| This paper consists of literature review, experimental study and clinical research.Literature review summarizes the intervention of traditional Chinese medicine research progress of abnormal glucose tolerance. Firstly summarizes the physicians embalmed in IGT (PiDan) the understanding of the etiology and pathogenesis, and then analysis of the current epidemiological studies some main understanding of IGT card type, then discusses the main method and progress of TCM intervention IGT in recent years, the last of IGT mechanism of traditional Chinese medicine research content.Clinical section called TangNaiKang granules treatment in phase III clinical trials, impaired glucose tolerance, objective:further evaluation TangNaiKang granules treatment clinical curative effect and safety of IGT. Methods:multicenter, randomized, double-blind, placebo parallel control design, a total of480subjects according to the proportion of3:1randomly assigned into the treatment group (TangNaiKang granule group) and control group (placebo), a12-week intervention. Results:12wk, a drug treatment group, postprandial2hours blood glucose changes than the control group compared with statistical significance (P<0.05), treatment group was superior to control group. For12wk, a FBS, FINS,2hins compared with baseline, the two groups have no statistical difference, but there is a decrease in the treatment group than the control group in HbAlc data trend. Blood lipids observed indexes were no significant difference between two groups. Significant difference was found in TCM syndrome integral. Treatment group total effectiveness87.3%, after blood sugar often rate53%, TCM syndrome total effective rate95.8%, from the TCM symptoms were improved and lasts for4weeks until the end of observation (24weeks). Subjects stable vital signs, the medicine treatment group has9cases appear adverse events, adverse event rate by2.5%, and no adverse reactions occurred;Control group has3cases appear adverse events, adverse events incidence2.5%, no adverse reaction occurred. Adverse events and adverse reaction is similar between the two groups has no statistical significance (P>0.05). Conclusion:as the first domestic new agents for the treatment of IGT, TangNaiKang granule can effectively reduce the IGT patients with postprandial blood sugar, and has a tendency to lower glycosylated hemoglobin, significantly and quickly improve clinical symptoms in patients with IGT, decrease the rate of IGT to diabetes outcomes, safe non-toxic, few adverse effects, patient compliance is good.Experimental research part, objective:to observe the main ingredient in sugar resistant kang granules of single taste traditional Chinese medicine (TCM) guava leaf flavonoids on in vitro cultured islet beta cell lines (HIT-T15) intervention mechanism of cellular model of insulin resistance signal pathway. Methods:80male SD rats HIT-T15in vitro culture, divided into normal group, high sugar, insulin stimulation group, guava leaf flavonoids drug-containing serum group, guava leaf flavonoids containing medicine serum group, guava leaf flavonoids containing medicine serum low group, guava leaf flavonoids is high, guava leaf flavonoids in groups, guava leaf flavonoids is low. In turn five experiments:(1) HIT-T15islet beta cells growth curve determination, used to determine the suitable time range of HIT-T15islet beta cells in the experiment;(2) different concentrations of glucose on HIT-T15islet beta cell proliferation, the influence of high sugar model is used to determine the concentration of glucose; Guava leaf flavonoids (3) different concentrations of high sugar HIT-the T15islet beta cell proliferation, used to determine the guava leaf flavonoids best effective stimulus concentration, respectively, using the contain and free serum guava leaf flavonoids, to eliminate the effects of the active factor in serum;(4) insulin secretion analysis, observation of guava leaf flavonoids can improve high sugar HIT-the T15islet beta cells insulin secretion;(5) real time fluorescence quantitative rt-pcr, using relative quantitative method, comparing the HIT-T15islet beta cells themselves group differences in insulin receptor mRNA expression, is used to determine the guava leaf flavonoids to HIT-T15islet beta cells possible targets. Results:guava leaf flavonoids can improve sugar under the stimulus of HIT-T15insulin secretion reduce islet beta cells, improve pancreatic islet beta cells themselves insulin receptor mRNA expression. Conclusion:guava leaf flavonoids can improve the HIT-T15islet beta cells induced by high sugar insulin resistance and its mechanism may be related to improve HIT-T15islet beta cells is related to insulin receptor gene expression. |
PDF Full Text Request