Yang Purge Soup Experimental Research For The Treatment Of Slow Both Transmissive Constipation

With social development and to survive the competitive pressure increase,and a change of diet, constipation the incidence of disease is a graduallyincreasing. According to epidemiological information,drawn from severalnational survey data in the United States American incidence of constipation2per cent, and constipation morbidity rate increase over age the increased.Of national epidemiological information displayed at the district rates vary,but are more than2%in China. A survey show that our system for6.07%incidencein the crowd up to15%～20%. System symptoms, not only to people with manytroubles can be concurrent colorectal cancer, in severe cases, the dung of anulcer and urinary retention with other complications. But also Liver braindisease, Early old dementia and other disease incentives. Constipation caneven directly endanger due to induce acute Myocardial infarction orcerebrovascular accident patients lives. Treat constipation drugs more, butmost difficult to take persistent effect, and long-term application is easy tolead to dependence on drugs and side effects that the chance to increase. Soeffective treat constipation. chronic constipation and reduce the adverseeffects caused by nerve system of rate is the medical workers. the importanttasks. Constipation can be divided into primary constipation and liverconstipation. The study of a functional system-slow transport constipation.The traditional chinese medical treatment for constipation gained richexperience, the prevention and control of local transport type that the effectthe system more exact, a distinct advantage. The medical system is divided intothe will and testament, clinical found the local transport system in the twosides to every type of traditional chinese medicine to a secret, particularlyin old age and sex and long-term need a laxative. The course of the disease islonger than the secret, stubborn and clinical symptoms of poor healing. Professor Tian Zhen Guo by years of practice to discover the ancient and modern literature,after a great deal of the verification conclusion for one of the profit oftreatment, and invent Zhuyangtongbian soup to support the bowels in the kidneysand spleen, to treat this disease and have clinical effect.To further explore the treatment mechanism of Zhuyangtongbian soup to treatconstipation. And combine the researching progressiong of internal and external,design the project. Mainly from the following aspects: to observe the study ofthe bowels of the soup with constipation model of the bowels and from themolecular level, the application of group of research on its way to the bindingof this model of the colon the mouse rooms of vessels nervous activity peptide(the vip) and five-ht content, further support of the bowels soup treatconstipation likely impact of the mechanism, for chinese medical treatment forthe slow transfer system provides a basis of clinical experiments, and to providea reference to drug to do well in the preparatory.Experiment1： The slow transfer system, a mouse preparation ofmodelPurpose： Copy of the transport type of rodents slow system modelMaterial and method： The105mice by gender, weight stratification wererandomly divided into normal group, model group, Marenruanjiaonang group、slight-dose Zhuyangtongbian soup group、middle-dose Zhuyangtongbian soupgroup、high-dose Zhuyangtongbian soup group and Xishabili group. A total of7groups,15rats in each group, half male and female. Besides normal group, othergroups are the establishment of model. According to the report, the normal group wasinjected with an equal volume of isotonic saline, the rest of the mice in eachgroup according to the2.5mg/kg dose of once-daily subcutaneous injection ofmorphine hydrochloride, continuous intravenous injection for45days, can causeslow transit constipation mice model.Results：Model of a wizened little mouse appearance, weight, and the small and urinate yellowing, stool stem node obviously, the small particles in the soil,and grain number and weight noticeably, constipation, in the clinical a verysuccessful.Conclusion: On mouse according to2.5mg/kg dose on a subcutaneous injectionof morphine hydrochloride,45days continuous injection method for molding, youcan copy the success of slow transit constipation mice model, simple operation,good safety.The model can be used for further animal experiments using.Experiment2: The influence of Zhuyangtongbian soup on stooltime and stool weight of mousePurpose： To observe the influence of Zhuyangtongbian soup on stool time andstool weight of model mouse,we can judge the efficient effects ofZhuyangtongbian soup on slow transit