Multi-ingredient Traditional Chinese Medicines Preparation Impact On Levels Of Mouse Model For ITP Cytokines And Bcl-2, TPO

Objective：Immune thrombocytopenia (immune thrombocytopenia, ITP) is a result ofan autoimmune bleeding disorder caused by abnormal, with a lingering disease, recurrentrefractory characteristics, we found that in clinical studies, chronic ITP patients in a "positive imaginary evil is real,"the pathogenesis, clinical deficiency is not taken to yindeficiency type and the most common type. In this study, we were measured traditionalChinese medicine bu qi Xiao dian Tang and yang yin xiao dian tang on ITP model withABC-ELISA method，to check the content of ITP mice serum IL-2, IL-6, IL-10, TGF-β1,TNF-α and apoptosis gene Bcl-2and serum thrombopoietin (TPO), to explore traditionalChinese medicine treatment ITP mechanism of action and provide the basis for clinicalmedicine.Methods：1.By intraperitoneal injection of guinea pig anti-mouse platelet serum (APS)immune modeling method to establish BALB/C mice model of ITP, By detecting plateletcount, spleen index calculation, counting the total number of bone marrowmegakaryocytes and capacity plate number of megakaryocytes, for model evaluation.2.The72BALB/C mice were randomly divided into six groups, namely normal group,model group, prednisone group, Wei Xue Ning group, Chinese medicine1(bu qi xiaodian tang)group, traditional Chinese medicine2(yang yin xiao dian tang) group.Aftertreatment, mice ITP general condition improved pathological spleen, spleen index, bloodplatelet count, the total number of bone marrow megakaryocytes, middle board changes inthe number of megakaryocytes.3. By double antibody sandwich ABC-ELISA method todetecte six serum levels of cytokines IL-2, IL-6, IL-10, TGF-β1, TNF-α, apoptosis geneBcl-2and serum TPO concentration changes.Result：1.After using anti-mouse platelet serum (APS) were established animal model,Model mice significantly reduced platelet and spleen significantly increased the total number of bone marrow megakaryocytes increased significantly, significantly reducedmegakaryocytes middle board, which meets the clinical features of human ITP, The ITPanimal mode can be used as an experimental study animal immune model.2. Comparedwith the model group,bu qi xiao dian tang and yang yin xiao dian tang could significantlyimprove the general condition of ITP mice, Increase in body weight of mice (P <0.01),reduced swelling of the spleen, increase blood platelet count, and bone marrowmegakaryocytes total production board, reducing the total number of megakaryocytes inthe bone marrow, significant difference (P <0.05, P <0.01).3. By ABC-ELISA assay,confirmed bu qi xiao dian tang and yang yin xiao dian tang can significantly reduce serumITP model cytokines IL-2, IL-6, TGF-β1, TNF-α cytokine levels (P <0.01, P <0.05),increased mice concentrations of IL-10(P <0.01), decreased apoptosis genes bcl-2levels(P <0.05), lower blood thrombopoietin (TPO) levels (P <0.01).Conclusion: The results of this study show that bu qi Xiao dian tang and yang yinXiao dian tang has significantly increased the role of platelets. Its work through thefollowing points:⑴vivo inhibition of Th1cytokines IL-2, IL-6, TNF-α activity,increased Th2cytokines IL-10concentration, adjust the body Th1, Th2cell functionimbalance, inhibition of T cell-mediated immune response, and by inhibiting theproliferation of B lymphocytes, activation, reduce platelet antibodies.⑵reduce bodyapoptosis gene bcl-2protein content, promote apoptosis of activated lymphocytes,immune imbalance.⑶reduce body TGF-β1levels, reduce production board marrowmegakaryocytes inhibition and promote their further differentiation, maturation;⑷bystimulating megakaryocyte progenitor cell proliferation, differentiation, maturation, andpromote megakaryocyte TPO and TPO receptors on cells, thereby reducing peripheralTPO concentration. These studies show that bu qi Xiaodian tang and yang Yin xiao dianTang on ITP mice with good l-platelet effect for clinical application provides a reliablebasis.