Effects Of Different Gastrointestinal Reconstructive Surgeries On The Growth And Function Of Pancreas β Cell

Part Ⅰ:The establishment of different gastrointestinal reconstructive surgeries GK rat modelsObjective:To establish the stable gastrointestinal reconstructive surgeries GK rat modelsMethods:12weeks GK rats were randomly divided into four groups (RYGB group, the SG group, the DJB group and the sham operation group). Then the rats were performed different operations by group. They were observed of eight weeks after operation. After eight weeks, the weight and the fat content were be compared by group.In the meanwhile, we assessed the surgical mortality and complication.Results:To be successful to establish the stable gastrointestinal reconstructive surgical GK rat modelsConclusion:After surgery, the weight and the fat content of GK rats in the surgical groups decreased significantly. The mortality and complications of surgery were quite few. The rat models were probed to be a stable animal model to study the mechanism of different operation. Part Ⅱ:The Effects of Different Gastrointestinal Reconstructive Surgery as a Treatment for Type-2Diabetes in GK RatsObjective:To study the effects of different gastrointestinal reconstructive surgery as a treatment for Type-2diabetes in GK ratsMethods:12weeks GK rats were randomly divided into four groups (RYGB group, the SG group, the DJB and the sham operation control group). Then the rats were performed different operations by group. They were observed of eight weeks after operation. During the obervation, the weight of the model rats were be compared by group. At2,4,6and8weeks after operation, the sample blood were be collected to assay the blood glucose, insulin, GLP-1. Furthermore, OGTT and and clamp experiments were be profermed.Results:The diabetes of GK rats was relieved after operation. The fasting blood glycose and the Glucose tolerance of the GK rats were improved a lot. The levels of the plasm insulin were elevated greatly. The results of the clamp experiments were proved that the insulin resistance of the GK rats had been relieved.Conclusion:Three different operations all provided remission of diabetes. Part III:The effects of different gastrointestinal reconstructive surgery on the growth and function of βcell in GK ratObjective:To study the effects of different surgery on the growth and function of β-cell, in order to further investigate mechanism of the operativeMethods:The GK rats after surgery in second part of the experiment were executed in8weeks. In addition,5Wistar rats were selected as normal controls. All the pancreatic specimens were weighed and fixed. Then they were made to slices for IHC staining to assess the number of pancreas islet, the number and the proportion of β-cell, proliferation and apoptosis of the β-cell, the expression of PDX-1receptors and the GLP-1receptors on the β-cell surface.Results:The pancreases of GK rats in sham operation group obvious atrophy. In the pancreas, the number of pancreas islet, the number and the proportion of β-cell, reduce greatly. The pancreases of GK rat in RYGB group were similar to the pancreas of normal Wistar rats. The morphology of the pancreas of GK rats in DJB group were in the middle of the morphology of the pancreas of GK rats in RYGB group and in sham operation group. The pancreases of GK rat in SG group were similar to the pancreas of GK rats in sham operation group.Conclusion:The remission of diabetes of the GK rats in RYGB group had relation of the almost normal pancreas. Duodenum jejunum bypass had effects of increasing the number and the proportion of β-cell. Sleeve gastrectomy had fewer effects on the pancreas of GK rats. The treatment effects of this operation relayed on the decreased weight.