk volatile oilgroup(P > 0.05).Conclusion 1 AS Bulbus Allii Caespitosi extract reduces intimal injury induced pathology is best to reduce the vascular wall lipid deposition. 2 Bulbus Allii Caespitosi extract group had elevated HDL-C, reduce the role of TC, TG and LDL-C ’s; Onion steroidal saponins with elevated HDL-C, reduce the role of TG. Onion volatile oil has reduced the role of TC. Onion fatty acids have a role in reducing LDL-C. Onion Bulbus Allii Caespitosi extracts and steroidal saponins may increase the expression of PPARγm RNA on the abdominal aorta. 3 Bulbus Allii Caespitosi extract can reduce serum MDA, SOD and T-AOC elevated levels, light blue and light blue steroidal saponins flavonoids may reduce serum MDA, SOD and T-AOC elevated levels but the effect was weaker than both Bulbus Allii Caespitosi extract. 4 Onion Bulbus Allii Caespitosi extract and steroidal saponins can significantly reduce serum IL-6 levels, light blue and light blue extract steroidal saponins reduce CXCL16 expression in the abdominal aorta. 5 onion extract, volatile oil group, group of onion onion steroidal saponin group and ose flavone group have significant difference could significantly increase the level of serum NO, fistular onion stalk extract group, volatile oil of fistular onion stalk group can significantly increase the plasma level of H2 S. 6 yang effect Onion extract volatile oil is Bulbus Allii, Bulbus Allii flavonoids result, Bulbus Allii and Bulbus Allii fatty steroidal saponins synergy. |
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