Initial Research On Clinical And Mechanism Of Shuganjianpi Gusui Recipe Treat Multiple Sclerosis

Objective Studing the correlativity of peripheral blood T cells subsets in patients with multiple sclerosis of and the stage of disease.Investigate the possibilities of T-cell subs ets as indicators of multiple sclerosis changes in condition and foudation of treatment. Explore the mechanism of Shuganjianpi Gusui Recipe treat MS by EAE modal.MethodsMS patients wound be classified according to the stage of disease, statistics variation of peripheral blood T cell subsets in differate stage. Using of cluster analysis to explore the characteristics of the syndrome of multiple sclerosis. Shuganjianpi Gusui Recipe treats animal model of multiple sclerosis, detected its peripheral inflammatory cytokine protein expression and observed the pathological changes.Results1. Clinical Research:1.1CD4+T cells subsets of MS in hormone treatment stage is higher than early onset desease, period of influence hormones, stable period, difference had statistical significance (P<0.05). There was significant difference while CD3+CD19-T subsets after hormone treatment is significantly lower than hormone treatment stage (.P<0.05). The rest of the T cell subsets in each stage of disease showed no significant difference(P>0.05).1.2In clinical studies, most MS patients with varying degrees of liver and spleen deficiency syndrome characteristics. For example, depression (50.8%), two flank discomfort (22.2%), tightness in the chest(31.7%), irritability (41.3%), lassitude (65.1%), decreased appetite(28.6%), nausea (12.7%), hiccups (12.7%), belching (14.3%), abdominal distension (22.2%), epigastric fullness (28.6%).Cluster analysis of symptoms is divided into three categories:the first category:the first cluster of symptoms manifested as shuiyin, the second cluster of phlegm, and the third cluster as spleen deficiency. The second category:the first cluster of the heart and kidney yin deficiency, the second cluster of yin difiency huowang. The third category is the yang deficiency. The fourth category is the yin and yang deficiency. 2. Experimental Research:2.1MOG35-55immunization of C57BL mice obtained EAE chronic persistent model. Found that the mice first time of onset14-17days after immunization, clinical score for about1. The peak incidence on19-29days, it’s clinical score between1and2.5. Subsequently moved to a chronic stage which having clinical score remained1to2. Few mice were found having relapse phenomenon, the frequency of recurrence and symptom scores decreased with time, in about three months the relapse-remitte mouse reached the same plateau with chronic mouse.2.2Mechanisms of Shuganjianpi Gusui Recipe animal model for multiple sclerosis:2.2.1In EAE model, IL-21, IL-17F as inflammatory cytokines, increased firstly and then decreased, indicating that they play a role early in the disease. In hormone group they elevated were not obvious, in the TCM group IL-17F was a downward trend.2.2.2In EAE, TNF-a as an inflammatory cytokine gradually increased, But in the hormone group and TCM group was first decreased and then increased, especially at4week was more evident. IFN-y as pro-inflammatory cytokines in EAE was gradually increased, but in the hormone group and TCM group decreased at first and then slightly rising, and the most significant decline at4weeks.Indicating TCM group and hormones group may play a role in inhibiting inflammatory at early onset of the disease and anti-inflammatory effect gradually diminishes at late stage.2.2.3IL-10and IL-4as the anti-inflammatory cytokines in EAE gradually increased, so do the same in TCM group.The increase in the TCM group more obvious, especially at8weeks, but the hormone group does not show this trend. Point to EAE showed myelin damage in the acute phase of inflammation, but also launched anti-inflammatory mechanism as progression of desease.Hormones inhibited inflammatory cytokine production, also inhibited the anti-inflammatory cytokine production. TCM protected the endogenous positive factors of anti-inflammatory cytokine, did favor for the repair and reduce recurrence late.2.2.4Animal Pathology revealed, EAE brain tissue and spinal cord tissue slices showed obvious inflammatory cell infiltration, vascular proliferation. Normal brain tissue slices showed little inflammatory cell proliferation, few vascular angiogenesis. Conclusion1. Total T cells and CD4+T cells is higher on exacerbation of Multiple sclerosis than other stage of MS, as this rule, T cells may be to be an indicator of condition changes in multiple sclerosis.2. The mechanism of Shuganjianpi Gusui Recipe treat multiple sclerosis may be reduces the expression of pro-inflammatory cytokines, and protection anti-inflammatory cytokines to control inflammation. Although inhibition of pro-inflammatory cytokines is less than hormones, but for anti-inflammatory cytokines have a protective effect as its features of TCM. This seems to be explain that Shuganjianpi Gusui Recipe control of acute symptoms weeker than hormones, which have a reduced role in multiple sclerosis clinical recurrence.3. EAE mouse model can be used as screening target of anti-inflammatory drugs model of multiple sclerosis, TNF-a, IFN-y, IL-21, IL-17F and IL-10, IL-4are more sensible, can be used to screening drugs and determine the drugs’efficacy, provides an experimental basis for further study in MS drug therapy and drug screening.4. MOG35-55immunization of C57BL mice obtained EAE chronic persistent model. Few mice were found having relapse phenomenon, point to there may be a big difference in murine autoimmune conditions. In the application of this classic model of EAE is still aware that the possibility of causing interference for drug screening of acute exacerbation.