Different Electricity For Chronic Stress Depression Rats Hippocampal Neurotrophic Factors And The Impact Of Glutamic Acid Metabolism Related Proteins

ObjectiveDepression is one of the most common diseases in psychiatric apartment, which has the highest suicide rate. The incidence increased year by year. But the attendance rate is quite low. Suicide and self-inflicted caused by Depression give lots of pain to the families. And it is a large burden for the society. The pathophysiology of depression is not fully understood. The medicines are mainly focused on monoamine neurotransmitter, but it not goes well. Acupuncture is the most popular treatment for the depression for the past few years. A lot of researches proved it can’treat depression in several ways. In our study, the treatment methods are the pulse electro-acupuncture and music electro-acupuncture. The rat is chronic unpredictable mild stress model. In order to find a new target for antidepressant, compare the two kinds of electro acupuncture efficacy.Method and Result1Using open field test, weight test, sugar preference evaluate the model of depression. The open cross, open rear, weight gain, sugar preferences in model group, which are lower than blank control group (P<0.01). This also shows the model of depression is made successfully. The treatment groups are higher than model group (P<0.05). In sugar preferences, the Music electro acupuncture is better than pules acupuncture.2Using nissl body test evaluate the effect of three treatments. Model control group is lower than blank control group, Three treatments all can protect the neure in the hippocampus. Music electro acupuncture is better than pules acupuncture.3Using immunohistochemical methods show the BDNF, and Elisa method show the BDNF, t-PA and PAI-1. Three treatments can stop the reducing of BDNF. Music electro acupuncture is better than Fluoxetine in hippocampus. Three treatments can raise the t-PA/PAI-1, and music elector acupuncture is better than pules acupuncture.4Using immunohistochemical methods show the GFAP, and RT-PCR show the CFAP, EAAT1and EAAT2mRNA. Three treatments all can raise the GFAP, and music electro acupuncture is better than Fluoxetine (P<0.05). Three treatments raise the EAAT1mRNA and EAAT2mRNA (P<0.05).5Using HPLC methods show the Glu, and RT-PCR show the G1uR1, G1uR2, NR1and NR2B mRNA. Fluoxetine raise G1uR2mRNA and reduce NR1, NR2B mRNA (P<0.05). Music electro acupuncture and pules acupuncture can raise GluR1,GluR2mRNA, reduce NR1, NR2B mRNA. Music electro acupuncture is better than pules acupuncture in GluR1, GluR2and NR1mRNA.Conclusion1Behavior results suggest the successful modeling. Fluoxetine and two kinds of electro-acupuncture can improve behavioral performance. Music electro acupuncture is better than the other two treatments in autonomous and exploratory movements. Music electro acupuncture is better than pules acupuncture in weight gain, pleasant sensation and reward mechanism.2The music electro acupuncture for the protective effect of chronic stress depression hippocampal CA1neurons, compared with pules electro-acupuncture, but no significant difference to fluoxetine.3Chronic stress stimulation combined the singly housed cause hippocampal CA1region and serum BDNF levels decreased, fluoxetine, pulse electro-acupuncture and music EA able to reverse the changes. In improving the brain BDNF content, Music electro-acupuncture is superior to fluoxetine and pulse electro-acupuncture. In improving the BDNF levels, music electro-acupuncture is superior to fluoxetine, and pulse electro-acupuncture treatment.4Three treatments can change the GFAP and mRNA in hippocampus. In Improving GFAP and EAAT1, EAAT2mRNA, pulse electro acupuncture and music EA are superior to fluoxetine.5The three intervention can reduce the contents of Glu increased GluR1GluR mRNA reduce NR1and NR2B mRNA. Music electro acupuncture is better than pules acupuncture and fluoxetine in Glu, GluR1, GluR2, NR1mRNA.