constipation.Material and method： After injecting mice on a different solutionintragastrically giving.Normal group and model group daily at0.2ml/10g bodyweight with distilled water;Marenruanjiaonang group was intragastic infusedwith Marenruanjiaonang. Xishabili group was intragastic infused with Xishabili.Slight-dose Zhuyangtongbian soup group、middle-dose Zhuyangtongbian soup groupand high-dose Zhuyangtongbian soup group were intragastic infused with differentdose of Zhuyangtongbian soup which contented the medicine of14.63g/kg,29.25g/kg and58.50g/kg. All groups were continuous intragastric administrationfor8days.During the administration, all mice were free of food and drinkingwater, every2days weighing a, and according to the weight change of dosage.Eachgroup of mice in ninth days before drug administration were fasted to restrainwater12h.Ninth days after intragastric administration with30min, each mouseaccording to0.2ml/10g given with good ink gastric perfusion, the end ofoperation at the same time start time.The recovery of normal feeding inlet, andthe filter paper shop on the feeding cages were observed, recorded in mice firstpassed black stool time and each laboratory mice in the24h feces total weight. Results：Compared with the normal group, model group mice first black feces time wassignificantly prolonged (P＜0.05),24h group mouse stool weight weresignificantly reduced (P＜0.05).Compared with the model group, the normal groupand the treated mice first black feces time decreased,24h mice increased stoolweight.Conclusion：1Morphine hydrochloride subcutaneous injection molding method of copyingout mice with constipation model stability.2Zhuyangtongbian soup can shorten the time of mice with constipation firstblack stool, increasing constipation mice24hours of defecation weight, andshowed a dose-effect relationship.Experiment3: The influence of Zhuyangtongbian soup on thelength of ink progradation of the small intestine of mousePurpose： To observe the influence of Zhuyangtongbian soup on the length ofink progradation of the small intestine of mouse,we can judge the efficienteffects of Zhuyangtongbian soup on slow transit constipation.Material and method：In tenth days of mice administered before fasting torestrain water12h, gastric perfusion after administration of30min, the micein each group are in accordance with the0.2ml/10g dose given with good ink.The30min mice were killed (by removal of the cervical approach), calculation ofink in the study of the rate of advance.Methods: after the separation of thesmall and large open abdominal cavity.Measuring the length of intestine for"small total length", from the pylorus to the forefront for ink "ink advancelength", small intestinal propulsive rate is measured the length divided by themeasurement of the length of the latter is multiplied by one hundred percent.Results：The seven groups of mice small bowel intestinal propulsive rate compared to normal mice, model group75.07%, fell to66.53%, the difference wassignificant(P＜0.05); after the treatment of each ink advance rate increasedto different extent, high dose group reached92.52%, significantly higher thanthat in the model group not only, and higher than that in the normal group (P＜0.05).Conclusion：1Morphine hydrochloride subcutaneous injection of1replicate mice withconstipation model stability.2Zhuyangtongbian soup can significantly improve constipation mouse smallintestinal propulsion ratio, strengthening gastrointestinal motility, furtherto cure constipation, and showed a dose-effect relationship.Experiment4:The Effects of Zhuyangtongbian soup on5-HT andVIPPurpose： Zhuyangtongbian soup on study of slow transit constipation modelmice colon tissue pathological changes and5-HT receptor, VIP distribution andexpression of.Material and method：Experiments in mice after the last administrationquickly open abdominal, isolated Escherichia, cutting off the mouse proximalcolon tissue of about15mm, loaded with fixed liquid vials conservation.UsingHE staining and immunohistochemical staining method for dyeing processing, firstobserved under light microscopy in colonic tissue5－HT receptor (5-HT) andvasoactive intestinal peptide (VIP) distribution and content change.Results ofimmunohistochemical determination method: cytoplasm appear yellow or brownishyellow substance5-HT receptor and VIP positive.Results：The amount of5-HT and VIP decreased obviously in colon of mouse modelwith chronic constipation. After treatment by Zhuyangtongbian soup, the amountincreased more than control group. Conclusion： Zhuyangtongbian soup in treatment of slow transit constipationmodel mechanism of action may be regulated through the colon tissueneurotransmitter (5-HT receptors and VIP) content to achieve.