Objective Research Of Differences Between Splenic Asthenia And Phlegm-damp Syndrome And Depression Of Lung And Stagnation Of Phlegm Syndrome Of Non-small Cell Lung Cancer

Objective1 Clinical studyThe study was to find the differences of immune index (T lymphocyte, NK cell and B lymphocyte) and tumor markers (CEA, CA125, CA15-3 and CA199) between the splenic asthenia and phlegm-damp syndrome and the depression of lung and stagnation of phlegm syndrome of non-small cell lung cancer, and try to provide evidence for the TCM diagnosis and treatment of non-small cell lung cancer.2 Experimental studyThe study analysed serum proteomics of splenic asthenia and phlegm-damp syndrome and depression of lung and stagnation of phlegm syndrome of non-small cell lung cancer, screened the differentially expressed proteins, and found the proteins which were related with the two TCM syndromes. Then we explored the protein expression and the relationship between proteins and TCM syndromes, so as to provide objective basis for syndrome differentiation of non-small cell lung cancer.Methods1 Clinical studyWe chose 30 cases of non-small cell lung cancer patients with splenic asthenia and phlegm-damp syndrome and 30 with depression of lung and stagnation of phlegm syndrome, collected their general data, clinical physicochemical index, immune index and tumor markers, and then we used the statistical analysis software of Stata to analyse the differences of the expression of the immune index and tumor markers between two symptoms.2 Experimental studyWe analyzed the serum proteins of 3 patients with splenic asthenia and phlegm-damp syndrome of non-small cell lung cancer and 3 with depression of lung and stagnation of phlegm syndrome by appling two-dimensional fluorescence difference gel electrophoresis (2D-DIGE), then scanned the gel pictures and found out the differentially expressed proteins with DeCyder software. The differentially expressed proteins were identified by matrix-assisted laser desorption ionization time- of- flight mass spectrometry (MALDI-TOF-MS), and the finger print maps of peptide were obtained. Then we searched them in Protein Data Bank. At last, we searched the functions, the biological processes and the classifications of these differentially expressed proteins on the PANTHER website.Results1 Clinical study(1) We compared the NK cell (CD3/16+56) amount between splenic asthenia and phlegm-damp syndrome and depression of lung and stagnation of phlegm syndrome of non-small cell lung cancer, and found that the NK cell amount was a little more in depression of lung and stagnation of phlegm syndrome of non-small cell lung cancer than in the other, but the result is of no statistical significance (P> 0.05)(2) We compared the B lymphocyte amount between splenic asthenia and phlegm-damp syndrome and depression of lung and stagnation of phlegm syndrome of non-small cell lung cancer, and found that the B lymphocyte amount was more in depression of lung and stagnation of phlegm syndrome of non-small cell lung cancer than in the other, but the result is of no statistical significance CEA, CA15-3 and CA199 are higher in the splenic asthenia and phlegm-damp syndrome of non-small cell lung cancer than in the depression of lung and stagnation of phlegm syndrome. The results are of no statistical significance (P> 0.05).The positive rate of CA125 is higher in the depression of lung and stagnation of phlegm syndrome of non-small cell lung cancer than in the splenic asthenia and phlegm-damp syndrome, and the result has statistical significance (P<0.05).(3) We compared the T lymphocyte amout between splenic asthenia and phlegm-small cell lung cancer,and found there is no significant difference between the two syndroms.(4) We compared the level of som tunor markers between the splenic asthenia and phlegm-damp syndrome and the depression of lung and stagnation of phlegm syndrome of non-small cell lung cancer,and fpung that the positive rates of2 Experimental studyThere is six differentially expressed proteins between the splenic asthenia and phlegm-damp syndrome and the depression of lung and stagnation of phlegm syndrome of non-small cell lung cancer. Those which are up-regulated in the splenic asthenia and phlegm-damp syndrome and down-regulated in the depression of lung and stagnation of phlegm syndrome are C09 and C04A; Those which are up-regulated in the the depression of lung and stagnation of phlegm syndrome and down-regulated in the splenic asthenia and phlegm-damp syndrome are ITIH4, RLA2, AlAT and LHX5. The bioinformatics search shows that C09, C04A, ITIH4 and AlAT take part in complement activation, proteolysis and regulation of catalytic activity directly or indirectly. They belongs to serine protease or its inhibitor. The results suggests that the processes of complement activation, proteolysis and regulation of catalytic activity are different between the splenic asthenia and phlegm-damp syndrome and the depression of lung and stagnation of phlegm syndrome of non-small cell lung cancer.Conelusion1 Clinical study(1) The amounts of NK cell and B lymphocyte are more in the depression of lung and stagnation of phlegm syndrome of non-small cell lung cancer than in the splenic asthenia and phlegm-damp syndrome, but the results were of no statistical significance (P> 0.05).(2) There is no significant difference of the amounts of T lymphocyte between the splenic asthenia and phlegm-damp syndrome and the depression of lung and stagnation of phlegm syndrome of non-small cell lung cancer.(3) There is a trend that the positive rates of CEA, CA15-3 and CA199 are higher in the splenic asthenia and phlegm-damp syndrome of non-small cell lung cancer than in the depression of lung and stagnation of phlegm syndrome. The results were of no statistical significance. The positive rate of CA125 is higher in the depression of lung and stagnation of phlegm syndrome of non-small cell lung cancer than in the splenic asthenia and phlegm-damp syndrome, and the result has statistical significance.2 Experimental studyThere are differentially expressed proteins between the splenic asthenia and phlegm-damp syndrome and the depression of lung and stagnation of phlegm syndrome of non-small cell lung cancer, which suggest that there are differentially expressed proteins between different syndromes of the same disease, and the proteomics techniques can help us to discover the mechanism of some kind of TCM syndrome.